Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/3495-v
•
6:09 min
January 18th, 2012
Chapters
0:05
Title
1:18
Preparing the Kinase Reactions and Running the Assay
3:30
Transferring the Samples and Analyzing Results
4:45
Results: Kinase Assay
5:49
Conclusion
富亮氨酸重复激酶2是一个大型的多域激酶,基因突变,其中最常见的帕金森氏病的遗传原因。这种蛋白激酶活性分析已被证明是在了解这种蛋白质的生物学和功能障碍的重要工具。在本文中,在体外化验的LRRK2激酶活性和描述及其突变体的选择,提供了一个实验系统研究假定基板和潜在的功能障碍疾病的LRRK2磷酸化。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved