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DOI :
10.3791/3448-v
•
10:58 min
February 27th, 2012
Chapters
0:05
Title
1:33
Day 1, Preparation of 96-well NGM RNAi Plates, 96-DeppWell LB Plates, and RNAi Bacterial Clone Culturing
3:14
Day 2, Seeding RNAi Bacterial Clones onto 96-well NGM RNAi Plates
4:46
Days 3 and 4, RNAi Feeding of Worms and Infection of Worms with D. coniospora Spores
6:02
Day 5, Observation and Storage of Plates Prior to Automated Quantitative Analysis
7:29
Representative RNAi Results
10:27
Conclusion
我々は、使用してプロトコルを記述する C.エレガンスとRNAiはそのような集団内の個々のワームの蛍光、サイズや不透明度などの複数のパラメータの自動測定を可能にするライブラリを供給する。私達は中の抗真菌自然免疫に関与する遺伝子を識別するために画面の一例を与える C.エレガンス。
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