Name: presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry
Uploaded: 2012-01-12T13:00:00
Description: Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Le financement fourni par le National Institute on Alcohol Abuse and Alcoholism (NIAAA, AA-016 967 et AA016967-01S1; TAM), Wayne State University fonds de démarrage, et la Wayne State University Research Grant Programme. Le contenu est exclusivement la responsabilité de leurs auteurs et ne représentent pas les vues officielles de NIAAA ou les National Institutes of Health.

1. L&#39;essentiel expérimentale Fabrication des électrodes <ul type="circle"><li> Il existe de nombreuses méthodes de fabrication de fibre de carbone microélectrodes car la plupart sont fabriqués en interne. Typiquement ce que dicte les détails de fabrication de l&#39;électrode est la technique électrochimique qui est appliqué à l&#39;électrode (par exemple, ampérométrie vs FSCV). Pour FSCV, microélectrodes peuvent être faites en interne en utilisant les trois étapes suivant ...

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Le protocole présenté ici montre comment préparer et utiliser des tranches de cerveau de souris pour des expériences FSCV coronale. Bien que cette méthode est spécifique à l&#39;obtention et la mesure de la dynamique de la dopamine, d&#39;autres neurotransmetteurs tels que l&#39;adénosine, le peroxyde d&#39;hydrogène, la noradrénaline et la sérotonine ont été suivis in vivo ou in vitro avec FSCV 3, 8 - 11. FSCV peut être utilisé pour surveiller certains de ces autres neurotran...

<table border="1"><tbody><tr><td> Nom </td><td> Société </td><td> Numéro CAS </td></tr><tr><td width="274"> Réactif </td><td></td><td></td></tr><tr><td width="274"> Chlorure de Potassium </td><td> Fisher </td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274"> Le chlorure de sodium </td><td> EMD Chemicals </td><td width="142" valign="top"> 7647145 </td></tr><tr><td width="274"> Le chlorure de magnésium </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274"> Le chlorure de calcium </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274"> Le bicarbonate de sodium </td><td width="194" valign="top"> EMD Chemicals </td><td width="142" valign="top"> 144558 </td></tr><tr><td width="274"> Phosphate de sodium dibasique </td><td width="194" valign="top"> EMD chimiques </td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D-glucose </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 50997 </td></tr><tr><td width="274"> L&#39;acide ascorbique </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 50817 </td></tr><tr><td width="274"> Saccharose </td><td width="194" valign="top"> Fisher </td><td> 57501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> Matériaux </td><td width="194" valign="top"> Vendeur </td><td> Numéro de catalogue </td></tr><tr><td width="274"> La fibre de carbone </td><td> Goodfellow Oakdale, PA </td><td></td></tr><tr><td width="274"> Capillaires en verre </td><td> AM Systems Carlsborg WA. </td><td> 602000 </td></tr><tr><td width="274"> Fil d&#39;Argent </td><td> A-M Systems Carlsborg WA. </td><td> 787000 </td></tr><tr><td width="274"> Électrode de tungstène stimulante </td><td> Plastics One, Roanoke, Virginie </td><td></td></tr><tr><td width="274"> Fil de platine </td><td></td><td></td></tr><tr><td width="274"> Le plomb métallique </td><td> Electronics Squires, Corneille, OU </td><td></td></tr><tr><td width="274"> Adhésif Loctite 404 instantanée </td><td> Hankal Corp Rocky Hill CT. </td><td></td></tr><tr><td width="274"> Lame de rasoir </td><td> Mondiale de précision Instruments Inc Floride. </td><td></td></tr><tr><td width="274"> BD épinière aiguille </td><td> BD Medical Systems, Franlin Lake, NJ </td><td> REF 405234 </td></tr><tr><td width="274"> Lame chirurgicale </td><td> Plume de rasoirs de sûreté Co. LTD. Le Japon </td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> Logiciel </td><td width="194" valign = &quot;top&quot;&gt; Vendeur </td><td> Numéro de catalogue </td></tr><tr><td width="274"> Logiciel de TH </td><td> ESA Inc, Chelmsford, MA </td><td></td></tr><tr><td width="274"> Instrument </td><td width="194" valign="top"> Vendeur </td><td> Numéro de catalogue </td></tr><tr><td width="274"> Chambre d&#39;enregistrement de submersion </td><td> Personnalisé scientifique, Denver, CO </td><td></td></tr><tr><td width="274"> Sectionneur de relance Neorolog </td><td> Digitmeter, Hertfordshire, Angleterre </td><td></td></tr><tr><td width="274"> Régulateur de température automatique </td><td> Warner Instrument Corporation </td><td></td></tr><tr><td width="274"> Microscope (SZX7) </td><td> Olympus </td><td></td></tr><tr><td width="274"> Microscope </td><td> Fisher </td><td></td></tr><tr><td width="274"> Système de sectionnement Vibratome 3000 </td><td> Saint-Louest, MO. </td><td></td></tr><tr><td width="274"> Pompe à perfusion </td><td> Watson Marlow Limited, Falmouth Cornouailles - Angleterre </td><td> H110708 </td></tr><tr><td width="274"> Micropipette extracteur </td><td> Narishige, Tokyo, Japon </td><td></td></tr><tr><td width="274"> ChemClamp potentiostat </td><td> Sociétés Dagan, Minneapolis, MN. </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

Des recherches approfondies ont porté sur la dopamine, un neurotransmetteur en raison de son importance dans le mécanisme d&#39;action de l&#39;abus des drogues (cocaïne et d&#39;amphétamines par exemple), le rôle qu&#39;elle joue dans les maladies psychiatriques (schizophrénie et de trouble d&#39;hyperactivité avec déficit de l&#39;attention), et son implication dans dégénératives troubles comme le Parkinson et la maladie de Huntington. Sous des conditions physiologiques normales, la dopamine est connue pour réguler l&#39;activité locomotrice, la cognition, l&#39;apprentissage, affectent émotionnel, et la sécrétion d&#39;hormones neuroendocrines. Une des plus grandes densités de neurones dopaminergiques est dans le striatum, qui peut être divisé en deux régions distinctes neuroanatomiques connu comme le noyau accumbens et le noyau caudé-putamen. L&#39;objectif est d&#39;illustrer un protocole général pour la voltamétrie cyclique tranche de balayage rapide (FSCV) dans le striatum de souris. FSCV est une technique bien définie électrochimique fournissant l&#39;occasion de mesurer la libération de dopamine et de l&#39;absorption en temps réel dans drégions du cerveau iscrete. Microélectrodes en fibre de carbone (diamètre de ~ 7 pm) sont utilisés dans FSCV pour détecter l&#39;oxydation de dopamine. L&#39;avantage d&#39;utiliser des analyses pour détecter FSCV dopamine est sa résolution temporelle accrue de 100 millisecondes et une résolution spatiale de moins de dix microns, fournissant des informations complémentaires à la microdialyse in vivo.

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

L&#39;utilisation de voltampérométrie cyclique balayage rapide pour mesurer la dynamique évoquée électriquement présynaptique de la dopamine dans le striatum des tranches de cerveau.

Dynamique présynaptique de la dopamine dans le striatum avec des tranches de cerveau de voltampérométrie cyclique à balayage rapide

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

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