Name: presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry
Uploaded: 2012-01-12T13:00:00
Description: Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Die Förderung des National Institute on Alcohol Abuse and Alcoholism (NIAAA; AA-016967 und AA016967-01S1; TAM), Start der Wayne State University von Fonds und der Wayne State University Research Grant Program. Der Inhalt ist ausschließlich in der Verantwortung der Autoren und repräsentieren nicht die offiziellen Ansichten der NIAAA oder den National Institutes of Health.

1. Experimentelle essentials Electrode Fabrication <ul type="circle"><li> Es gibt zahlreiche Kohlefaser-Mikroelektroden Herstellungsmethoden, da die meisten in-house gemacht werden. In der Regel, was bestimmt die Elektrode Herstellung Details ist das elektrochemische Verfahren, das an der Elektrode (zB Amperometrie vs FSCV) angewendet wird. Für FSCV können Mikroelektroden in-house durchgeführt werden mit dem folgenden Drei-Stufen-Verfahren. Für eine vollständige Beschreibung der Kohlefas...

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Das Protokoll hier vorgestellten demonstriert, wie die Vorbereitung und Verwendung der Maus koronalen Hirnschnitten für FSCV Experimente. 11 - Diese Methode ist zwar spezifisch für den Erhalt und die Messung Dopamin Dynamik, andere Neurotransmitter wie Adenosin, haben Wasserstoffperoxid, Noradrenalin und Serotonin in vivo oder in vitro mit FSCV 3, 8 überwacht. FSCV können verwendet werden, um einige dieser anderen neurochemicals durch einfache Modifikationen der Wellenform ang...

<table border="1"><tbody><tr><td> Name </td><td> Firma </td><td> CAS-Nummer </td></tr><tr><td width="274"> Reagens </td><td></td><td></td></tr><tr><td width="274"> Kaliumchlorid </td><td> Fischer </td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274"> Kochsalz </td><td> EMD Chemicals </td><td width="142" valign="top"> 7647145 </td></tr><tr><td width="274"> Magnesiumchlorid </td><td width="194" valign="top"> Fischer </td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274"> Calciumchlorid </td><td width="194" valign="top"> Fischer </td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274"> Natriumbicarbonat </td><td width="194" valign="top"> EMD Chemicals </td><td width="142" valign="top"> 144558 </td></tr><tr><td width="274"> Natriumphosphat, Dibasic </td><td width="194" valign="top"> EMD Chemicals </td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D-Glucose </td><td width="194" valign="top"> Fischer </td><td width="142" valign="top"> 50997 </td></tr><tr><td width="274"> Askorbinsäure </td><td width="194" valign="top"> Fischer </td><td width="142" valign="top"> 50817 </td></tr><tr><td width="274"> Sucrose </td><td width="194" valign="top"> Fischer </td><td> 57501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> Materialien </td><td width="194" valign="top"> Verkäufer </td><td> Katalog-Nummer </td></tr><tr><td width="274"> Kohlefaser </td><td> Goodfellow Oakdale, PA </td><td></td></tr><tr><td width="274"> Glaskapillare </td><td> AM-Systeme Carlsborg WA. </td><td> 602000 </td></tr><tr><td width="274"> Silberdraht </td><td> A-M-Systeme Carlsborg WA. </td><td> 787000 </td></tr><tr><td width="274"> Tungsten Stimulationselektrode </td><td> Plastics One, Roanoke, VA </td><td></td></tr><tr><td width="274"> Platindraht </td><td></td><td></td></tr><tr><td width="274"> Anschlusskabel </td><td> Squires Electronics, Cornelius, OR </td><td></td></tr><tr><td width="274"> Loctite 404 Sekundenkleber </td><td> Hankal Corp Rocky Hill CT. </td><td></td></tr><tr><td width="274"> Rasierklinge </td><td> World Precision Instruments Inc. FL. </td><td></td></tr><tr><td width="274"> BD Spinalnadel </td><td> BD Medical Systems, Franlin Lake, NJ </td><td> REF 405234 </td></tr><tr><td width="274"> Chirurgische Klinge </td><td> Feather Rasierklingen Co. LTD. Japan </td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> Software </td><td width="194" valign = &quot;top&quot;&gt; Verkäufer </td><td> Katalog-Nummer </td></tr><tr><td width="274"> TH-Software </td><td> ESA Inc., Chelmsford, MA </td><td></td></tr><tr><td width="274"> Instrument </td><td width="194" valign="top"> Verkäufer </td><td> Katalog-Nummer </td></tr><tr><td width="274"> Eintauchen Aufnahme Kammer </td><td> Benutzerdefinierte Scientific, Denver, CO </td><td></td></tr><tr><td width="274"> Neorolog Stimulus Isolator </td><td> Digitmeter, Hertfordshire, England </td><td></td></tr><tr><td width="274"> Automatische Temperaturregelung </td><td> Warner Instrument Corporation </td><td></td></tr><tr><td width="274"> Microscope (SZX7) </td><td> Olymp </td><td></td></tr><tr><td width="274"> Mikroskop </td><td> Fischer </td><td></td></tr><tr><td width="274"> Vibratom 3000 Schneidesystem </td><td> St. Louist, MO. </td><td></td></tr><tr><td width="274"> Perfusionspumpe </td><td> Watson Marlow Limited, Falmouth Cornwall - England </td><td> H110708 </td></tr><tr><td width="274"> Micropipette puller </td><td> Narishige, Tokyo, Japan </td><td></td></tr><tr><td width="274"> ChemClamp Potentiostaten </td><td> Dagan Unternehmen, Minneapolis, MN. </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

Umfangreiche Forschung hat sich auf den Neurotransmitter Dopamin wegen ihrer Bedeutung in der Wirkmechanismus von Drogen (zB Kokain und Amphetamin), seine Rolle in psychiatrischen Erkrankungen (zB Schizophrenie und Aufmerksamkeits-Defizit-Syndrom mit Hyperaktivität), und seine Einbindung in degenerative konzentriert Erkrankungen wie Parkinson und Chorea Huntington. Unter normalen physiologischen Bedingungen wird Dopamin bekannt, Bewegungsaktivität, Kognition, Lernen, emotionale Auswirkungen, und neuroendokrinen Sekretion regulieren. Eines der größten Dichten von Dopamin-Neuronen innerhalb des Striatum, die in zwei unterschiedlichen neuroanatomischen Regionen wie der Nucleus accumbens und die caudate-Putamen bekannt aufgeteilt werden kann. Ziel ist es, ein allgemeines Protokoll für Stück Fast-Scan-Cyclovoltammetrie (FSCV) in der Maus Striatum zu illustrieren. FSCV ist eine gut definierte elektrochemische Technik bietet die Möglichkeit, die Freisetzung von Dopamin und Aufnahme zu messen und in Echtzeit in discrete Hirnregionen. Kohlefaser Mikroelektroden (Durchmesser von ca. 7 um) sind in FSCV zur Dopamin-Oxidation zu erkennen. Die analytische Vorteil der Verwendung FSCV zu Dopamin zu erkennen ist die erweiterte zeitliche Auflösung von 100 Millisekunden und räumlichen Auflösung von weniger als zehn Mikrometer, das ergänzende Informationen zur In-vivo-Mikrodialyse.

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Mit Fast-Scan-Cyclovoltammetrie zu elektrisch evozierten präsynaptischen Dopamin-Dynamik in striatalen Hirnschnitten zu messen.

Präsynaptische Dynamics in striatalen Hirnschnitten mit Fast-Scan-Cyclovoltammetrie

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

Research

JoVE Journal

Neuroscience

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals

Vibrodissociation von Neuronen aus Nager Hirnschnitten zu Study Synaptic Transmission and Image präsynaptischen

Mechanical dissociation of neurons from the central nervous system has the advantage that presynaptic boutons remain attached to the isolated neuron of ...

Forschung

Neurowissenschaften

Multi-electrode Array Recordings of Neuronal Avalanches in Organotypic Cultures

Multi-Elektroden-Array Recordings neuronaler Lawinen in organotypischen Kulturen

The cortex is spontaneously active, even in the absence of any particular input or motor output.  During development, this activity is important for the ...

Voltage Biasing, Cyclic Voltammetry, &amp; Electrical Impedance Spectroscopy for Neural Interfaces

Voltage Biasing, Cyclic Voltammetry, & Electrical Impedance Spectroscopy for Neural Interfaces

Vorspannschaltung, Cyclovoltammetrie, Impedanzspektroskopie &amp; Elektrotechnik für Neuronale Interfaces

Electrical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measure properties of the electrode-tissue interface without additional invasive ...

Paired Whole Cell Recordings in Organotypic Hippocampal Slices

Gepaart ganze Zelle Recordings in Organotypische Hippocampus

Pair recordings involve simultaneous whole cell patch clamp recordings from two synaptically connected neurons, enabling not only direct ...

Whole-cell Patch-clamp Recordings in Brain Slices

Whole-Cell-Patch-Clamp-Aufnahmen in Hirnschnitten

Whole-cell patch-clamp recording is an electrophysiological technique that allows the study of the&#160;electrical properties of a substantial part of the ...

Modeling Fast-scan Cyclic Voltammetry Data from Electrically Stimulated Dopamine Neurotransmission Data Using QNsim1.0

Modellierung Fast-Scan Zyklische Voltammetrie Daten aus elektrisch stimulierten Dopamin Neurotransmission Daten mit QNsim1.0

Central dopaminergic (DAergic) pathways have an important role in a wide range of functions, such as attention, motivation, and movement. Dopamine (DA) is ...

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices

Bewertung der Synapse Dichte im Hippokampus Nagetier Gehirnscheiben

In the brain, synapses are specialized junctions between neurons, determining the strength and spread of neuronal signaling. The number of synapses is ...

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Zwei-Photonen-Kalzium Imaging in neuronalen Dendriten in Hirnschnitten

Calcium (Ca2+) imaging is a powerful tool to investigate the spatiotemporal dynamics of intracellular Ca2+ signals in neuronal dendrites. Ca2+ ...

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

In Vivo Single-Molecule Tracking at the Drosophila Presynaptic Motor Nerve Terminal

In Vivo Einzelmolekül-Tracking bei Drosophila präsynaptischen motorischen Nerv Terminal

An increasing number of super-resolution microscopy techniques are helping to uncover the mechanisms that govern the nanoscale cellular world. ...

Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

Stereotaktischen Chirurgie für Genmanipulation in Striatale Zellen des Neugeborenen Mäusegehirnen

Many genes are expressed in embryonic brains, and some of them are continuously expressed in the brain after birth. For such persistently expressed genes, ...