Name: presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry
Uploaded: 2012-01-12T13:00:00
Description: Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

韦恩州立大学国家酒精滥用和酒精中毒研究所（NIAAA; AA - 016967和AA016967 - 01S1;谭）提供的资金，启动资金，和韦恩州立大学研究资助计划。内容完全是作者的责任，并不代表NIAAA或国立卫生研究院的官方意见。

1。实验要领电极的制作<ul type="circle"><li>有无数的碳纤维微电极的制备方法，因为大多数是在内部。通常是什么决定了电极制造细节的电化学技术应用于电极（如安培与FSCV）。对于FSCV，微电极可以在内部使用以下三个步骤的过程。对于碳纤维电极的制作更完整的描述，可以看到最近的朱庇特的第1条。但是请注意，下面描述的电极是圆柱形的碳纤维微电极，它需要较?...

<ol>
	<li>Pike, C. M., Grabner, C. P., Harkins, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fabrication+of+Amperometric+Electrodes.">Fabrication of Amperometric Electrodes.</a> J. Vis. Exp. (27), e1040-e1040 (2009).</li><li>Lack, A. K., Diaz, M. R., Chappell, A., DuBois, D. W., McCool, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+ethanol+and+withdrawal+differentially+modulate+pre-+and+postsynaptic+function+at+glutamatergic+synapses+in+rat+basolateral+amygdala.">Chronic ethanol and withdrawal differentially modulate pre- and postsynaptic function at glutamatergic synapses in rat basolateral amygdala.</a> J. Neuropyhysiol. 98, 3185-3196 (2007).</li><li>John, C. E., Jones, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voltammetric+characterization+of+the+effect+of+monoamine+uptake+inhibitors+and+release+on+dopamine+and+serotonin+uptake+in+mouse+caudate-putamen+and+substantia+nigra+slices.">Voltammetric characterization of the effect of monoamine uptake inhibitors and release on dopamine and serotonin uptake in mouse caudate-putamen and substantia nigra slices.</a> Neuropharmacology. 52, 1596-1605 (2007).</li><li>Wightman, R. M., Amatore, C., Engstrom, R. C., Hale, P. D., Kistensen, E. W., Kuhr, W. G., May, L. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+characterization+of+dopamine+overflow+and+uptake+in+the+rat+striatum.">Real-time characterization of dopamine overflow and uptake in the rat striatum.</a> Neuroscience. 25, 513-523 (1988).</li><li>Wightman, R. M., Zimmerman, J. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Control+of+dopamine+extracellular+concentration+in+rat+striatum+by+impulse+flow+and+uptake.">Control of dopamine extracellular concentration in rat striatum by impulse flow and uptake.</a> Brain. Res. Brain. Res. Rev. 15, 135-144 (1990).</li><li>Jones, S. R., Garris, P. A., Kilts, C. D., Wightman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comparison+of+dopamine+uptake+in+the+basolateral+amygdaloid+nucleus,+caudate-putamen,+and+nucleus+accumbens+of+the+rat.">Comparison of dopamine uptake in the basolateral amygdaloid nucleus, caudate-putamen, and nucleus accumbens of the rat.</a> J. Neurochem. 64, 2581-2589 (1995).</li><li>Michael, D. J., Wightman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrochemical+monitoring+of+biogenic+amine+neurotransmission+in+real+time.">Electrochemical monitoring of biogenic amine neurotransmission in real time.</a> J. Pharm. Biomed. Anal. 19, 33-46 (1999).</li><li>Pajski, M. L., Venton, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adenosine+release+evoked+by+short+electrical+stimulations+in+striatal+brain+slices+is+primarily+activity+dependent.">Adenosine release evoked by short electrical stimulations in striatal brain slices is primarily activity dependent.</a> A.C.S. Chem. Neurosci. 1, 775-787 (2010).</li><li>Sanford, A. L., Morton, S. W., Whitehouse, K. L., Oara, H. M., Lugo-Morales, L. Z., Roberts, J. G., Sombers, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voltammetric+detection+of+hydrogen+peroxide+at+carbon+fiber+microelectrodes.">Voltammetric detection of hydrogen peroxide at carbon fiber microelectrodes.</a> Anal. Chem. 82, 5205-5210 (2010).</li><li>Park, J., Kile, B. M., Wightman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+voltammetric+monitoring+of+norepinephrine+release+in+the+rat+ventral+bed+nucleus+of+the+stria+terminalis+and+anteroventral+thalamic+nucleus.">In vivo voltammetric monitoring of norepinephrine release in the rat ventral bed nucleus of the stria terminalis and anteroventral thalamic nucleus.</a> Eur. J. Neurosci. 30, 2121-2133 (2009).</li><li>Hashemi, P., Dankoski, E. C., Petrovic, J., Keithley, R. B., Wightman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Voltammetric+detection+of+5-hydroxytryptamine+release+in+the+rat+brain.">Voltammetric detection of 5-hydroxytryptamine release in the rat brain.</a> Anal. Chem. 81, 9462-9471 (2009).</li><li>Borue, X., Cooper, S., Hirsh, J., Condron, B., Venton, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+evaluation+of+serotonin+release+and+clearnece+in+drosophila.">Quantitative evaluation of serotonin release and clearnece in drosophila.</a> J. Neuroscience. Methods. 179, 300-308 (2009).</li><li>Vickrey, T. L., Condron, B., Venton, B. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+endogenous+dopamine+changes+in+drosophila+melanogaster+using+fast-scan+cyclic+voltammetry.">Detection of endogenous dopamine changes in drosophila melanogaster using fast-scan cyclic voltammetry.</a> Anal. Chem. 81, 9306-9313 (2009).</li><li>Makos, M. A., Han, K. A., Heien, M. L., Ewing, A. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Using+in+vivo+electrochemistry+to+study+the+physiological+effects+of+cocaine+and+other+stimulants+on+the+drosophila+melanogaster+dopamine+transporter.">Using in vivo electrochemistry to study the physiological effects of cocaine and other stimulants on the drosophila melanogaster dopamine transporter.</a> A.C.S. Chem Neurosci. 1, 74-83 (2009).</li><li>Budygin, E. A., John, C. E., Mateo, Y., Daunais, J. B., Friedman, D. P., Grant, K. A., Jones, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chronic+ethanol+exposure+alters+presynaptic+dopamine+function+in+striatum+of+monkeys:+a+preliminary+study.">Chronic ethanol exposure alters presynaptic dopamine function in striatum of monkeys: a preliminary study.</a> Synapse. 50, 266-268 (2003).</li><li>Jones, S. R., Gainetdinov, R. R., Jaber, M., Giros, B., Wightman, R. M., Caron, M. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Profound+neuronal+plasticity+in+response+to+inactivation+of+the+dopamine+transporter.">Profound neuronal plasticity in response to inactivation of the dopamine transporter.</a> PNAS. 95, 4029-4034 (1998).</li><li>Kennedy, R. T., Jones, S. R., Wightman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dynamic+observation+of+dopamine+autoreceptor+effects+in+rat+striatal+slices.">Dynamic observation of dopamine autoreceptor effects in rat striatal slices.</a> J. Neurochem. 59, 449-445 (1992).</li><li>Rice, M. E., Cragg, S. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nicotine+amplifies+reward-related+dopamine+signals+in+striatum.">Nicotine amplifies reward-related dopamine signals in striatum.</a> Nat. Neurosci. 7, 583-584 (2004).</li><li>Zhang, L., Doyon, W. M., Clark, J. J., Phillips, P. E., Dani, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Controls+of+tonic+and+phasic+dopamine+transmission+in+the+dorsal+and+ventral.">Controls of tonic and phasic dopamine transmission in the dorsal and ventral.</a> 76, 396-404 (2009).</li><li>Paredes, D., Grnaholm, A. C., Bickford, P. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+NGF+and+BDNF+on+baseline+glutamate+and+dopamine+release+in+the+hippocampal+formation+of+the+adult+rat.">Effects of NGF and BDNF on baseline glutamate and dopamine release in the hippocampal formation of the adult rat.</a> Brain. Res. 11141, 56-64 (2007).</li><li>Goggi, J., Puller, I. A., Carney, S. L., Bradford, H. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Signalling+pathways+involved+in+the+short-term+potentiation+of+dopamine+release+by+BDNF.">Signalling pathways involved in the short-term potentiation of dopamine release by BDNF.</a> Brain. Res. 968, 156-161 (2003).</li><li>Cheer, J. F., Wassum, K. M., Heien, M. L., Phillips, P. E., Wightman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cannabinoids+enhance+subsecond+doapmine+relese+in+the+nucleus+accumbens+of+awake+rats.">Cannabinoids enhance subsecond doapmine relese in the nucleus accumbens of awake rats.</a> J. Neurosci. 24, 4393-4400 (2004).</li><li>Phillips, P. E., Stuber, G. D., Heien, M. L., Wightman, R. M., Carelli, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Subsecond+dopamine+release+promotes+cocaine+seeking.">Subsecond dopamine release promotes cocaine seeking.</a> Nature. 422, 614-618 (2003).</li><li>Robinson, D. L., Heien, M. L., Wightman, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Frequency+of+dopamine+concentration+transients+increases+in+dorsal+and+ventral+striatum+of+male+rats+duing+introduction+of+conspecifics.">Frequency of dopamine concentration transients increases in dorsal and ventral striatum of male rats duing introduction of conspecifics.</a> J. Neurosci. 22, 10477-10486 (2002).</li></ol>

这里介绍的协议，演示了如何准备和使用FSCV实验小鼠冠状脑切片。虽然这种方法是具体 ​​的获取和测量多巴胺的动态，其他神经递质如腺苷，过氧化氢 ​​，去甲肾上腺素和血清素已在体内或体外监测与FSCV 3，8 - 11 。 FSCV可以用来监视其他神经化学物质的一些简单的修改适用于工作电极3，11的波形。由于这些神经化学物种中，有很多类似的氧化电位，产生的循环伏安每...

<table border="1"><tbody><tr><td> 名称 </td><td> 公司 </td><td> CAS号码 </td></tr><tr><td width="274"> 试剂 </td><td></td><td></td></tr><tr><td width="274">氯化钾</td><td>费舍尔</td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274">氯化钠</td><td> EMD化学品</td><td width="142" valign="top"> 764714​​5 </td></tr><tr><td width="274">氯化镁</td><td width="194" valign="top">费舍尔</td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274">氯化钙</td><td width="194" valign="top">费舍尔</td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274">碳酸氢钠</td><td width="194" valign="top"> EMD化学品</td><td width="142" valign="top"> 144558 </td></tr><tr><td width="274">磷酸钠，二元</td><td width="194" valign="top">电解二氧化锰化学小号</td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D -葡萄糖</td><td width="194" valign="top">费舍尔</td><td width="142" valign="top"> 50997 </td></tr><tr><td width="274">抗坏血酸</td><td width="194" valign="top">费舍尔</td><td width="142" valign="top"> 50817 </td></tr><tr><td width="274">蔗糖</td><td width="194" valign="top">费舍尔</td><td> 57501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> 材料 </td><td width="194" valign="top"> 供应商 </td><td> 目录编号 </td></tr><tr><td width="274">碳纤维</td><td>古德费洛Oakdale，PA </td><td></td></tr><tr><td width="274">玻璃毛细管</td><td>上午系统Carlsborg WA。 </td><td> 602000 </td></tr><tr><td width="274">银线</td><td> A -M系统Carlsborg西澳。 </td><td> 787000 </td></tr><tr><td width="274">钨刺激​​电极</td><td>塑料之一，弗吉尼亚州罗阿诺克</td><td></td></tr><tr><td width="274">铂丝</td><td></td><td></td></tr><tr><td width="274">导线</td><td>斯夸尔斯电子，科尼利厄斯，或</td><td></td></tr><tr><td width="274">乐泰404瞬间粘合剂</td><td> Hankal公司洛基山 CT检查。 </td><td></td></tr><tr><td width="274">刀片</td><td>世界精密仪器公司的佛罗里达州。 </td><td></td></tr><tr><td width="274"> BD脊髓针</td><td> BD医疗系统， 位于美国新泽西州Franlin湖， </td><td>楼盘405234 </td></tr><tr><td width="274">手术刀片</td><td>羽毛安全剃刀有限公司。日本</td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> 软件 </td><td width="194" valiGN =“顶”&gt; 供应商 </td><td> 目录编号 </td></tr><tr><td width="274"> TH软件</td><td>欧空局的公司，切姆斯福德，MA </td><td></td></tr><tr><td width="274"> 仪器 </td><td width="194" valign="top"> 供应商 </td><td> 目录编号 </td></tr><tr><td width="274">浸没录音室</td><td>自定义科学，丹佛，CO </td><td></td></tr><tr><td width="274"> Neorolog刺激隔离器</td><td>英国赫特福德郡，Digitmeter， </td><td></td></tr><tr><td width="274">自动温度控制器</td><td>华纳仪器公司</td><td></td></tr><tr><td width="274">显微镜（SZX7） </td><td>奥林巴斯</td><td></td></tr><tr><td width="274">显微镜</td><td>费舍尔</td><td></td></tr><tr><td width="274"> 3000 Vibratome切片系统</td><td>圣楼是，莫。 </td><td></td></tr><tr><td width="274">灌注泵</td><td>沃森马洛有限公司，康沃尔郡法尔茅斯 - 英格兰</td><td> H110708 </td></tr><tr><td width="274">微管拉拔</td><td> Narishige，东京，日本</td><td></td></tr><tr><td width="274"> ChemClamp电位</td><td>大矸法团， 明尼苏达州明尼阿波利斯。 </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

广泛的研究主要集中于神经递质多巴胺，因为其在滥用药物（如可卡因和安非他明），发挥的作用，在精神病（如精神分裂症，多动症），和其在退行性参与的行动机制的重要性障碍像帕金森氏症和亨廷顿氏病。在正常生理条件下，多巴胺是众所周知的规范自发活动，认知，学习，情感的影响，和神经内分泌激素的分泌。多巴胺神经元密度最大的一个是纹状体内，可分为两个不同的神经解剖区域，被称为伏隔核和尾壳。目的是要说明的小鼠纹状体内一般为片快速扫描循环伏安（FSCV）协议。 FSCV是一个定义良好的电化学技术在D实时提供机会来衡量多巴胺的释放和吸收iscrete大脑区域。在FSCV碳纤维微电极（直径〜7微米）用于检测多巴胺的氧化。使用FSCV检测多巴胺的分析优势，是100毫秒和空间分辨率小于10微米在体内微透析提供的补充信息，增强的时间分辨率。

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

使用快速扫描循环伏安法测量纹状体脑片电刺激突触前多巴胺动态。

突触前多巴胺纹状体脑片的快速扫描循环伏安动态

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

Research

JoVE Journal

Neuroscience

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Vibrodissociation从啮齿动物脑切片的神经元，研究突触传递和图像突触前的码头

Mechanical dissociation of neurons from the central nervous system has the advantage that presynaptic boutons remain attached to the isolated neuron of ...

科研

神经科学

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

器官型培养的神经元雪崩多电极阵列记录

The cortex is spontaneously active, even in the absence of any particular input or motor output.  During development, this activity is important for the ...

Voltage Biasing, Cyclic Voltammetry, &amp; Electrical Impedance Spectroscopy for Neural Interfaces

Voltage Biasing, Cyclic Voltammetry, & Electrical Impedance Spectroscopy for Neural Interfaces

偏置电压，循环伏安，神经接口电阻抗光谱

Electrical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measure properties of the electrode-tissue interface without additional invasive ...

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

在器官型海马脑片搭配全细胞记录

Pair recordings involve simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling not only direct ...

Whole-cell Patch-clamp Recordings in Brain Slices

在脑片全细胞膜片钳记录

Whole-cell patch-clamp recording is an electrophysiological technique that allows the study of the&#160;electrical properties of a substantial part of the ...

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0

使用QNsim1.0从电刺激多巴胺神经传递数据建模快速扫描循环伏安数据

Central dopaminergic (DAergic) pathways have an important role in a wide range of functions, such as attention, motivation, and movement. Dopamine (DA) is ...

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

海马鼠脑切片的突触密度评价

In the brain, synapses are specialized junctions between neurons, determining the strength and spread of neuronal signaling. The number of synapses is ...

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

脑切片神经元树突的双光子钙成像

Calcium (Ca2+) imaging is a powerful tool to investigate the spatiotemporal dynamics of intracellular Ca2+ signals in neuronal dendrites. Ca2+ ...

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

在体内果蝇前运动神经末端的单分子跟踪

An increasing number of super-resolution microscopy techniques are helping to uncover the mechanisms that govern the nanoscale cellular world. ...

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

新生小鼠脑巴细胞基因调控的立体定向手术

Many genes are expressed in embryonic brains, and some of them are continuously expressed in the brain after birth. For such persistently expressed genes, ...