Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Agradecimentos especiais a Chris Bailey Kassotis e Audrey para a revisão crítica do manuscrito e ao Dr. Scott Korte, Beeman Joseph, Curfman Alison, Kimball Paulo, Neibreggue Bridget, Redel Jacob, Schroder Amy, Steinberg Maija e Winkeler Stacey, o auxílio na otimização deste modelo em nosso laboratório. O financiamento foi fornecido pela Clínica Biodetectives Training Grant (NIH T90) (KEP), University of Missouri Life Sciences Research Graduação Programa de Oportunidades, Pesquisa MU Conselho, Research Board MU subvenções e R21HD056441 NIH (SCN).

1. Planejamento para a cirurgia de animais vivos <ol><li> Certifique-se que a aprovação apropriada foi recebido para trabalhar com animais de laboratório. </li><li> Camundongos ordem e permitir que pelo menos uma semana de aclimatação ao novo ambiente. </li><li> Camundongos fêmeas alojadas na ausência de exposição a feromônios do sexo masculino pode parar de pedalar, um fenômeno conhecido como o efeito Whitten 15,16. Para manter os ratos ciclismo transferência da cama encharcada de urina masculi...

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E., Edwards, R. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+superovulation+and+pregnancy+in+mature+mice+by+gonadotrophins.">Induction of superovulation and pregnancy in mature mice by gonadotrophins.</a> J. Endocrinol. 15, 374-384 (1957).</li><li>Wilson, E. D., Zarrow, M. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+superovulation+in+the+immature+mouse+and+rat.">Comparison of superovulation in the immature mouse and rat.</a> J. Reprod. Fertil. 3, 148-158 (1962).</li><li>Lee, B., Du, H., Taylor, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+murine+endometriosis+induces+DNA+methylation+and+altered+gene+expression+in+eutopic+endometrium.">Experimental murine endometriosis induces DNA methylation and altered gene expression in eutopic endometrium.</a> Biol. Reprod. 80, 79-85 (2009).</li><li>Somigliana, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometrial+ability+to+implant+in+ectopic+sites+can+be+prevented+by+interleukin-12+in+a+murine+model+of+endometriosis.">Endometrial ability to implant in ectopic sites can be prevented by interleukin-12 in a murine model of endometriosis.</a> Hum. 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L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Endometriotic+lesions+synthesize+and+secrete+a+haptoglobin-like+protein.">Endometriotic lesions synthesize and secrete a haptoglobin-like protein.</a> Biol. Reprod. 58, 988-994 (1998).</li><li>Yavuz, E., Oktem, M., Esinler, I., Toru, S. A., Zeyneloglu, H. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genistein+causes+regression+of+endometriotic+implants+in+the+rat+model.">Genistein causes regression of endometriotic implants in the rat model.</a> Fertil. 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Existem vários parâmetros críticos que devem ser observadas durante a realização da indução cirúrgica de endometriose em camundongos. Primeiro, a endometriose é uma doença estrogênio dependente e, como tal, esta cirurgia deve ser realizada em animais intactos ou, alternativamente, em animais ovariectomizados suplementado com estrógenos 20. Segundo, suturar as biópsias endometriais para a cascata arterial deve ser realizada com extremo cuidado. Nós descobrimos que o uso de apenas dois nós relati...

<table border="1"><tbody><tr><td> Nome do reagente / equipamentos </td><td> Companhia </td><td> Número de catálogo </td></tr><tr><td> Lápis de cera </td><td> Pescador </td><td> NC9954135 </td></tr><tr><td> Lâmina de vidro </td><td> Pescador </td><td> 12-550-433 </td></tr><tr><td> Conta-gotas </td><td> Pescador </td><td> S79383 </td></tr><tr><td> Microscópio de luz padrão para avaliar manchas de citologia vaginal </td><td></td><td></td></tr><tr><td> Buprenorfina HCL c3 (CARJET) 10X1ml </td><td> Butler Abastecimento da Saúde Animal </td><td> 022891 </td></tr><tr><td> Fosfato estéril salina tamponada (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10.000 U / ml de penicilina, estreptomicina 10.000 mg / ml em NaCl 0,85% </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> Isoflurano </td><td> AbbottSaúde Animal </td><td> 05260-05 </td></tr><tr><td> Isoflurano não-reinalação sistema anestésico </td><td></td><td></td></tr><tr><td> Recirculação almofada de aquecimento de água quente </td><td></td><td></td></tr><tr><td> 30 bainha seringa ml </td><td> Pescador </td><td> 14-823-16G </td></tr><tr><td> Pó livre luvas estéreis </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Pomada oftálmica </td><td> Pharmaceuticals principais </td><td> 10033691 </td></tr><tr><td> Pequenas tesouras elétricas </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Matagal clorexidina </td><td> Pescador </td><td> NC9863042 </td></tr><tr><td> Etanol 70% </td><td></td><td></td></tr><tr><td> Campo estéril Polylined </td><td> Hospital Descartáveis ​​Busse </td><td> 696 </td></tr><tr><td> Tamanho 3 bisturi </td><td> Pescador </td><td> 22-079-657 </td></tr><tr><td> Número de 10 lâminas de bisturi </td><td> Pescador </td><td> 22-079-681 </td></tr><tr><td> Pequenas tesouras cirúrgicas </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Pequenas dentadas semi-curvada forceps </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 de sutura de seda preta trançada </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Esterilizado pirex de vidro placas de Petri </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 milímetros biópsia com punch </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Gaze estéril </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 preto sutura de monofilamento nylon ethilon </td><td> Ethicon </td><td> 697G </td></tr><tr><td> Drivers de agulha (opcional) </td><td> Precision Instruments mundo </td><td> 500023 </td></tr><tr><td> 5-0 undyed trançado revestido vicryl sutura </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9 milímetros Autoclip ferida clips </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> Autoclip aplicador e removedor </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> Agulha 23G </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> Seringa 1cc </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> Estande de vidro 5X de aumento (opcional) </td><td> Pescador </td><td> 14-648-23 </td></tr><tr><td> 10% de formalina tamponada </td><td> Pescador </td><td> SF100-4 </td></tr><tr><td> Calibrador </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> Processamento / incorporação cassetes </td><td> Pescador </td><td> 15-197-700A </td></tr><tr><td> Almofadas de espuma de biópsia </td><td> Pescador </td><td> 22-038-222 </td></tr><tr><td> Kit de isolamento RNAqueous RNA </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Nitrogênio líquido </td><td></td><td></td></tr><tr><td> Microcentrífuga tirar tampa do tubo topo plano </td><td> Pescador </td><td> 02-681-240 </td></tr><tr><td> Ketamina (opcional) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (cloridrato de medetomidina) (opcional) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezol) (opcional) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

A endometriose é uma doença crônica e dolorosa cuja etiologia permanece desconhecida. Além disso, o tratamento da endometriose pode exigir a remoção laparoscópica de lesões e / ou gestão farmacêutica crônico de dor e os sintomas de infertilidade. O custo associado com endometriose tem sido estimado em 22 bilhões de dólares por ano nos Estados Unidos 1. Para continuar a nossa compreensão dos mecanismos subjacentes a esta doença enigmática, modelos animais têm sido empregados. Primatas espontaneamente desenvolver endometriose e, portanto, modelos de primatas mais semelhantes ao da doença em mulheres. Modelos de roedores, no entanto, são mais rentáveis ​​e prontamente disponíveis 2. O modelo que descrevemos aqui envolve uma transferência autólogo de tecido uterino ao mesentério intestinal (Figura 1) e foi desenvolvido pela primeira vez no rato 3 e mais tarde transferido para o mouse 4. O objetivo do modelo de roedor autólogo de endometriose cirurgicamente induzida é imitarda doença em mulheres. Nós e os outros já haviam demonstrado que o padrão de expressão alterada do gene observada em lesões de endometriose de camundongos ou ratos espelhos que a observada em mulheres com a doença 5,6. Uma vantagem de realizar a cirurgia em ratos é que a abundância de linhagens de camundongos transgênicos disponíveis podem ajudar os pesquisadores a determinar o papel de componentes específicos importantes no estabelecimento e crescimento da endometriose. Um modelo alternativo no qual excisadas humana fragmentos do endométrio são introduzidos no peritônio de ratos imunocomprometidos também é amplamente utilizada, mas é limitado pela falta de um sistema imunológico normal que é pensado para ser importante na endometriose 2,7. Importante, o modelo do rato da endometriose induzida cirurgicamente é um modelo versátil, que tem sido usado para estudar como o sistema imunológico 8, 9,10 hormônios e fatores ambientais afetam 11,12 endometriose, bem como os efeitos da endometriose sobre fertility 13 e 14 a dor.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Uma descrição da indução cirúrgica de endometriose em camundongos e ratos por auto-transplante de tecido do útero para a cascata arterial do mesentério intestinal.

Modelo do rato de endometriose cirurgicamente induzida pela Auto-transplante de tecido uterino

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

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Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Modelo de Rato OACM Intraluminal: Avaliação infarto cerebral por coloração violeta Cresyl

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

Medicina

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Implantação de Veia Cava Inferior interposição de enxerto no Rato Modelo

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

O transplante de válvula pulmonar Usando um modelo do rato de Heterotópica Transplante de Coração

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Rosa Bengala Fototrombose por imageamento óptico confocal<em&gt; In Vivo</em&gt;: Um Modelo de curso Veículo Individual

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Avaliação da morfológicas e funcionais cardíacas Mudanças no Modelo rato de Transverse aórtica constrição por ecocardiográfica de imagem

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Criação de um modelo de roedor de aneurisma da Aorta Abdominal, bloqueando adventícia Vasa Vasorum perfusão

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

Medições de contratilidade do humano músculo liso uterino para auxílio de desenvolvimento de drogas

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

Um modelo do rato de cirurgia ortopédica para estudar a disfunção cognitiva pós-operatória e regeneração de tecidos

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Aprimoramento do modelo de suínos do miocárdio peito fechado pela padronização do tecido e os procedimentos de recolha de amostras de sangue

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

Medição de contratilidade isométrica da artéria mesentérica Mouse usando fio miografia

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...