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DOI :
10.3791/3410-v
January 27th, 2012
Chapters
0:05
Title
2:19
Introducing the Probe to Cells
3:27
Monitoring Ratiometric FRET
6:12
Verifying and Optimizing Conditions
6:43
Analyzing FRET
11:24
Representative Imaging Results
12:52
Conclusion
FRETに基づく記者はますます、生細胞におけるキナーゼとホスファターゼの活動を監視するために使用されています。ここでは、ターゲットのリン酸化の細胞周期依存性の変化を評価するためのFRETに基づくレポーターを使用する方法の方法を説明します。
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