Name: presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry
Uploaded: 2012-01-12T13:00:00
Description: Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Ulusal Alkol İstismarı ve Alkolizm Enstitüsü (AA-016.967 ve AA016967-01S1; NIAAA TAM) tarafından sağlanan finansman, Wayne State Üniversitesi fonları başlatmak ve Wayne State Üniversitesi Araştırma Hibe Programı. Içeriği sadece yazarların sorumluluğundadır ve NIAAA veya Ulusal Sağlık Enstitüleri resmi görüşlerini temsil etmemektedir.

1. Deneysel şartları Elektrot Fabrikasyon <ul type="circle"><li> Çoğu in-house yapılır bu yana çok sayıda karbon fiber Mikroelektronlar imalat yöntemleri vardır. Tipik olarak elektrot imalat detayları dikte elektrot (örneğin amperometri vs FSCV) uygulanır elektrokimyasal bir tekniktir. Mikroelektronlar FSCV için, aşağıdaki üç adım yordamı kullanarak in-house olabilir. Karbon fiber elektrod fabrikasyon daha eksiksiz bir açıklaması için, yeni bir Jüpiter 1. madde...

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Burada sunulan protokol FSCV deneyler fare koronal beyin dilimleri hazırlamak ve nasıl kullanılacağını gösteriyor. 11 - Bu yöntem dopamin dinamiklerini elde etmek ve ölçmek için özel olmasına rağmen, diğer nörotransmitterlerin gibi adenozin, hidrojen peroksit, noradrenalin ve serotonin FSCV 3, 8 ile in vivo ve in vitro olarak takip edilmiştir. FSCV bazı çalışma elektrot 3, 11 uygulanan dalga basit değişiklikler ile bu diğer nörokimyasalların iz...

<table border="1"><tbody><tr><td> Isim </td><td> Şirket </td><td> CAS numarası </td></tr><tr><td width="274"> Reaktif </td><td></td><td></td></tr><tr><td width="274"> Potasyum klorid </td><td> Balıkçı </td><td width="142" valign="top"> 7447407 </td></tr><tr><td width="274"> Sodyum klorür </td><td> Merck Kimyasallar </td><td width="142" valign="top"> 7647145 </td></tr><tr><td width="274"> Magnezyum klorür </td><td width="194" valign="top"> Balıkçı </td><td width="142" valign="top"> 7791186 </td></tr><tr><td width="274"> Kalsiyum klorür </td><td width="194" valign="top"> Balıkçı </td><td width="142" valign="top"> 10035048 </td></tr><tr><td width="274"> Sodyum bikarbonat </td><td width="194" valign="top"> Merck Kimyasallar </td><td width="142" valign="top"> 144558 </td></tr><tr><td width="274"> Sodyum fosfat, Dibazik </td><td width="194" valign="top"> Merck Kimyasals </td><td width="142" valign="top"> 7558794 </td></tr><tr><td width="274"> D-glukoz </td><td width="194" valign="top"> Balıkçı </td><td width="142" valign="top"> 50997 </td></tr><tr><td width="274"> Askorbik asit </td><td width="194" valign="top"> Balıkçı </td><td width="142" valign="top"> 50817 </td></tr><tr><td width="274"> Sakaroz </td><td width="194" valign="top"> Balıkçı </td><td> 57501 </td></tr><tr><td width="274">&nbsp;</td><td width="194" valign="top">&nbsp;</td><td>&nbsp;</td></tr><tr><td width="274"> Malzemeler </td><td width="194" valign="top"> Satıcı </td><td> Katalog numarası </td></tr><tr><td width="274"> Karbon fiber </td><td> Goodfellow Oakdale, PA </td><td></td></tr><tr><td width="274"> Cam kapiller </td><td> AM Sistemleri Carlsborg WA. </td><td> 602000 </td></tr><tr><td width="274"> Gümüş tel </td><td> A-M Sistemleri Carlsborg WA. </td><td> 787000 </td></tr><tr><td width="274"> Tungsten uyarıcı elektrot </td><td> Plastik biri, Roanoke, VA </td><td></td></tr><tr><td width="274"> Platin tel </td><td></td><td></td></tr><tr><td width="274"> Kurşun tel </td><td> Squires Elektronik, Cornelius, OR </td><td></td></tr><tr><td width="274"> Loctite 404 anında yapıştırıcı </td><td> Hankal Corp Rocky Hill CT. </td><td></td></tr><tr><td width="274"> Jilet </td><td> Dünya hassas Instruments Inc FL. </td><td></td></tr><tr><td width="274"> BD Spinal iğne </td><td> BD Tıp sistemleri, Franlin Gölü, NJ </td><td> REF 405.234 </td></tr><tr><td width="274"> Cerrahi Bıçak </td><td> Tüy Güvenlik jilet LTD. Japonya </td><td></td></tr><tr><td width="274"></td><td></td><td></td></tr><tr><td width="274"> Yazılım </td><td width="194" valign = &quot;top&quot;&gt; Satıcı </td><td> Katalog numarası </td></tr><tr><td width="274"> TH yazılım </td><td> ESA A.Ş., Chelmsford, MA </td><td></td></tr><tr><td width="274"> Enstrüman </td><td width="194" valign="top"> Satıcı </td><td> Katalog numarası </td></tr><tr><td width="274"> Daldırma kayıt odası </td><td> Özel Scientific, Denver, CO </td><td></td></tr><tr><td width="274"> Neorolog uyaran izolatör </td><td> Digitmeter, Hertfordshire, İngiltere </td><td></td></tr><tr><td width="274"> Otomatik sıcaklık kontrolörü </td><td> Warner Instrument Corporation </td><td></td></tr><tr><td width="274"> Mikroskobu (SZX7) </td><td> Olympus </td><td></td></tr><tr><td width="274"> Mikroskop </td><td> Balıkçı </td><td></td></tr><tr><td width="274"> Vibratome 3000 kesit sistemi </td><td> St. Lou, MO. </td><td></td></tr><tr><td width="274"> Perfüzyon pompası </td><td> Watson Marlow Limited, Falmouth Cornwall - İngiltere </td><td> H110708 </td></tr><tr><td width="274"> Mikropipet çektirmesi </td><td> Narishige, Tokyo, Japonya </td><td></td></tr><tr><td width="274"> ChemClamp potansiyostat </td><td> Dagan Şirketler, Minneapolis, MN. </td><td></td></tr></tbody></table>

presynaptic dopamine dynamics in striatal brain slices with fast-scan cyclic voltammetry

Kapsamlı araştırma kötüye ilaçlar (örneğin kokain ve amfetamin), oynadığı rol psikiyatrik hastalıklar (örneğin şizofreni ve Dikkat Eksikliği Hiperaktivite Bozukluğu) ve dejeneratif katılımının eylem mekanizması önemi nedeniyle nörotransmiter dopamin odaklandı Parkinson ve Huntington hastalığı gibi bozukluklar. Normal fizyolojik koşullar altında, dopamin lokomotor aktivite, biliş, öğrenme, duygusal etkiler, ve nöroendokrin hormonu salgılamasını düzenleyen bilinmektedir. Dopamin nöronları en büyük yoğunluklarının biri, striatum, nukleus accumbens ve kaudat-putamen olarak bilinen iki ayrı nöroanatomik bölgelerdeki ayrılabilir içinde. Amaç fare striatum içinde dilim hızlı tarama döngüsel voltametri (FSCV) için genel bir protokol göstermek için. FSCV d gerçek zamanlı olarak, dopamin salınımı ve alımı ölçmek için fırsat sağlayan iyi tanımlanmış bir elektrokimyasal tekniğiiscrete beyin bölgeleri. FSCV Karbon fiber Mikroelektronlar dopamin oksidasyon (~ 7 mm çapında) tespit etmek için kullanılır. Dopamin algılamak için FSCV kullanarak analitik avantajı in vivo mikrodiyaliz tamamlayıcı bilgiler sağlayarak, en az on mikron 100 milisaniye ve uzaysal çözünürlüğü, gelişmiş temporal çözünürlük .

Watch this Scientific Journal Video about Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry at JoVE.com

Presynaptic Dopamine Dynamics in Striatal Brain Slices with Fast-scan Cyclic Voltammetry

Striatal beyin dilimleri elektriksel olarak uyarılmış presinaptik dopamin dinamiklerini ölçmek için hızlı tarama döngüsel voltametri kullanma.

Hızlı tarama Cyclic Voltametri striatal Beyin Slices presinaptik dopamin Dinamiği

Extensive research has focused on the neurotransmitter dopamine because of its importance in the mechanism of action of drugs of abuse (e.g. cocaine and ...

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Neuroscience

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