Name: mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue
Uploaded: 2012-01-06T12:00:00
Description: Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Chris Kassotis ve bu yazının eleştiri Audrey Bailey ve Dr. Scott Korte, Joseph Beeman, Alison Curfman, Paul Kimball, Bridget Neibreggue, Jacob Redel, Amy Schroder Maija Steinberg, ve Stacey Winkeler optimizasyonu onların yardım için özel bir teşekkür Laboratuvarımızda bu modelin. Finansman Klinik Biodetectives Eğitim Grant (NIH T90) (KEP), Missouri Yaşam Bilimleri Üniversitesi Lisans Araştırma Olanakları Programı, MU Araştırma Konseyi, MU Araştırma Kurulu hibe ve NIH R21HD056441 (SCN) tarafından sağlandı.

1. Canlı hayvan cerrahisi için Planlama <ol><li> Laboratuvar hayvanları ile çalışmak için uygun onayı alındıktan emin olun. </li><li> Sipariş fareler ve yeni bir çevre aklimasyonundan en az bir hafta izin. </li><li> Erkek feromonlar maruz kalma yokluğunda ev sahipliği Dişi fareler bisiklet durdurmak, bir fenomen Whitten etkisi 15,16 olarak anılacaktır. Fareler bisiklet transferi idrarda batırılmış erkek yataklar, her beş günde bir kadın kafes tutmak için. Alternatif olarak, üstü a...

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Farelerde endometriozis cerrahi indüksiyon yaparken dikkat edilmelidir birçok kritik parametre vardır. İlk olarak, endometriozis östrojen bağımlı bir hastalıktır ve sağlam hayvanların veya alternatif östrojenler 20 ile desteklenmiş ovarektomize hayvanlar gibi bu cerrahi olarak yapılmalıdır. İkinci olarak, arteryel kaskad endometriyal biyopsiler dikilmesi çok dikkatli yapılmalıdır. Biz biriyle sadece iki nispeten gevşek knot kullanarak, bağırsak ve sonraki doku nekrozu ve hayvan ölüm...

<table border="1"><tbody><tr><td> Reaktif / ekipman Adı </td><td> Şirket </td><td> Katalog numarası </td></tr><tr><td> Wax kalem </td><td> Balıkçı </td><td> NC9954135 </td></tr><tr><td> Cam slayt </td><td> Balıkçı </td><td> 12-550-433 </td></tr><tr><td> Damlalık </td><td> Balıkçı </td><td> S79383 </td></tr><tr><td> Vajinal sitoloji smear değerlendirilmesi için standart ışık mikroskobu </td><td></td><td></td></tr><tr><td> Buprenorfin HCL c3 (CARJET) 10X1ml </td><td> Butler Hayvan Sağlık Kaynağı </td><td> 022891 </td></tr><tr><td> Steril fosfat tamponlu salin (PBS) </td><td> Gibco </td><td> 14040-117 </td></tr><tr><td> 10.000 U / ml penisilin,% 0.85 NaCl içinde 10.000 mg / ml Streptomisin </td><td> Hyclone </td><td> SV30010 </td></tr><tr><td> İsofluranın </td><td> AbbottHayvan Sağlığı </td><td> 05260-05 </td></tr><tr><td> İsofluranın olmayan solunmasına anestezi sistemi </td><td></td><td></td></tr><tr><td> Devridaim sıcak su ısıtma pedi </td><td></td><td></td></tr><tr><td> 30 ml şırınga kılıf </td><td> Balıkçı </td><td> 14-823-16G </td></tr><tr><td> Toz ücretsiz steril eldiven </td><td> Fisherbrand </td><td> 19020558 </td></tr><tr><td> Oftalmik merhem </td><td> Binbaşı İlaç </td><td> 10033691 </td></tr><tr><td> Küçük elektrik kesme makineleri </td><td> Wahl </td><td> 9861-600 </td></tr><tr><td> Klorheksidin fırçalayın </td><td> Balıkçı </td><td> NC9863042 </td></tr><tr><td> % 70 Etanol </td><td></td><td></td></tr><tr><td> Polylined steril alan </td><td> Busse Hastanesi Tek </td><td> 696 </td></tr><tr><td> Boyut 3 neşter </td><td> Balıkçı </td><td> 22-079-657 </td></tr><tr><td> Number 10 bisturi ağızları </td><td> Balıkçı </td><td> 22-079-681 </td></tr><tr><td> Küçük cerrahi makas </td><td> Roboz </td><td> RS-5850 </td></tr><tr><td> Küçük tırtıklı yarı kavisli bir forseps </td><td> Roboz </td><td> RS-5135 </td></tr><tr><td> 5-0 siyah örgü ipek sütür </td><td> Ethicon </td><td> K870H </td></tr><tr><td> Sterilize pyrex cam Petri kapları </td><td> Corning </td><td> 70160-101 </td></tr><tr><td> 2 mm punch biyopsi </td><td> Miltex </td><td> 33-31 </td></tr><tr><td> Steril gazlı bez </td><td> Kendall </td><td> 1806 </td></tr><tr><td> 6-0 siyah monofilament ethilon naylon sütür </td><td> Ethicon </td><td> 697G </td></tr><tr><td> İğne sürücüleri (isteğe bağlı) </td><td> Dünya Hassas Aletler </td><td> 500023 </td></tr><tr><td> 5-0 boyasız örgülü kaplı vicryl sütür </td><td> Ethicon </td><td> J490G </td></tr><tr><td> 9mm AutoCLIP klipleri yara </td><td> Becton Dickinson </td><td> 427631 </td></tr><tr><td> AutoCLIP uygulayıcısı ve sökücü </td><td> Becton Dickinson </td><td> 427630 </td></tr><tr><td> 23G iğne </td><td> Becton Dickinson </td><td> 305193 </td></tr><tr><td> 1 cc şırınga </td><td> Becton Dickson </td><td> 301025 </td></tr><tr><td> 5X büyüteç standı (isteğe bağlı) </td><td> Balıkçı </td><td> 14-648-23 </td></tr><tr><td> % 10 formalin Tamponlu </td><td> Balıkçı </td><td> SF100-4 </td></tr><tr><td> Kumpaslar </td><td> Roboz </td><td> RS-6466 </td></tr><tr><td> İşleme / gömme kasetleri </td><td> Balıkçı </td><td> 15-197-700A </td></tr><tr><td> Biyopsi köpük pedler </td><td> Balıkçı </td><td> 22-038-222 </td></tr><tr><td> RNAqueous RNA izolasyon kiti </td><td>Ambion </td><td> AM1912 </td></tr><tr><td> Sıvı azot </td><td></td><td></td></tr><tr><td> Snap kap mikrosantrifüj düz üst boru </td><td> Balıkçı </td><td> 02-681-240 </td></tr><tr><td> Ketamin (isteğe bağlı) </td><td> Simga </td><td> K4138 </td></tr><tr><td> Domitor (medetomidine hidroklorür) (isteğe bağlı) </td><td> Tocris </td><td> 2023 </td></tr><tr><td> Antisedan (atipamezole) (isteğe bağlı) </td><td> Sigma </td><td> A9611 </td></tr></tbody></table>

mouse model of surgically-induced endometriosis by auto-transplantation of uterine tissue

Endometriozis, etyolojisi bilinmemektedir, ağrılı, kronik bir hastalık. Ayrıca, tedavi endometriozis lezyonlarının laparoskopik çıkarılması ve / veya kronik ağrı ve kısırlık belirtileri, ilaç yönetimi gerektirebilir. Endometriozis ile ilişkili maliyet Amerika Birleşik Devletleri 1 yılda 22 milyar dolar olarak tahmin edilmiştir. Anlayışımız bu gizemli hastalığın altında yatan mekanizmaları daha için, hayvan modelleri istihdam edilmiştir. Primatlar kendiliğinden endometriozis geliştirmek ve bu nedenle primat modelleri en yakın kadınlarda hastalık benzer. Kemirgen modelleri Ancak, daha uygun maliyetli ve 2 hazır. Burada tarif modeli, rahim dokusunun (Şekil 1) bağırsak mezenter otolog transfer içerir ve ilk 3 sıçan geliştirilen ve daha sonra fare 4 transfer edildi . Amaç, cerrahi ile indüklenen endometriozis otolog kemirgen modeli taklit etmek.kadınlarda hastalık. Biz ve diğerleri daha önce değişmiş gen ekspresyonu desen fare ya da hastalığı 5,6 ile kadınlarda gözlenen sıçanlarda aynalar endometriotik lezyonlar gözlenen olduğunu göstermiştir . Fare ameliyat yerine bir avantajı mevcut transgenik fare suşlarının bolluğu endometriozis kurulması ve büyüme önemli rolü belirli bileşenleri belirleyen araştırmacılar yardımcı olabilir. Eksize insan endometrium parçaları immün sistemi baskılanmış farelerin periton tanıtıldı olduğu alternatif bir model de yaygın olarak kullanılan ancak 2,7 endometriozis önemli olduğu düşünülmektedir normal bir bağışıklık sistemi eksikliği ile sınırlıdır. Daha da önemlisi, cerrahi olarak uyarılan endometriozis fare modelinde, bağışıklık sistemi, 8, 9,10 ve çevresel faktörler 11,12 hormonların yanı sıra endometriozis etkileri Fert üzerinde endometriozis nasıl etkilediğini incelemek için kullanılan bir çok yönlü bir model.ility 13 ve ağrı 14.

Watch this Scientific Journal Video about Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue at JoVE.com

Mouse Model of Surgically-induced Endometriosis by Auto-transplantation of Uterine Tissue

Fare ve sıçanlarda barsak mezenter arter kaskad rahim dokusundaki otomatik nakli endometriozis cerrahi indüksiyon açıklaması.

Uterus Doku Otomatik nakli Cerrahi indüklenen Endometriozis Fare Modeli

Endometriosis is a chronic, painful disease whose etiology remains unknown. Furthermore, treatment of endometriosis can require laparoscopic removal of ...

Research

JoVE Journal

Medicine

Mouse Model of Intraluminal MCAO: Cerebral Infarct Evaluation by Cresyl Violet Staining

Intraluminar MCAO Mouse Model: kresil Violet Boyama tarafından Serebral İnfarktı Değerlendirilmesi

Stroke is the third  cause of mortality and the leading cause of disability in the World. Ischemic  stroke accounts for approximately 80% of all strokes. ...

Implantation of Inferior Vena Cava Interposition Graft in Mouse Model

Fare Modeli Inferior Vena Cava İnterpozisyon Greft İmplantasyonu

Biodegradable scaffolds seeded with bone marrow mononuclear cells (BMCs) are often used for reconstructive surgery to treat congenital cardiac anomalies. ...

Transplantation of Pulmonary Valve Using a Mouse Model of Heterotopic Heart Transplantation

Heterotopik Kalp Nakli bir Fare Modeli Kullanarak Pulmoner Vana Nakli

Tissue engineered heart valves, especially decellularized valves, are starting to gain momentum in clinical use of reconstructive surgery with mixed ...

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke

Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo:  A Model of Single Vessel Stroke

Konfokal Optik Görüntüleme ile Rose Bengal Fototromboz<em&gt; In Vivo</em&gt;: Tek Gemi İnme Bir Model

In vivo imaging techniques have increased in utilization due to recent advances in imaging dyes and optical technologies, allowing for the ability to ...

Assessment of Cardiac Morphological and Functional Changes in Mouse Model of Transverse Aortic Constriction by Echocardiographic Imaging

Ekokardiyografik Imaging tarafından Enine Aort Konstrüksiyon Mouse Modeli Kardiyak Morfolojik Değerlendirilmesi ve Fonksiyonel Değişiklikler

Transverse aortic constriction (TAC) in mice has been used as a valuable model to study mechanisms of cardiac hypertrophy and heart failure1. A reliable ...

Creation of a Rodent Model of Abdominal Aortic Aneurysm by Blocking Adventitial Vasa Vasorum Perfusion

Abdominal aort anevrizması Adventitial Vasa Vasorum perfüzyon engelleyerek kemirgen bir Model oluşturulması

The adventitial vasa vasorum (VV) provides oxygen and nourishment to the aortic wall. Hypoxia in the aortic wall can cause enlarged abdominal aortic ...

Contractility Measurements of Human Uterine Smooth Muscle to Aid Drug Development

İnsan rahim düz kas yardım ilaç geliştirme için contractility ölçümleri

Discovery and characterization of novel pharmaceutical compounds or biochemical probes rely on robust and physiologically relevant assay systems. We ...

A Mouse Model of Orthopedic Surgery to Study Postoperative Cognitive Dysfunction and Tissue Regeneration

Ameliyat sonrası bilişsel işlev bozukluğu ve doku rejenerasyonu çalışmaya ortopedik cerrahi bir fare modeli

Surgery is commonly used to improve and maintain quality of life. Unfortunately, in vulnerable patients such as the elderly, complications may occur and ...

Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures

Kapalı göğüs domuz miyokard infarktüsü manken standardizasyon doku ve kan örnekleme prosedürleri tarafından geliştirilmesi

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved ...

Isometric Contractility Measurement of the Mouse Mesenteric Artery Using Wire Myography

İzometrik Contractility ölçüm tel Myography kullanarak fare mezenterik arter

The wire myograph technique is used to assess the contractility of vascular smooth muscles in response to depolarization, GPCR agonists/inhibitors and ...