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DOI :
10.3791/3453-v
February 16th, 2012
Chapters
0:05
Title
1:39
Preparation of the Electrostatic Chamber
2:54
Neural Progenitor Cell Isolation
4:09
Application of the Electric Field to the Electrostatic Chamber
7:18
Preparation of the Organotypic Spinal Cord Slice
8:40
Injection of Hoechst 33342 Labeled Neural Progenitor Cells into the Organotypic Spinal Cord Slice
9:38
Results: Neural Progenitor Cells Show Directed Migration in the Electric Field and the Organotypic Spinal Cord Slice
10:46
Conclusion
このプロトコルは、細胞を追跡することができますカスタム設計electrotactic室で2Dと3D環境を確立するために使用されるメソッドを示しています生体/ ex vivoでの直流(DC)電界(EFS)を指示する走電性および他の細胞応答を調べるために、単一細胞レベルでの経時的記録を使用します。
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