Name: presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry
Uploaded: 2012-01-12T13:00:00
Description: Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Finansiering från National Institute on alkoholmissbruk och alkoholism (NIAAA, AA-016.967 och AA016967-01S1, TAM), Wayne State University starta fonder, och Wayne State University Research Grant Program. Innehållet är ensamt ansvarig för författare och utgör inte officiella ståndpunkter NIAAA eller National Institutes of Health.

1. Experimentell Essentials Elektrod Fabrication <ul type="circle"><li> Det finns många kolfiber microelectrodes tillverkningsmetoder eftersom de flesta är tillverkade i egen regi. Typiskt vad dikterar detaljerna elektroden tillverkning är elektrokemiska teknik som används till elektroden (t.ex. amperometry vs FSCV). För FSCV kan microelectrodes göras i egen regi med hjälp av följande tre steg. För en mer fullständig beskrivning av kolfiber elektrod bearbetning, se en nyligen JUPITE...

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Protokollet presenteras här visar hur man förbereder och använder musen koronala hjärnan skivor för FSCV experiment. Även om denna metod är specifik för att erhålla och mäta dopamin dynamik, adenosin andra signalsubstanser som har väteperoxid, noradrenalin och serotonin övervakats in vivo eller in vitro med FSCV 3, 8 - 11. FSCV kan användas för att övervaka några av dessa andra neurochemicals genom enkla ändringar av vågformen tillämpas på arbete elektroden 3, 11.</s...

<table border="1"><tbody><tr><td> Namn </td><td> Företag </td><td> CAS-nummer </td></tr><tr><td width="274"> Reagens </td><td></td><td></td></tr><tr><td width="274"> Kaliumklorid </td><td> Fisher </td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274"> Natriumklorid </td><td> EMD Kemikalier </td><td width="142" valign="top"> 7647145 </td></tr><tr><td width="274"> Magnesiumklorid </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274"> Kalciumklorid </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274"> Natriumbikarbonat </td><td width="194" valign="top"> EMD Kemikalier </td><td width="142" valign="top"> 144.558 </td></tr><tr><td width="274"> Natriumfosfat, dibasiskt </td><td width="194" valign="top"> EMD Kemisks </td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D-glukos </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 50.997 </td></tr><tr><td width="274"> Askorbinsyra </td><td width="194" valign="top"> Fisher </td><td width="142" valign="top"> 50.817 </td></tr><tr><td width="274"> Sackaros </td><td width="194" valign="top"> Fisher </td><td> 57.501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> Material </td><td width="194" valign="top"> Leverantör </td><td> Katalognummer </td></tr><tr><td width="274"> Kolfiber </td><td> Goodfellow Oakdale, PA </td><td></td></tr><tr><td width="274"> Glas kapillär </td><td> AM System Carlsborg WA. </td><td> 602 tusen </td></tr><tr><td width="274"> Silvertråd </td><td> A-M Systems Carlsborg WA. </td><td> 787 tusen </td></tr><tr><td width="274"> Tungsten stimulerande elektrod </td><td> Plast One, Roanoke, VA </td><td></td></tr><tr><td width="274"> Platinatråd </td><td></td><td></td></tr><tr><td width="274"> Blytråd </td><td> Squires Elektronik, Cornelius, OR </td><td></td></tr><tr><td width="274"> Loctite 404 Snabblim </td><td> Hankal Corp Rocky Hill CT. </td><td></td></tr><tr><td width="274"> RAKBLAD </td><td> Världen precision Instruments Inc. FL. </td><td></td></tr><tr><td width="274"> BD Spinal nål </td><td> BD Medical Systems, Franlin Lake, NJ </td><td> REF 405.234 </td></tr><tr><td width="274"> Kirurgiska Blade </td><td> Feather rakhyvlar Co LTD. Japan </td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> Programvara </td><td width="194" valign = &quot;top&quot;&gt; Leverantör </td><td> Katalognummer </td></tr><tr><td width="274"> TH-programvara </td><td> ESA Inc., Chelmsford, MA </td><td></td></tr><tr><td width="274"> Instrument </td><td width="194" valign="top"> Leverantör </td><td> Katalognummer </td></tr><tr><td width="274"> Nedsänkning inspelning kammare </td><td> Anpassad vetenskapliga, Denver, CO </td><td></td></tr><tr><td width="274"> Neorolog stimulans isolator </td><td> Digitmeter, Hertfordshire, England </td><td></td></tr><tr><td width="274"> Automatisk temperaturreglering </td><td> Warner Instrument Corporation </td><td></td></tr><tr><td width="274"> Mikroskop (SZX7) </td><td> Olympus </td><td></td></tr><tr><td width="274"> Mikroskop </td><td> Fisher </td><td></td></tr><tr><td width="274"> Vibratome 3000 sektionering systemet </td><td> St Louär, MO. </td><td></td></tr><tr><td width="274"> Perfusion pumpen </td><td> Watson Marlow Limited, Falmouth Cornwall - England </td><td> H110708 </td></tr><tr><td width="274"> Mikropipett avdragare </td><td> Narishige, Tokyo, Japan </td><td></td></tr><tr><td width="274"> ChemClamp potentiostat </td><td> Dagan Företag, Minneapolis, MN. </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

Omfattande forskning har fokuserat på signalsubstansen dopamin på grund av dess betydelse i verkningsmekanismen för missbruksmedel (t.ex. kokain och amfetamin), den roll den spelar i psykiska sjukdomar (t.ex. schizofreni och Attention Deficit Hyperactivity Disorder), och dess engagemang i degenerativa sjukdomar som Parkinsons och Huntingtons sjukdom. Under normala fysiologiska förhållanden är dopamin känt att reglera rörelseaktivitet, kognition, inlärning, emotionell påverkar och neuroendokrina hormon. En av de största tätheten av dopaminneuron är inom striatum, som kan indelas i två olika neuroanatomiska regionerna kallas kärnan accumbens och nucleus caudatus och putamen. Målet är att belysa ett generellt protokoll för bit snabb skanning cyklisk voltammetry (FSCV) inom musen striatum. FSCV är en väldefinierad elektrokemisk teknik som ger möjlighet att mäta dopamin utsläpp och upptag i realtid i discrete hjärnan. Kolfiber microelectrodes (diameter på ~ 7 mikrometer) används i FSCV att upptäcka dopamin oxidation. Den analytiska Fördelen med att använda FSCV att upptäcka dopamin är dess bättre tidsupplösning på 100 millisekunder och rumsliga upplösning på mindre än tio mikrometer, vilket ger kompletterande information in vivo mikrodialys.

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Använda snabb-scan cykliska voltammetry att mäta elektriskt framkallat presynaptiska dopamin dynamik i striatala hjärnan skivor.

Presynaptisk dopamin Dynamics i striatum hjärnan skivor med Fast-scan Cykliska Voltammetry

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

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