Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Spesiell takk til Chris Kassotis og Audrey Bailey for kritisk gjennomgang av dette manuskriptet og til Dr. Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schroder, Maija Steinberg, og Stacey Winkeler for deres assistanse i optimering av denne modellen i vårt laboratorium. Finansieringen ble gitt av Clinical Biodetectives Training Grant (NIH T90) (KEP), University of Missouri biovitenskap Undergraduate Forskning Opportunities Program, MU Forskningsrådet, MU Forskning Board tilskudd og NIH R21HD056441 (SCN).

1. Planlegging for live-dyr kirurgi <ol><li> Sørge for at nødvendige godkjenning er mottatt til å arbeide med forsøksdyr. </li><li> Order mus og la minst en ukes akklimatisering til sitt nye miljø. </li><li> Hunnmus ligger i mangel av eksponering for mannlige feromoner kan stoppe sykling, referert et fenomen til som Whitten effekten 15,16. For å holde mus sykling overføre urin-gjennomvåt mannlige sengetøy til den kvinnelige buret hver fem dager. Alternativt, hvis åpen topp bur brukes, plasser den ...

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	<li>Simoens, S., Hummelshoj, L., D'Hooghe, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis:+cost+estimates+and+methodological+perspective.">Endometriosis: cost estimates and methodological perspective.</a> Hum. Reprod. Update. 13, 395-404 (2007).</li><li>Grummer, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+in+endometriosis+research.">Animal models in endometriosis research.</a> Hum. Reprod. Update. 12, 641-649 (2006).</li><li>Vernon, M. W., Wilson, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Studies+on+the+surgical+induction+of+endometriosis+in+the+rat.">Studies on the surgical induction of endometriosis in the rat.</a> Fertil. 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Steril. 93, 1615-1627 (2010).</li><li>Flores, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecular+profiling+of+experimental+endometriosis+identified+gene+expression+patterns+in+common+with+human+disease.">Molecular profiling of experimental endometriosis identified gene expression patterns in common with human disease.</a> Fertil. Steril. 87, 1180-1199 (2007).</li><li>Giudice, L. C., Kao, L. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriosis.">Endometriosis.</a> Lancet. 364, 1789-1799 (2004).</li><li>Lin, Y. J., Lai, L. e. i., Y, H., Wing, L. 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E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. Reprod. 14, 2944-2950 (1999).</li><li>Hirata, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+experimental+model+of+endometriosis+using+mice+that+ubiquitously+express+green+fluorescent+protein.">Development of an experimental model of endometriosis using mice that ubiquitously express green fluorescent protein.</a> Hum. Reprod. 20, 2092-2096 (2005).</li><li>Story, L., Kennedy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+studies+in+endometriosis:+a+review.">Animal studies in endometriosis: a review.</a> Ilar. J. 45, 132-138 (2004).</li><li>Cummings, A. M., Hedge, J. M., Birnbaum, L. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+prenatal+exposure+to+TCDD+on+the+promotion+of+endometriotic+lesion+growth+by+TCDD+in+adult+female+rats+and+mice.">Effect of prenatal exposure to TCDD on the promotion of endometriotic lesion growth by TCDD in adult female rats and mice.</a> Toxicol. Sci. 52, 45-49 (1999).</li><li>Cummings, A. M., Metcalf, J. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+estrogen,+progesterone,+and+methoxychlor+on+surgically+induced+endometriosis+in+rats.">Effects of estrogen, progesterone, and methoxychlor on surgically induced endometriosis in rats.</a> Fundam. Appl. Toxicol. 27, 287-290 (1995).</li><li>Sharpe-Timms, K. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. Steril. 88, 1129-1134 (2007).</li><li>Dmitrieva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endocannabinoid+involvement+in+endometriosis.">Endocannabinoid involvement in endometriosis.</a> Pain. 151, 703-710 (2010).</li><li>Efstathiou, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nonsteroidal+antiinflammatory+drugs+differentially+suppress+endometriosis+in+a+murine+model.">Nonsteroidal antiinflammatory drugs differentially suppress endometriosis in a murine model.</a> Fertil. Steril. 83, 171-181 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endostatin+inhibits+the+growth+of+endometriotic+lesions+but+does+not+affect+fertility.">Endostatin inhibits the growth of endometriotic lesions but does not affect fertility.</a> Fertil. Steril. 84, 1144-1155 (2005).</li><li>Becker, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+synthetic+endostatin+peptides+inhibit+endothelial+migration+in+vitro+and+endometriosis+in+a+mouse+model.">Short synthetic endostatin peptides inhibit endothelial migration in vitro and endometriosis in a mouse model.</a> Fertil. Steril. 85, 71-77 (2006).</li><li>Sharpe-Timms, K. 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Det er flere kritiske parametre som bør bemerkes mens du utfører kirurgisk induksjon av endometriose hos mus. Først er endometriose en østrogenavhengige sykdom og som sådan denne operasjonen skal utføres i intakt dyr eller alternativt i ovariectomized dyr supplert med østrogener 20. For det andre må suturering endometrial biopsier til arterielle cascade utføres med ekstrem varsomhet. Vi har funnet ut at å bruke bare to relativt løse knuter med ett kast hver holder biopsi på plass mens hindre ligat...

<table border="1"><tbody><tr><td> Navn på reagens / utstyr </td><td> Firma </td><td> Katalognummer </td></tr><tr><td> Wax blyant </td><td> Fisher </td><td> NC9954135 </td></tr><tr><td> Glass-slide </td><td> Fisher </td><td> 12-550-433 </td></tr><tr><td> Pipetteikonet </td><td> Fisher </td><td> S79383 </td></tr><tr><td> Standard lysmikroskop for å vurdere vaginal cytologi utstryk </td><td></td><td></td></tr><tr><td> Buprenorfin HCL c3 (CARJET) 10X1ml </td><td> Butler Animal Health forsyning </td><td> 022891 </td></tr><tr><td> Sterile fosfatbufret saltløsning (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10 000 U / ml Penicillin, 10.000 mikrogram / ml Streptomycin i 0,85% NaCl </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isofluran </td><td> AbbottAnimal Health </td><td> 05260-05 </td></tr><tr><td> Isofluran non-gjeninnånding bedøvelse system </td><td></td><td></td></tr><tr><td> Resirkulering varmtvann pad </td><td></td><td></td></tr><tr><td> 30 ml sprøyte slire </td><td> Fisher </td><td> 14-823-16G </td></tr><tr><td> Powder gratis sterile hansker </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Ophthalmic salve </td><td> Major Farmasi </td><td> 10033691 </td></tr><tr><td> Små elektriske klippere </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Klorheksidin skrubbe </td><td> Fisher </td><td> NC9863042 </td></tr><tr><td> 70% Etanol </td><td></td><td></td></tr><tr><td> Polylined sterile feltet </td><td> Busse Hospital Disposables </td><td> 696 </td></tr><tr><td> Størrelse 3 skalpell </td><td> Fisher </td><td> 22-079-657 </td></tr><tr><td> Nummer 10 skalpell blader </td><td> Fisher </td><td> 22-079-681 </td></tr><tr><td> Små kirurgisk saks </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Små taggete semi-buet tang </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 sort flettet silke sutur </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Sterilisert Pyrex glass petriskåler </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm biopsi dor </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Sterilt gasbind </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 svart monofilament ethilon nylon sutur </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Needle drivere (valgfritt) </td><td> Verden presisjonsinstrumenter </td><td> 500023 </td></tr><tr><td> 5-0 ufarvet flettet belagt vicryl suture </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm Autoclip sår klipp </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip applier &amp; remover </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> 23G nål </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> 1cc sprøyte </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5X forstørrelsesglasset stativ (ekstrautstyr) </td><td> Fisher </td><td> 14-648-23 </td></tr><tr><td> 10% bufret formalin </td><td> Fisher </td><td> SF100-4 </td></tr><tr><td> Calipers </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Processing / embedding kassetter </td><td> Fisher </td><td> 15-197-700A </td></tr><tr><td> Biopsi skum pads </td><td> Fisher </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA isolering kit </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Flytende nitrogen </td><td></td><td></td></tr><tr><td> Snap cap mikrosentrifuge flat topp tube </td><td> Fisher </td><td> 02-681-240 </td></tr><tr><td> Ketamin (valgfritt) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (medetomidin hydroklorid) (valgfritt) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezol) (valgfritt) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

Endometriose er en kronisk, smertefull sykdom hvis etiologi er ukjent. Videre kan behandling av endometriose krever laparoskopisk fjerning av lesjoner, og / eller kronisk farmasøytisk behandling av smerter og infertilitet symptomer. Kostnaden forbundet med endometriose har vært anslått til 22 milliarder dollar per år i USA 1. For ytterligere vår forståelse av mekanismene bak denne gåtefulle sykdommen har dyremodeller vært ansatt. Primater spontant utvikler endometriose og derfor primate modeller mest ligner sykdommen hos kvinner. Rodent modeller, derimot, er mer kostnadseffektiv og lett tilgjengelig 2. Den modellen som vi beskriver her innebærer en autolog overføring av livmor vev til intestinal mesenteriet (figur 1) og ble først utviklet i rotte 3 og senere overført til musen fire. Målet med autolog gnager modell av kirurgisk indusert endometriose er å etterlignesykdommen hos kvinner. Vi og andre har tidligere vist at endret genuttrykk mønsteret observert i endometriotic lesjoner fra mus eller rotter speil som ble observert hos kvinner med sykdommen 5,6. En fordel med å utføre operasjonen i musen er at overflod av transgene mus stammer tilgjengelig kan hjelpe forskerne med å bestemme hvilken rolle spesifikke komponenter viktig i etablering og vekst av endometriose. En alternativ modell der excised menneskelige endometrial fragmenter blir introdusert til peritoneum av immunsupprimerte mus er også mye brukt, men er begrenset av mangelen på et normalt immunforsvar som antas å være viktig i endometriose 2,7. Viktigere er det mus modell av kirurgisk indusert Endometriose en allsidig modell som har blitt brukt til å studere hvordan immunsystemet 8, hormoner 9,10 og miljøfaktorer 11,12 påvirke endometriose samt effekten av endometriose på Fertility 13 og smerte 14.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

En beskrivelse av kirurgisk induksjon av endometriose hos mus og rotter ved auto-transplantasjon av livmor vev til arteriell kaskade av intestinal mesenteriet.

Mus Modell av kirurgisk indusert Endometriose av Auto-transplantasjon av livmor Tissue

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Transplantasjon av Pulmonary Valve Bruke en mus modell av Heterotop hjertetransplantasjon

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Rose Bengal Photothrombosis av Confocal Optical Imaging<em&gt; I Vivo</em&gt;: En modell av enkelt Vessel Stroke

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Vurdering av Cardiac morfologiske og funksjonelle endringer i musemodell for Tverr aortic Innsnevring av ekkokardiografi Imaging

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Etableringen av en gnager modell av abdominalt aortaaneurisme blokkerer Adventitial Vasa Vasorum perfusjon

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

Contractility målinger av menneskelig livmor glatt muskel Aid narkotika utvikling

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

En musemodell av Ortopedisk kirurgi postoperativ Cognitive Dysfunction og vev gjenfødelse

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Forbedring av en lukket kiste svin hjerteinfarkt modell av standardisering av vev og blod utvalgstrekking

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Isometrisk Contractility måling av musen hvem arterien bruker Wire Myography

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...