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DOI :
10.3791/3491-v
•
12:19 min
January 21st, 2012
Chapters
0:05
Title
1:19
Labeling and Fixing of Cells
2:30
Denaturing and Staining of Brdu and DNA
4:11
Flow Cytometry and Data Analysis
6:10
Alternate Protocol for Cell Cycle Analysis in a Mixed Population of Cells
7:35
Cell Cycle Distribution Staining Can Be Used for a Variety of Experimental Purposes
11:47
Conclusion
細胞集団の細胞周期の位置を決定する、または信号が増殖にどのような影響を与えるか理解する、容易にこのプロトコルを使用してフローサイトメトリーによって測定することができる。我々は、細胞を染色し、細胞周期内の位置を定量化するための簡単な実験的なアプローチを報告する。
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