Name: herbivore-induced blueberry volatiles and intra-plant signaling
Uploaded: 2011-12-18T14:00:00
Description: Watch this Scientific Journal Video about Herbivore-induced Blueberry Volatiles and Intra-plant Signaling at JoVE.com

Forfatteren takker Robert Holdcraft til teknisk bistand. Denne undersøgelse blev finansieret delvist af en USDA CSREES særligt tilskud (2009-34155-19957) og luge midler (NJ08192).

1. Lokale induktion af flygtige stoffer: planteæder skader <ol><li> To grene af blåbær planter er i sække med en spundet polyester ærme. </li><li> Seks sigøjner-møl larver (2 nd -3 rd instars) er placeret inde i poser og lov til at leve af planter i 2 dage før flygtige kollektion. Kontrol planterne ikke modtager nogen larver. </li><li> Flygtige emissioner er indsamlet som beskrevet nedenfor (protokol # 7) på dag 3 (Figur 1). Polyester ærmer forblive på planterne for at...

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	<li>Dicke, M., Van Loon, J. J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multitrophic+effects+of+herbivore-induced+plant+volatiles+in+an+evolutionary+context.">Multitrophic effects of herbivore-induced plant volatiles in an evolutionary context.</a> Entomol. Exp. Appl. 97, 237-237 (2000).</li><li>Mumm, R., Dicke, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Variation+in+natural+plant+products+and+the+attraction+of+bodyguards+involved+in+indirect+plant+defense.">Variation in natural plant products and the attraction of bodyguards involved in indirect plant defense.</a> Can. J. Zool. 88, 628-628 (2010).</li><li>Hopke, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Herbivore-induced+volatiles:+the+emission+of+acyclic+homoterpenes+from+leaves+of+Phaseolus+lunatus+and+Zea+mays+can+be+triggered+by+a+&#946;-glucosidase+and+jasmonic+acid.">Herbivore-induced volatiles: the emission of acyclic homoterpenes from leaves of Phaseolus lunatus and Zea mays can be triggered by a &#946;-glucosidase and jasmonic acid.</a> FEBS Lett. 352, 146-146 (1994).</li><li>Rodriguez-Saona, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+and+electrophysiological+responses+of+the+emerald+ash+borer,+Agrilus+planipennis,+to+induced+volatiles+of+Manchurian+ash,+Fraxinus+mandshurica.">Behavioral and electrophysiological responses of the emerald ash borer, Agrilus planipennis, to induced volatiles of Manchurian ash, Fraxinus mandshurica.</a> Chemoecology. 16, 75-75 (2006).</li><li>Rodriguez-Saona, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Herbivore+induced+volatiles+in+the+perennial+shrub,+Vaccinium+corymbosum,+and+their+role+in+inter-branch+signaling.">Herbivore induced volatiles in the perennial shrub, Vaccinium corymbosum, and their role in inter-branch signaling.</a> J. Chem. Ecol. 35, 163-163 (2009).</li><li>Karban, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Damage-induced+resistance+in+sagebrush:+volatiles+are+key+to+intra-+and+interplant+communication.">Damage-induced resistance in sagebrush: volatiles are key to intra- and interplant communication.</a> Ecology. 87, 922-922 (2006).</li><li>Frost, C. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Within-plant+signalling+by+volatiles+overcomes+vascular+constraints+on+systemic+signalling+and+primes+responses+against+herbivores.">Within-plant signalling by volatiles overcomes vascular constraints on systemic signalling and primes responses against herbivores.</a> Ecol. Lett. 10, 490-490 (2007).</li><li>Heil, M., Bueno Silva, J. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Within-plant+signaling+by+volatiles+leads+to+induction+and+priming+of+an+indirect+plant+defense+in+nature.">Within-plant signaling by volatiles leads to induction and priming of an indirect plant defense in nature.</a> Proc. Natl. Acad. Sci. U.S.A. 104, 5467-5467 (2007).</li><li>Turlings, T. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exploitation+of+herbivore-induced+plant+odors+by+host-seeking+parasitic+wasps.">Exploitation of herbivore-induced plant odors by host-seeking parasitic wasps.</a> Science. 250, 1251-1251 (1990).</li><li>Makovic, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Volatiles+involved+in+the+nonhost+rejection+of+Fraxinus+pennsylvanica+by+Lymantria+dispar+larvae.">Volatiles involved in the nonhost rejection of Fraxinus pennsylvanica by Lymantria dispar larvae.</a> J. Agric. Food Chem. 44, 929-929 (1996).</li></ol>

Push-pull flygtige samling apparatur beskrevet her repræsenterer en standardmetode for headspace samlinger af plante-flygtige stoffer. Dette apparat blev brugt til at bestemme flygtige reaktion blåbær blade til herbivory af sigøjner møl, larver og også givet mig mulighed for at levere nye beviser for den rolle, HIPVs i samhandelen inden for plante signalering. Resultaterne præsenteres her viser, at Caterpillar fodring, eksogent-anvendte MeJA, og mekaniske sårede øget flygtige emissi...

<table border="1"><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalog nummer </td><td> Kommentarer </td></tr><tr><td> Flygtige kollektion kamre </td><td> Analytisk Research Systems, Inc. </td><td> VCC-G6X12DT-1P </td><td> Gainesville, FL </td></tr><tr><td> Luftkompressor, 20 gal, oliefri, 2 hk </td><td> Westward </td><td> 3JR71 </td><td> Solgt af Grainger, Inc. </td></tr><tr><td> Air levering system </td><td> Analytisk Research Systems, Inc. </td><td> VCS-ADS-4AFM4C </td><td> Gainesville, FL </td></tr><tr><td> Air indsamlingssystem </td><td> Analytisk Research Systems, Inc. </td><td> VCS-MVCS-4CX1P </td><td> Gainesville, FL </td></tr><tr><td> Vakuumpumpe 100-150V, ¼ hk </td><td> Gast Manufacturing, Inc. </td><td> <a href="http://www.grainger.com/Grainger/GAST-Vacuum-Pump-4F740">4F740</a> </td><td> Solgt af Grainger, Inc. </td></tr><tr><td> Methyl jasmonate </td><td> Sigma-Aldrich &lt;/ Td&gt; <td> J2500 </td><td> St. Louis, MO </td></tr><tr><td> Tween-20 </td><td> Sigma-Aldrich </td><td> 93773 </td><td> St. Louis, MO </td></tr><tr><td> Rhodamine-B </td><td> Sigma-Aldrich </td><td></td><td> St. Louis, MO </td></tr><tr><td> Plastik spray flasker, 2 ounce </td><td> Setco Inc. </td><td></td><td> Cranbury, NJ </td></tr><tr><td> Spun polyester ærmer </td><td> Rockingham Muligheder Corp </td><td></td><td> Reidsville, NC </td></tr><tr><td> Super-Q flygtige samling fælder </td><td> Analytisk Research Systems, Inc. </td><td> VCT-1/4X3-SPQ </td><td> Gainesville, FL </td></tr><tr><td> Scion Image Software </td><td> Scion Corporation </td><td></td><td> Frederick, MD </td></tr><tr><td> Dichlormethan </td><td> Sigma-Aldrich </td><td> 270997 </td><td> St. Louis, MO </td></tr><tr><td> Gaskromatograf HP 6890 </td><td> Hewlett Packard </td><td></td><td></td></tr><tr><td> Gaskromatograf Varian 3400 </td><td> Varian </td><td&gt; </td><td></td></tr><tr><td> n-oktan </td><td> Sigma-Aldrich </td><td> 296988 </td><td> St. Louis, MO </td></tr><tr><td> Massespektrometer MAT 8230 </td><td> Finnigan </td><td></td><td> San Jose, Californien </td></tr><tr><td> HP-1 GC kolonne </td><td> Agilent Technologies </td><td></td><td> Palo Alto, CA </td></tr><tr><td> MDN-5S GC kolonne </td><td> Supelco, Inc. </td><td></td><td> Bellefonte, PA </td></tr></table>

herbivore-induced blueberry volatiles and intra-plant signaling

Planteæder-induceret plante flygtige (HIPVs) er almindeligt udsendes fra anlæg efter planteæder angreb 1,2. Disse HIPVs er primært reguleret af den defensive planten hormon jasmonic syre (JA) og flygtige afledte methyl jasmonate (MeJA) 3,4,5. I løbet af de sidste 3 årtier forskere har dokumenteret, at HIPVs kan afvise eller tiltrække planteædere, tiltrække de naturlige fjender planteædere, og i nogle tilfælde kan fremkalde eller prime plante forsvar forud for planteæder angreb. I et nyligt papir 6, rapporterede jeg, at fodring af sigøjner møl larver, eksogene MeJA ansøgning, og mekaniske skader inducere emissioner af flygtige stoffer fra blåbær planter, men anderledes. Hertil kommer, reagere blåbær grene til HIPVs udsendes fra nabolandet grene af den samme plante ved at øge niveauet af JA og modstand mod planteædere (dvs. direkte plante forsvar), og ved priming flygtige emissioner (dvs. indirekte plante forsvar). Tilsvarende findersomheder er blevet rapporteret for nylig for bynke 7, poppel 8 og lima bønner 9 .. Her vil jeg beskrive en push-pull-metoden til indsamling af blåbær flygtige induceret af planteædende (sigøjner-møl) fodring, udefrakommende MeJA program og mekaniske skader. De flygtige kollektionen består af en 4 L flygtige kollektion kammer, en 2-piece guillotine, en luft delivery system, der renser indkommende luft, og et vakuum-system er tilsluttet en fælde fyldt med Super-Q adsorbent til at indsamle flygtige 5,6,10 . Flygtige stoffer indsamlet i Super-Q fælder elueres med dichlormethan og derefter adskilles og kvantificeres ved hjælp af gaskromatografi (GC). Dette flygtige samling metode blev anvendt n mit studie 6 for at undersøge de volatile reaktion ubeskadiget grene af eksponering for flygtige stoffer fra planteædere-beskadigede grene inden blåbær planter. Disse metoder er beskrevet her. Kort fortalt, er ubeskadigede blåbær grene udsat for HIPVs tilbagem nabo grene inden for den samme plante. Ved hjælp af samme teknik som beskrevet ovenfor, er flygtige stoffer afgives fra grene efter udsættelse for HIPVs indsamlet og analyseret.

Watch this Scientific Journal Video about Herbivore-induced Blueberry Volatiles and Intra-plant Signaling at JoVE.com

Herbivore-induced Blueberry Volatiles and Intra-plant Signaling

En push-pull-metoden til indsamling af anlægget flygtige stoffer er beskrevet. Metoden giver mulighed for en sammenligning af flygtige stoffer som følge af planteædende fodring, eksogene methyl jasmonate, og mekaniske skader. Denne teknik er også brugt til at undersøge flygtige reaktion ubeskadiget grene af eksponering for flygtige stoffer fra planteædere-beskadigede grene inden blåbær planter.

Planteæder-induceret Blueberry Flygtige stoffer og intra-anlæg Signaling

Herbivore-induced plant volatiles (HIPVs) are commonly emitted from plants after herbivore attack1,2.  These HIPVs are mainly regulated by the defensive ...

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