Name: formulation of diblock polymeric nanoparticles through nanoprecipitation technique
Uploaded: 2011-09-20T13:00:00
Description: Watch this Scientific Journal Video about Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique at JoVE.com

这项工作是由对抗癌症，卡罗来纳州为纳米技术卓越试点补助，大学癌症研究基金会和国家卫生研究所的K - 12的职业发展奖中心的高尔夫球手。

1。 PLGA - B - PEG聚合物的合成<ol><li>聚（D，L -乳酸- CO -乙醇酸）（PLGA）终端羧基 - 羧酸（PLGA）是溶解在浓度为5mm的PLGA（材料一节中提到的）在任何溶剂。 PLGA可以在这个浓度轻轻搅拌溶解。 </li><li>国民保健服务（分子量115.09）和EDC（分子量191.7）溶解在PLGA溶液浓度为25mm。 （EDC和NHS在添加了5次计量过剩相比，以PLGA）。 PLGA -羧酸转化为PLGA - NHS轻轻搅拌约1小时的PLGA - 羧酸溶液加入EDC和NHS。 </li><...

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nanoprecipitation方法使用嵌段共聚物是一种简单，快速的方法，工程师聚合物纳米粒子。由此产生的纳米粒子组成一个疏水核心，可用于难溶化合物的交付使用。表面的亲水层，使优良的水溶性，同时提供了可能进一步靶向配体共轭基团。 有许多纳米颗粒平台，包括脂质体，聚合物纳米粒子，树枝状，金属粒子，量子点14。在这些平台中，聚合物纳米粒子的平台是一个?...

<table border="1"><tbody><tr><td> 试剂 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td>东区区议会</td><td> Thermo Scientific的</td><td> 22980 </td><td>共轭试剂</td></tr><tr><td> NHS的</td><td> Thermo Scientific的</td><td> 24500 </td><td>共轭试剂</td></tr><tr><td>胺聚乙二醇 - 羧酸</td><td> Laysan生物公司</td><td> NH2 - PEG - CM - 5000 </td><td>聚合物（可以使用任何的PEG兆瓦，5000这里列出） </td></tr><tr><td> PLGA - carbxylate </td><td> Lactel </td><td> B6013 - 2 </td><td>聚合物</td></tr><tr><td>二氯甲烷（DCM） </td><td> Sigma - Aldrich公司</td><td> 34856 </td><td>溶剂</td></tr><tr><td>乙腈&gt;纯度达99％ </td><td> Sigma - Aldrich公司</td><td> 34851 </td><td>溶剂</td></tr><tr><td>甲醇&gt;纯度达99％ </td><td> Sigma - Aldrich公司</td><td> 34860 </td><td>洗</td></tr></tbody></table>

formulation of diblock polymeric nanoparticles through nanoprecipitation technique

纳米技术是一项相对较新的科学的分支，它涉及到利用规模的纳米颗粒（纳米颗粒）的独特性能。纳米粒子可以被设计在一个精确的时尚，他们的规模，组成和表面化学可仔细控制。这使前所未有的自由，他们的货物的基本特性，如溶解度，扩散，生物分布，释放特性和免疫原性，修改一些。自创建以来，已经利用纳米粒子科学和医学的许多领域，包括药物递送，成像和细胞生物学 1-4 。但是，它并没有得到充分利用“纳米技术实验室”由于感知技术壁垒之外。在这篇文章中，我们描述了一个简单的方法合成的聚合物为基础的纳米颗粒平台，具有广泛的应用潜力。 第一步是合成一个嵌段共聚物，既是一个疏水区和亲水域。使用模型聚合物PLGA和PEG，我们描述了共轭反应，使用EDC / NHS的化学 5（图1） 。我们还讨论了聚合物的净化过程。合成的嵌段共聚物可以自组装在nanoprecipitation过程中通过疏水 - 亲水相互作用成​​纳米粒子。 所描述的聚合物纳米粒子是非常灵活。可以利用纳米颗粒的疏水核心，进行药物输送experiments6难溶性药物。此外，纳米粒子可以克服难溶性的分子生物学试剂，如渥曼青霉素，这就需要一个像DMSO溶剂，有毒溶剂的问题。然而，二甲基亚砜细胞毒性，干扰实验。这些难溶性药物和试剂，可以有效地交付使用聚合物纳米粒子的毒性极小。高分子纳米粒子也可以被载入与荧光染料和细胞内贩卖的研究利用。最后，这些聚合物纳米粒子可以通过表面的PEG目标配体的结合。这些有针对性的纳米粒子，可以利用标签或细胞中的7-10的特定抗原决定簇。

Watch this Scientific Journal Video about Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique at JoVE.com

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

本文介绍了nanoprecipitation方法使用嵌段共聚物的合成聚合物为基础的纳米粒子。我们将讨论合成的嵌段共聚物，nanoprecipitation技术，和潜在的应用。

制定通过Nanoprecipitation技术嵌段聚合物纳米粒子

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Research

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科研

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