Name: presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry
Uploaded: 2012-01-12T13:00:00
Description: Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Financiering door het National Institute on Alcohol Abuse en Alcoholism (NIAAA, AA-016967 en AA016967-01S1, TAM), Wayne State University opstarten fondsen, en Wayne State University Research Grant Program. De inhoud is uitsluitend de verantwoordelijkheid van de auteurs en vertegenwoordigt niet de officiële standpunten van NIAAA of de National Institutes of Health.

1. Experimentele essentials Electrode Fabrication <ul type="circle"><li> Er zijn tal van koolstofvezel micro-elektroden fabricagemethoden omdat de meeste zijn gemaakt in eigen huis. Typisch wat dicteert de elektrode fabricage details is de elektrochemische techniek die wordt toegepast op de elektrode (bijv. amperometrie versus FSCV). Voor FSCV, kunnen micro-elektroden worden in eigen huis gemaakt met behulp van de volgende drie-stappen procedure. Voor een meer volledige beschrijving van carbo...

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Het protocol hier gepresenteerde laat zien hoe voor te bereiden en de muis coronale de hersenen plakjes te gebruiken voor FSCV experimenten. Hoewel deze methode is specifiek voor het verkrijgen en het meten van dopamine dynamiek, andere neurotransmitters zoals adenosine, hebben waterstofperoxide, noradrenaline en serotonine zijn gemonitord in vivo of in vitro met FSCV 3, 8 - 11. FSCV kan worden gebruikt om sommige van deze andere neurotransmitters monitor door eenvoudige aanpassingen van de ...

<table border="1"><tbody><tr><td> Naam </td><td> Vennootschap </td><td> CAS-nummer </td></tr><tr><td width="274"> Reagens </td><td></td><td></td></tr><tr><td width="274"> Kalium Chloride </td><td> Visser </td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274"> Natriumchloride </td><td> Merck Chemicals </td><td width="142" valign="top"> 7647145 </td></tr><tr><td width="274"> Magnesiumchloride </td><td width="194" valign="top"> Visser </td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274"> Calciumchloride </td><td width="194" valign="top"> Visser </td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274"> Natriumbicarbonaat </td><td width="194" valign="top"> Merck Chemicals </td><td width="142" valign="top"> 144558 </td></tr><tr><td width="274"> Natriumfosfaat, Tweebasisch </td><td width="194" valign="top"> EMD Chemisches </td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D-glucose </td><td width="194" valign="top"> Visser </td><td width="142" valign="top"> 50997 </td></tr><tr><td width="274"> Ascorbinezuur </td><td width="194" valign="top"> Visser </td><td width="142" valign="top"> 50817 </td></tr><tr><td width="274"> Sucrose </td><td width="194" valign="top"> Visser </td><td> 57501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> Materialen </td><td width="194" valign="top"> Verkoper </td><td> Catalogus nummer </td></tr><tr><td width="274"> Carbon fiber </td><td> Goodfellow Oakdale, PA </td><td></td></tr><tr><td width="274"> Glazen capillaire </td><td> AM Systems Carlsborg WA. </td><td> 602000 </td></tr><tr><td width="274"> Zilverdraad </td><td> A-M Systems Carlsborg WA. </td><td> 787000 </td></tr><tr><td width="274"> Wolfraam elektrode </td><td> Plastics One, Roanoke, VA </td><td></td></tr><tr><td width="274"> Platina draad </td><td></td><td></td></tr><tr><td width="274"> Lead draad </td><td> Squires Electronics, Cornelius, OR </td><td></td></tr><tr><td width="274"> Loctite 404 Snellijm </td><td> Hankal Corp Rocky Hill CT. </td><td></td></tr><tr><td width="274"> Scheermesje </td><td> Wereld precisie-instrumenten Inc FL. </td><td></td></tr><tr><td width="274"> BD Spinal naald </td><td> BD Medical systemen, Franlin Lake, NJ </td><td> REF 405234 </td></tr><tr><td width="274"> Chirurgische Blade </td><td> Feather scheermes Co LTD. Japan </td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> Software </td><td width="194" valign = &quot;top&quot;&gt; Verkoper </td><td> Catalogus nummer </td></tr><tr><td width="274"> TH-software </td><td> ESA Inc, Chelmsford, MA </td><td></td></tr><tr><td width="274"> Instrument </td><td width="194" valign="top"> Verkoper </td><td> Catalogus nummer </td></tr><tr><td width="274"> Onderdompeling opname kamer </td><td> Custom Scientific, Denver, CO </td><td></td></tr><tr><td width="274"> Neorolog stimulus isolator </td><td> Digitmeter, Hertfordshire, Engeland </td><td></td></tr><tr><td width="274"> Automatische temperatuurregelaar </td><td> Warner Instrument Corporation </td><td></td></tr><tr><td width="274"> Microscoop (SZX7) </td><td> Olympus </td><td></td></tr><tr><td width="274"> Microscoop </td><td> Visser </td><td></td></tr><tr><td width="274"> Vibratome 3000 snijden systeem </td><td> St. Louis, MO. </td><td></td></tr><tr><td width="274"> Perfusie pomp </td><td> Watson Marlow Limited, Falmouth Cornwall - Engeland </td><td> H110708 </td></tr><tr><td width="274"> Micropipet puller </td><td> Narishige, Tokyo, Japan </td><td></td></tr><tr><td width="274"> ChemClamp potentiostaat </td><td> Dagan Bedrijven, Minneapolis, MN. </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

Uitgebreid onderzoek heeft zich gericht op de neurotransmitter dopamine, omdat het belang ervan in het werkingsmechanisme van drugs (bv cocaïne en amfetamine), de rol die het speelt in psychiatrische ziekten (zoals schizofrenie en Attention Deficit Hyperactivity Disorder), en haar betrokkenheid bij degeneratieve aandoeningen zoals Parkinson en de ziekte van Huntington. Onder normale fysiologische omstandigheden, is dopamine bekend locomotorische activiteit, cognitie, leren, emotionele invloed op, en neuro-endocriene hormoon secretie reguleren. Een van de grootste dichtheid van dopamine neuronen is in het striatum, die kan worden opgedeeld in twee verschillende neuroanatomische regio bekend als de nucleus accumbens en de caudate-putamen. Het doel is om een ​​algemeen protocol voor slice fast-scan cyclische voltammetrie (FSCV) illustreren binnen de muis striatum. FSCV is een goed gedefinieerd elektrochemische techniek biedt de mogelijkheid om dopamine afgifte en opname te meten in real-time in discrete hersengebieden. Carbon fiber micro-elektroden (diameter van ~ 7 um) worden gebruikt in FSCV om dopamine oxidatie te detecteren. De analytische voordeel van het gebruik FSCV aan dopamine te detecteren is de verbeterde temporele resolutie van 100 milliseconden en ruimtelijke resolutie van minder dan tien micrometer, die aanvullende gegevens in vivo microdialyse.

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Met behulp van fast-scan cyclische voltammetrie elektrisch opgeroepen presynaptische dopamine dynamiek te meten in de hersenen striatale plakjes.

Presynaptische Dopamine Dynamiek in het striatum Brain Plakjes met Fast-scan cyclische voltammetrie

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

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