Name: presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry
Uploaded: 2012-01-12T13:00:00
Description: Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Finansiering fra National Institute for alkoholmisbrug og alkoholisme (NIAAA; AA-016967 og AA016967-01S1, TAM), start Wayne State University op fonde, og Wayne State University Research Grant Program. Indholdet er alene forfatternes ansvar og repræsenterer ikke det officielle synspunkter NIAAA eller National Institutes of Health.

1. Eksperimentel Essentials Elektrode Fabrication <ul type="circle"><li> Der er mange carbon fiber mikroelektroder fabrikation metoder, da de fleste er lavet in-house. Typisk hvad dikterer elektroden fabrikation detaljerne er den elektrokemiske teknik, der anvendes til elektroden (f.eks amperometry vs FSCV). For FSCV, kan mikroelektroder ske i huset ved hjælp af følgende tre-trins procedure. For en mere komplet beskrivelse af kulfiber elektrode fabrikation, se en nylig JOVE artikel 1.<...

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Den protokol, der præsenteres her viser hvordan man udarbejder og bruge musen koronale hjernen skiver for FSCV eksperimenter. Selv om denne metode er specifik for at opnå og måle dopamin dynamik, andre neurotransmittere som adenosin, er hydrogenperoxid, noradrenalin og serotonin blevet overvåget i vivo eller in vitro med FSCV 3, 8 - 11. FSCV kan bruges til at overvåge nogle af disse andre neurochemicals ved simple ændringer i bølgeform anvendes på arbejde elektrode 3, 11. ...

<table border="1"><tbody><tr><td> Navn </td><td> Firma </td><td> CAS-nummer </td></tr><tr><td width="274"> Reagens </td><td></td><td></td></tr><tr><td width="274"> Kaliumklorid </td><td> Fisher </td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274"> Natriumchlorid </td><td> EMD Kemikalier </td><td width="142" valign="top"> 7647145 </td></tr><tr><td width="274"> Magnesiumchlorid </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274"> Calciumchlorid </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274"> Natriumbicarbonat </td><td width="194" valign="top"> EMD Kemikalier </td><td width="142" valign="top"> 144558 </td></tr><tr><td width="274"> Natrium, dibasisk </td><td width="194" valign="top"> EMD Kemisks </td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D-glucose </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 50997 </td></tr><tr><td width="274"> Ascorbinsyre </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 50817 </td></tr><tr><td width="274"> Saccharose </td><td width="194" valign="top"> Fisher </td><td> 57501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> Materialer </td><td width="194" valign="top"> Vendor </td><td> Katalog nummer </td></tr><tr><td width="274"> Carbon fiber </td><td> Goodfellow Oakdale, PA </td><td></td></tr><tr><td width="274"> Glas kapillarrør </td><td> AM Systems Carlsborg WA. </td><td> 602000 </td></tr><tr><td width="274"> Silver wire </td><td> A-M Systems Carlsborg WA. </td><td> 787000 </td></tr><tr><td width="274"> Wolfram stimulerende elektrode </td><td> Plast One, Roanoke, VA </td><td></td></tr><tr><td width="274"> Platintraad </td><td></td><td></td></tr><tr><td width="274"> Ledning </td><td> Squires Elektronik, Cornelius, OR </td><td></td></tr><tr><td width="274"> Loctite 404 Hurtiglim </td><td> Hankal Corp Rocky Hill CT. </td><td></td></tr><tr><td width="274"> Barberblad </td><td> Verdens præcision Instruments Inc. FL. </td><td></td></tr><tr><td width="274"> BD Spinal nål </td><td> BD Medical Systems, Franlin Lake, NJ </td><td> REF 405234 </td></tr><tr><td width="274"> Kirurgisk Blade </td><td> Feather Safety barberkniv Co LTD. Japan </td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> Software </td><td width="194" valign = &quot;top&quot;&gt; Vendor </td><td> Katalog nummer </td></tr><tr><td width="274"> TH-software </td><td> ESA Inc., Chelmsford, MA </td><td></td></tr><tr><td width="274"> Instrument </td><td width="194" valign="top"> Vendor </td><td> Katalog nummer </td></tr><tr><td width="274"> Nedsænkning optagelse kammer </td><td> Brugerdefineret Videnskabelige, Denver, CO </td><td></td></tr><tr><td width="274"> Neorolog stimulus isolator </td><td> Digitmeter, Hertfordshire, England </td><td></td></tr><tr><td width="274"> Automatisk temperaturregulator </td><td> Warner Instrument Corporation </td><td></td></tr><tr><td width="274"> Mikroskop (SZX7) </td><td> Olympus </td><td></td></tr><tr><td width="274"> Microscope </td><td> Fisher </td><td></td></tr><tr><td width="274"> Vibratome 3000 sektionering systemet </td><td> St. Louer, MO. </td><td></td></tr><tr><td width="274"> Perfusion </td><td> Watson Marlow Limited, Falmouth Cornwall - England </td><td> H110708 </td></tr><tr><td width="274"> Mikropipette aftrækker </td><td> Narishige, Tokyo, Japan </td><td></td></tr><tr><td width="274"> ChemClamp potentiostat </td><td> Dagan Corporations, Minneapolis, MN. </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

Omfattende forskning har fokuseret på neurotransmitteren dopamin grund af dens betydning i virkningsmekanismen af ​​misbrugsstoffer (fx kokain og amfetamin), den rolle, den spiller i psykiatriske lidelser (fx skizofreni og Attention Deficit Hyperactivity Disorder), og dets engagement i degenerative lidelser som Parkinsons og Huntingtons sygdom. Under normale fysiologiske forhold, er dopamin kendt for at regulere motorisk aktivitet, kognition, læring, følelsesmæssig affekt og neuroendokrine hormon sekretion. En af de største tætheder af dopamin neuroner i striatum, som kan opdeles i to adskilte neuroanatomiske regioner kaldet nucleus accumbens og caudatus, putamen. Formålet er at illustrere en generel protokol for skive hurtigt scan cyklisk voltammetri (FSCV) inden musen striatum. FSCV er et veldefineret elektrokemisk teknik giver mulighed for at måle dopamin frigivelse og optagelse i realtid i discrete hjernen regioner. Kulfiber mikroelektroder (diameter ~ 7 μm) anvendes i FSCV at opdage dopamin oxidation. Den analytiske fordel ved at bruge FSCV at opdage dopamin er dens forbedret tidsmæssige opløsning på 100 millisekunder og rumlig opløsning på mindre end ti mikrometer, der giver supplerende oplysninger til in vivo mikrodialyse.

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Brug af Fast-scan cyklisk voltammetri at måle elektrisk fremkaldte præsynaptiske dopamin dynamik i striatale hjernen skiver.

Præsynaptiske dopamin Dynamics i striatale Brain Skiver med Fast-scan Cycliske voltammetri

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

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Neuroscience

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