Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

최적화에서 지원 크리스 Kassotis이 원고의 중요한 검토 오드리 베일리와 박사 스콧 Korte, 요셉 Beeman, 앨리슨 Curfman, 폴 Kimball, 브리짓 Neibreggue, 야곱 Redel, 에이미 Schroder, Maija 스테인 버그, 그리고 스테이시 Winkeler 특별 감사 우리 연구실이 모델. 자금은 임상 Biodetectives 교육 그랜트 (NIH T90) (KEP), 미주리 생명 과학 대학 학부 연구 기회 프로그램, MU 연구위원회, 연구위원회 MU 부여와 NIH R21HD056441 (SCN)에 의해 제공되었다.

1. 라이브 - 동물 수술 계획 <ol><li> 적절한 승인이 실험실 동물에서 작동하도록 수신되었는지 확인하십시오. </li><li> 주문 마우스 및 새로운 환경에 acclimation 중 하나 이상 주 수 있습니다. </li><li> 남성 페로몬에 노출의 부재에 보관되어 여성 쥐가 자전거를 막을 수 현상이 Whitten 효과 15,16이라고도합니다. 쥐 자전거 전송 소변 - 절어 남성 침구 여성 케이지마다 오일 유지합니다. 또한, 오픈...

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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+rats+as+a+research+model+for+the+study+of+endometriosis.">Using rats as a research model for the study of endometriosis.</a> Ann. N.Y. Acad. Sci. 955, 318-327 (2002).</li><li>Stilley, J. A., Woods-Marshall, R., Sutovsky, M., Sutovsky, P., Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reduced+Fecundity+in+Female+Rats+with+Surgically+Induced+Endometriosis+and+in+Their+Daughters:+A+Potential+Role+for+Tissue+Inhibitors+of+Metalloproteinase+1.">Reduced Fecundity in Female Rats with Surgically Induced Endometriosis and in Their Daughters: A Potential Role for Tissue Inhibitors of Metalloproteinase 1.</a> Biol. Reprod. 80, (2009).</li></ol>

생쥐에서 endometriosis의 수술 유도를 수행하는 동안 주목한다 몇 가지 중요한 매개 변수가 있습니다. 첫째, endometriosis는 에스 트로겐에 의존 질병 등이 수술로 손상 동물이나 또는 estrogens에 20 보충 ovariectomized 동물 수행해야합니다. 둘째, 동맥 폭포로 자궁 biopsies를 suturing 것은 극도의주의 수행해야합니다. 우리는 하나 두 비교적 느슨한 매듭을 사용하면 변의와 후속 조직 괴사 및 동물 죽음...

<table border="1"><tbody><tr><td> 시약 / 장비의 명칭 </td><td> 회사 </td><td> 카탈로그 번호 </td></tr><tr><td> 왁스 연필 </td><td> 어부 </td><td> NC9954135 </td></tr><tr><td> 유리 슬라이드 </td><td> 어부 </td><td> 12-550-433 </td></tr><tr><td> 스포이트 </td><td> 어부 </td><td> S79383 </td></tr><tr><td> 질 세포학 얼룩을 평가하기위한 표준 가벼운 현미경 </td><td></td><td></td></tr><tr><td> Buprenorphine HCL C3 (CARJET) 10X1ml </td><td> 버틀러 동물 건강 공급 </td><td> 022,891 </td></tr><tr><td> 멸균 인산은 버퍼링 식염수 (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10,000 U / ML 페니실린, 0.85 % NaCl 10,000 μg / ML 스트렙토 마이신 </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isoflurane </td><td> 애보트동물 건강 </td><td> 05260-05 </td></tr><tr><td> Isoflurane 아닌 rebreathing 마취 시스템 </td><td></td><td></td></tr><tr><td> 순환 온수 난방 패드 </td><td></td><td></td></tr><tr><td> 30 ML 주사기의 드레스 </td><td> 어부 </td><td> 14-823 - 16G </td></tr><tr><td> 파우더 무료 무균 장갑 </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> 안과 연고 </td><td> 주요 제약 </td><td> 10033691 </td></tr><tr><td> 소형 전기 면도기 </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Chlorhexidine 스크럽 </td><td> 어부 </td><td> NC9863042 </td></tr><tr><td> 70 % 에탄올 </td><td></td><td></td></tr><tr><td> Polylined 무균 분야 </td><td> Busse 병원 Disposables </td><td> 696 </td></tr><tr><td> 크기 3 메스 </td><td> 어부 </td><td> 22-079-657 </td></tr><tr><td> 10 번 메스 블레이드 </td><td> 어부 </td><td> 22-079-681 </td></tr><tr><td> 중소 외과 가위 </td><td> Roboz </td><td> RS - 5850 </td></tr><tr><td> 작은 톱니 모양의 세미 곡선 포셉 </td><td> Roboz </td><td> RS - 5135 </td></tr><tr><td> 5-0 검은 꼰 실크 봉합사 </td><td> Ethicon </td><td> K870H </td></tr><tr><td> 멸균 pyrex 유리 배양 접시 </td><td> 코닝 </td><td> 70160-101 </td></tr><tr><td> 2mm 생검 펀치 </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> 멸균 거즈 </td><td> 켄달 </td><td> 1806 </td></tr><tr><td> 6-0 검은 monofilament의 ethilon 나일론 봉합 </td><td> Ethicon </td><td> 697G </td></tr><tr><td> 니들 드라이버 (옵션) </td><td> 세계 정밀 계측기 </td><td> 500,023 </td></tr><tr><td> 5-0 undyed 꼰 코팅 Vicryl 봉합사 </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip이 클립을 상처 </td><td> Becton 디킨슨 </td><td> 427,631 </td></tr><tr><td> Autoclip applier 및 제거 </td><td> Becton 디킨슨 </td><td> 427,630 </td></tr><tr><td> 23G 바늘 </td><td> Becton 디킨슨 </td><td> 305,193 </td></tr><tr><td> 1cc 주사기 </td><td> Becton 딕슨 </td><td> 301,025 </td></tr><tr><td> 배 돋보기 스탠드 (옵션) </td><td> 어부 </td><td> 14-648-23 </td></tr><tr><td> 10 % 포르말린 버퍼 </td><td> 어부 </td><td> SF100 - 4 </td></tr><tr><td> 캘리퍼스 </td><td> Roboz </td><td> RS - 6466 </td></tr><tr><td> 카세트 처리 / 삽입 </td><td> 어부 </td><td> 15-197 - 700A </td></tr><tr><td> 생검 거품 패드 </td><td> 어부 </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA 분리 키트 </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> 액체 질소 </td><td></td><td></td></tr><tr><td> 스냅 캡 microcentrifuge 평면 상단 튜브 </td><td> 어부 </td><td> 02-681-240 </td></tr><tr><td> 케타민을 (옵션) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (medetomidine 하이드로 클로라이드) (옵션) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezole) (옵션) </td><td> 시그마 </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

Endometriosis 누구의 병인 알려지지 않은 남아 만성, 고통스러운 질병입니다. 또한, endometriosis의 치료 복강경 병변의 제거, 및 / 또는 통증과 불임 현상 만성 제약 관리를 요구할 수 있습니다. endometriosis와 관련된 비용은 미국 1 연간 22,000,000,000 달러로 추산되었습니다. 이 수수께끼의 질병을 기본 메커니즘에 대한 우리의 이해를 더하기 위해 동물 모델은 고용되었습니다. 영장류는 자발적으로 endometriosis를 개발하기 때문에 영장류 모델은 가장 밀접하게 여성 질환과 유사. 설치류 모델은, 그러나, 효과적인 비용과 2 쉽게 사용할 수 있습니다. 우리가 여기서 설명하는 모델은 장의 장간막 (그림 1) 자궁 조직의 autologous 전송을 포함하고 먼저 쥐 3 개발하고 나중에 마우스 4 전송되었습니다. 수술 유도 endometriosis의 autologous 설치류 모델의 목적은 모방하는 것입니다여성의 질병. 우리와 다른 이전에 변경 유전자 발현 패턴이 마우스 또는 질병 5,6있는 여성에서 관찰 쥐의 미러에서 endometriotic 병변에서 관찰하는 것으로 나타났습니다. 마우스에서 수술을 집도할 중 하나 장점은 가능한 유전자 변형 마우스 종자의 풍부한은 설치 및 endometriosis의 성장에 중요한 특정 구성 요소의 역할을 결정하는 연구자 도움 수있다는 것입니다. excised 인간 자궁 내막 조각이 immunocompromised 생쥐의 복막을 소개하는 대안 모델도 널리 사용되고 있지만 endometriosis 2,7에서 중요한 것으로 생각되는 정상적인 면역 체계의 부족에 의해 제한됩니다. 중요한 것은 수술 유도 endometriosis의 마우스 모델은 면역 체계 8, 9,10 호르몬과 환경 요인 11,12가 fert에 endometriosis뿐만 아니라 endometriosis의 효과에 미치는 영향 연구하는 데 사용되었습니다 다재 다능한 모델입니다ility 13 고통 14.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

장 장간막의 동맥 계단식으로 자궁 조직의 자동 이식에 의한 마우스 및 쥐에 endometriosis의 수술 유도의 설명입니다.

자궁의 자동 이식하여 수술 유발 Endometriosis의 마우스 모델

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

Research

JoVE Journal

Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Intraluminal MCAO의 마우스 모델 : Cresyl 보라 착색에 의한 뇌 경색 평가

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

연구

의학

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

마우스 모델에서 하대 정맥 끼어 이식의 주입

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

이소성 심장 이식 마우스 모델을 사용하여 폐동맥 판막 이식

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

공 초점 광학 이미징에 의해 로즈 벵갈 Photothrombosis<em&gt; 생체</em&gt; : 단일 선박 스트로크의 모델

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

심 초음파 이미징에 의해 가로 대동맥 수축의 마우스 모델에서 심장 형태 학적 평가 및 기능 변경

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Adventitial 바사 Vasorum 관류를 차단 하 여 복 부 대동맥 동맥 류의 설치류 모델의 생성

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

보조 약물 개발에 인간 자 궁 평활 근의 수축 측정

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

정형 외과 수술 후 인지 기능 장애 및 조직 재생 연구의 마우스 모델

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

조직 및 혈액 샘플링 절차의 표준화에 의해 닫힌된 가슴 돼지 심근 경색 모델의 개선

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Myography 와이어를 사용 하 여 마우스 Mesenteric 동맥의 isometric 수축 측정

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...