Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Un ringraziamento speciale a Chris Kassotis e Audrey Bailey per la revisione critica di questo manoscritto e il Dr. Scott Korte, Giuseppe Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schroder, Maija Steinberg, e Stacey Winkeler per la loro assistenza per l&#39;ottimizzazione di questo modello nel nostro laboratorio. Il finanziamento è stato fornito dalla formazione di Grant Clinica Biodetectives (NIH T90) (Kep), Università del Missouri Scienze della Vita Undergraduate Research Program Opportunità, MU Research Council, MU Assegni di ricerca Board e NIH R21HD056441 (SCN).

1. Pianificazione per animali vivi chirurgia <ol><li> Assicurano che l&#39;approvazione appropriato è stato ricevuto per lavorare con animali da laboratorio. </li><li> Topi d&#39;ordine e di almeno una settimana di ambientamento al nuovo ambiente. </li><li> Topi di sesso femminile ospitato in assenza di esposizione ai feromoni maschili può fermare il ciclismo, un fenomeno denominato effetto Whitten 15,16. Per mantenere ciclismo topi trasferimento di urina intrisa di biancheria maschile alla gabbia donna o...

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J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Principles+of+Rodent+Surgery+for+the+New+Surgeon.">Principles of Rodent Surgery for the New Surgeon.</a> J. Vis. Exp. (47), e2586-e2586 (2011).</li><li>Moen, M. H., Halvorsen, T. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Histologic+confirmation+of+endometriosis+in+different+peritoneal+lesions.">Histologic confirmation of endometriosis in different peritoneal lesions.</a> Acta. Obstet. Gynecol. Scand. 71, 337-342 (1992).</li><li>Cummings, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Methoxychlor+as+a+model+for+environmental+estrogens.">Methoxychlor as a model for environmental estrogens.</a> Crit. Rev. Toxicol. 27, 367-379 (1997).</li><li>Fowler, R. E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. Reprod. 14, 2944-2950 (1999).</li><li>Hirata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+experimental+model+of+endometriosis+using+mice+that+ubiquitously+express+green+fluorescent+protein.">Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.</a> Hum. Reprod. 20, 2092-2096 (2005).</li><li>Story, L., Kennedy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+studies+in+endometriosis:+a+review.">Animal studies in endometriosis: a review.</a> Ilar. J. 45, 132-138 (2004).</li><li>Cummings, A. M., Hedge, J. M., Birnbaum, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prenatal+exposure+to+TCDD+on+the+promotion+of+endometriotic+lesion+growth+by+TCDD+in+adult+female+rats+and+mice.">Effect of prenatal exposure to TCDD on the promotion of endometriotic lesion growth by TCDD in adult female rats and mice.</a> Toxicol. Sci. 52, 45-49 (1999).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+estrogen,+progesterone,+and+methoxychlor+on+surgically+induced+endometriosis+in+rats.">Effects of estrogen, progesterone, and methoxychlor on surgically induced endometriosis in rats.</a> Fundam. Appl. Toxicol. 27, 287-290 (1995).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. Steril. 88, 1129-1134 (2007).</li><li>Dmitrieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endocannabinoid+involvement+in+endometriosis.">Endocannabinoid involvement in endometriosis.</a> Pain. 151, 703-710 (2010).</li><li>Efstathiou, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonsteroidal+antiinflammatory+drugs+differentially+suppress+endometriosis+in+a+murine+model.">Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model.</a> Fertil. Steril. 83, 171-181 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endostatin+inhibits+the+growth+of+endometriotic+lesions+but+does+not+affect+fertility.">Endostatin inhibits the growth of endometriotic lesions but does not affect fertility.</a> Fertil. Steril. 84, 1144-1155 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+synthetic+endostatin+peptides+inhibit+endothelial+migration+in+vitro+and+endometriosis+in+a+mouse+model.">Short synthetic endostatin peptides inhibit endothelial migration in vitro and endometriosis in a mouse model.</a> Fertil. Steril. 85, 71-77 (2006).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+rats+as+a+research+model+for+the+study+of+endometriosis.">Using rats as a research model for the study of endometriosis.</a> Ann. N.Y. Acad. Sci. 955, 318-327 (2002).</li><li>Stilley, J. A., Woods-Marshall, R., Sutovsky, M., Sutovsky, P., Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+Fecundity+in+Female+Rats+with+Surgically+Induced+Endometriosis+and+in+Their+Daughters:+A+Potential+Role+for+Tissue+Inhibitors+of+Metalloproteinase+1.">Reduced Fecundity in Female Rats with Surgically Induced Endometriosis and in Their Daughters: A Potential Role for Tissue Inhibitors of Metalloproteinase 1.</a> Biol. Reprod. 80, (2009).</li></ol>

Ci sono diversi parametri critici che dovrebbero essere notato durante l&#39;esecuzione l&#39;induzione chirurgica di endometriosi nei topi. In primo luogo, l&#39;endometriosi è una malattia estrogeno-dipendenti e come tale questo tipo di chirurgia deve essere eseguita nell&#39;animale intatto o in alternativa negli animali ovariectomizzati integrato con estrogeni 20. In secondo luogo, la sutura biopsie endometriali alla cascata arteriosa deve essere eseguita con estrema cura. Abbiamo scoperto che usando sol...

<table border="1"><tbody><tr><td> Nome del reagente / apparecchiatura </td><td> Azienda </td><td> Numero di catalogo </td></tr><tr><td> Matita di cera </td><td> Pescatore </td><td> NC9954135 </td></tr><tr><td> Vetrini </td><td> Pescatore </td><td> 12-550-433 </td></tr><tr><td> Contagocce </td><td> Pescatore </td><td> S79383 </td></tr><tr><td> Microscopio ottico standard per la valutazione strisci citologia vaginale </td><td></td><td></td></tr><tr><td> Buprenorfina HCL c3 (CARJET) 10X1ml </td><td> Butler animali Alimentazione Salute </td><td> 022891 </td></tr><tr><td> Fosfato sterile salina tamponata (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10.000 U / ml penicillina, 10.000 mg / ml di streptomicina in 0,85% NaCl </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isoflurano </td><td> AbbottAnimal Health </td><td> 05260-05 </td></tr><tr><td> Isoflurano non rirespirazione anestetico sistema </td><td></td><td></td></tr><tr><td> Ricircolo di acqua calda di riscaldamento pad </td><td></td><td></td></tr><tr><td> 30 ml siringa guaina </td><td> Pescatore </td><td> 14-823-16G </td></tr><tr><td> Polvere di guanti sterili libero </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Unguento oftalmico </td><td> Farmaceutica principali </td><td> 10033691 </td></tr><tr><td> Piccolo tagliaunghie elettrici </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Clorexidina macchia </td><td> Pescatore </td><td> NC9863042 </td></tr><tr><td> 70% di etanolo </td><td></td><td></td></tr><tr><td> Polylined campo sterile </td><td> Busse Ospedale Disposables </td><td> 696 </td></tr><tr><td> Taglia 3 bisturi </td><td> Pescatore </td><td> 22-079-657 </td></tr><tr><td> Numero 10 lame bisturi </td><td> Pescatore </td><td> 22-079-681 </td></tr><tr><td> Piccole forbici chirurgiche </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Piccoli semi-curve seghettate forcipe </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 di seta nera intrecciata sutura </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Sterilizzato vetro pirex Petri </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 millimetri biopsia </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Garza sterile </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 nero ethilon sutura monofilo di nylon </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Driver ago (opzionale) </td><td> Mondo Strumenti di precisione </td><td> 500023 </td></tr><tr><td> 5-0 undyed intrecciato rivestito vicryl sutura </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9 millimetri Autoclip ferita clip </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip applicatore e di rimozione </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> 23G ago </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> 1cc siringa </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5X vetro stare ingrandimento (opzionale) </td><td> Pescatore </td><td> 14-648-23 </td></tr><tr><td> 10% di formalina tamponata </td><td> Pescatore </td><td> SF100-4 </td></tr><tr><td> Pinze </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Elaborazione / embedding cassette </td><td> Pescatore </td><td> 15-197-700A </td></tr><tr><td> Biopsia cuscinetti in schiuma </td><td> Pescatore </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA Kit di isolamento </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Azoto liquido </td><td></td><td></td></tr><tr><td> Snap microcentrifuga coppola tubo orizzontale </td><td> Pescatore </td><td> 02-681-240 </td></tr><tr><td> Ketamina (opzionale) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (medetomidina cloridrato) (opzionale) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezolo) (opzionale) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

L&#39;endometriosi è una malattia cronica, dolorosa malattia la cui eziologia rimane sconosciuta. Inoltre, il trattamento di endometriosi può richiedere la rimozione laparoscopica delle lesioni, e / o croniche gestione farmaceutica del dolore e dei sintomi infertilità. Il costo associato con endometriosi è stato stimato a 22 miliardi di dollari all&#39;anno negli Stati Uniti 1. Per ulteriormente la nostra comprensione dei meccanismi alla base di questa malattia enigmatica, i modelli animali sono stati impiegati. Primati sviluppano spontaneamente endometriosi e quindi modelli di primati più simili alla malattia nelle donne. Modelli di roditori, tuttavia, sono più convenienti e facilmente reperibili 2. Il modello che abbiamo descritto qui comporta un trasferimento autologo di tessuto uterino al mesentere intestinale (Figura 1) ed è stato sviluppato nel ratto 3 e poi trasferito al mouse 4. L&#39;obiettivo del modello roditore autologo di endometriosi chirurgicamente indotta è quella di imitarela malattia nelle donne. Noi e altri hanno già dimostrato che l&#39;alterato pattern di espressione genica osservata nelle lesioni endometriosiche da topi o ratti rispecchia quella osservata nelle donne con la malattia 5,6. Uno dei vantaggi del realizzare l&#39;intervento nel topo è che l&#39;abbondanza di ceppi di topi transgenici disponibile può aiutare i ricercatori a determinare il ruolo di specifici componenti importanti nella creazione e la crescita di endometriosi. Un modello alternativo in cui vengono introdotti asportati frammenti umani endometriale al peritoneo di topi immunocompromessi è anche ampiamente utilizzato, ma è limitata dalla mancanza di un normale sistema immunitario che si ritiene essere importante per l&#39;endometriosi 2,7. È importante sottolineare che il modello murino di endometriosi indotta chirurgicamente è un modello versatile che è stato utilizzato per studiare come il sistema immunitario 8, 9,10 ormoni e fattori ambientali influenzano 11,12 endometriosi così come gli effetti dell&#39;endometriosi sulla Fertility 13 e il dolore 14.

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Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Una descrizione della induzione chirurgica di endometriosi in topi e ratti con auto-trapianto di tessuto uterino alla cascata delle arterie del mesentere intestinale.

Modello murino di endometriosi chirurgicamente indotto da Auto-trapianto di tessuto uterino

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Modello murino di MCAO intraluminale: valutazione infarto cerebrale da colorazione viola cresile

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

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Medicina

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

L&#39;impianto di Vena cava inferiore Interposizione Graft in modello murino

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Il trapianto di valvola polmonare Utilizzando un modello murino di trapianto cardiaco eterotopico

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Rosa Bengala Photothrombosis da confocale Optical Imaging<em&gt; In Vivo</em&gt;: Un modello di singolo vaso Stroke

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Valutazione del Cardiac morfologiche e funzionali Variazioni modello murino di trasversale aortica costrizione da parte ecocardiografica Imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Creazione di un modello del roditore del Aneurysm aortico addominale bloccando Adventitial Vasa Vasorum perfusione

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

Misurazioni di contrattilità della muscolatura liscia uterina umana per lo sviluppo di farmaci di aiuto

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

Un modello murino di chirurgia ortopedica per studiare la disfunzione cognitiva postoperatoria e rigenerazione tissutale

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Miglioramento di un modello di infarto miocardico porcina cassa chiusa tramite la normalizzazione del tessuto e le procedure di campionamento di sangue

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Misurazione di contrattilità isometrica dell'arteria mesenterica Mouse con filo Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...