Name: a chitosan based, laser activated thin film surgical adhesive, 'surgilux': preparation and demonstration
Uploaded: 2012-10-23T14:00:00
Description: Watch this Scientific Journal Video about A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration at JoVE.com

作者承认国家健康与医学研究委员会澳大利亚（NHMRC＃1000674）的资助LJR福斯特。

1。制备SurgiLux解决方案<ol><li>准备2％（体积/体积）溶液中，在一个干净的玻璃烧杯中，使用去离子水的乙酸，使用在层流罩，以避免污染。 </li><li>称取0.02％（重量/体积）的发色团，吲哚花青绿，ICG，在无菌的Eppendorf管中，确保管被包裹在银箔，以防止任何光线穿透。 </li><li>使用干净的，一次性吸液管，约1毫升的稀乙酸溶液转移到试管中，以溶解染料，轻轻摇动，并保持在箔包裹。 </li><li>转...

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	<li>Kjaergard, H. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suture+support:+is+it+advantageous.">Suture support: is it advantageous.</a> Am. J. Surg. 182, 15S-20S (2001).</li><li>Lauto, A., Mawad, D., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adhesive+biomaterials+for+tissue+reconstruction.">Adhesive biomaterials for tissue reconstruction.</a> J. Chem. Tech. Biotech. 83, 464-472 (2008).</li><li>Fung, L. C., Mingin, G. C., Massicotte, M., Felsen, D., Poppas, D. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+temperature+on+tissue+thermal+injury+and+wound+strength+after+photochemical+wound+closure.">Effects of temperature on tissue thermal injury and wound strength after photochemical wound closure.</a> Lasers Surg. Med. 25, 285-290 (1999).</li><li>Piribo, <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Glues &amp; Sealants: Industry Background Report. , (2005).</li><li>Lauto, A., Hook, J., Doran, M., Camacho, F., Poole-Warren, L. A., Avolio, A., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chitosan+adhesive+for+laser+tissue-welding:+in+vitro+characterisation.">Chitosan adhesive for laser tissue-welding: in vitro characterisation.</a> Lasers Surg. Med. 36, 193-201 (2005).</li><li>Lauto, A., Stoodley, M., Marcel, H., Avolio, A., Sarris, M., McKenzie, G., Sampson, D. D., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+and+in+vivo+tissue+repair+with+laser-activated+chitosan+adhesive.">In vitro and in vivo tissue repair with laser-activated chitosan adhesive.</a> Lasers Surg. Med. 39, 19-27 (2007).</li><li>Lauto, A., Foster, L. J. R., Avolio, A., Sampson, D., Raston, C., Sarris, M., McKenzie, G., Stoodley, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sutureless+Nerve+Repair+with+Laser-Activated+Chitosan+Adhesive:+A+Pilot+in+vivo+Study.">Sutureless Nerve Repair with Laser-Activated Chitosan Adhesive: A Pilot in vivo Study.</a> J. Photomed. Laser. Surg. 26 (3), 227-234 (2008).</li><li>Mar&#231;al, H., Badylak, S. F., Sellaro, T. L., Lauto, A., Foster, L. J. R., Mahler, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+coalescence+of+decellularized+tissue+scaffolds,+laser-activated+chitosan+bioadhesive+and+olfactory+ensheathing+cells+for+tissue+repair+and+regeneration+of+the+spinal+cord.">The coalescence of decellularized tissue scaffolds, laser-activated chitosan bioadhesive and olfactory ensheathing cells for tissue repair and regeneration of the spinal cord.</a> Lasers Med. Sci. 23 (1), 96 (2008).</li><li>Foster, L. J. R., Thomson, K., Marcal, H., Butt, J., Watson, S., Wakefield, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+chitosan-vancomycin+composite+biomaterial+as+a+laser+activated+surgical+adhesive+with+regional+antimicrobial+activity.">A chitosan-vancomycin composite biomaterial as a laser activated surgical adhesive with regional antimicrobial activity.</a> Biomacromolecules. 11 (12), 3563-3570 (2010).</li><li>Shahbazi, J., Marcal, H., Watson, S., Wakefield, D., Sarris, M., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sutureless+sealing+of+penetrating+corneal+wounds+using+a+laser-activated+thin+film+adhesive.">Sutureless sealing of penetrating corneal wounds using a laser-activated thin film adhesive.</a> Lasers Surg. Med. , .</li><li>Meyers, M. A., Chen, P. -. Y., Lin, A. Y. -. M., Seki, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biological+materials:+Structure+and+mechanical+properties.">Biological materials: Structure and mechanical properties.</a> Prog. Mater. Sci. 53 (1), 1-206 (2008).</li></ol>

脱乙酰壳多糖可以在多种不同的分子量和不同程度的去乙醯化具有协同性作用（DDA）获得。脱乙酰壳多糖的纯度的变化可能会导致存在的微粒在SurgiLux溶液;离心是用来消除这些和应导致在一个透明的绿色溶液。但是，过滤还可以被用作一个附加的或替代的制造工序。至于与任何材料的加工，变化，，如壳聚糖DDA和分子量，有所得SurgiLux薄膜，生物和物质的理化性质，包括其粘合到组织的强度的影?...

<table border="1"><tbody><tr><td> 的试剂/设备名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论（可选） </td></tr><tr><td>壳聚糖</td><td> Sigma-Aldrich公司</td><td> 448877 </td><td></td></tr><tr><td>吲哚菁绿</td><td> Sigma-Aldrich公司</td><td> I2633 </td><td>也被称为Cardiogreen </td></tr><tr><td>醋酸</td><td> Sigma-Aldrich公司</td><td> 320099 </td><td></td></tr><tr><td>红外二极管激光器与光纤传输。 （808纳米，120毫瓦，梁芯200微米） </td><td> CNI激光器</td><td> FC-808 </td><td>可变高达5 W电源系统</td></tr><tr><td>激光防护眼镜</td><td> CNI激光器</td><td> LS-G </td><td></td></tr><tr><td>拉伸试验设备</td><td>英斯特朗私人有限公司</td><td> 5542 </td><td> 50 N称重传感器</td></tr></tbody></table>

a chitosan based, laser activated thin film surgical adhesive, 'surgilux': preparation and demonstration

缝合线是一个4000年的老技术，保持伤口愈合的“金标准”凭借其的修复强度（〜100千帕）。然而，缝合线可以作为感染的一个核心，在许多程序无法影响伤口修复，或功能性组织再生的干扰。1手术胶水和粘合剂，如那些基于纤维蛋白和氰基丙烯酸酯，已经研制出用作替代缝线这种伤口修复。然而，目前的商业粘合剂也有显着的缺点，从病毒和朊病毒的转让和与血纤维蛋白胶水的维修强度缺乏，组织的毒性和缺乏的生物相容性的氰基丙烯酸酯为基础的粘合剂。此外，目前可用的手术粘合剂往往是凝胶为基础的，并且可以延长固化时间，这限制了它们的应用程序2同样地，使用的UV激光器，以促进交联机制在蛋白质为基础的或白蛋白&#39;溶胶德尔斯的可导致DNA损伤，而激光组织焊接（LTW）易患热损伤的组织。3尽管他们的缺点，粘合剂和LTW已捕获的伤口闭合市场约30％报道超过美元5星十亿每年，一个重要的证明需要缝合的技术。 在无缝线技术的追求，我们利用壳聚糖作为一种生物材料发展的一个灵活的，薄膜，激光激活的手术粘合剂称为“SurgiLux”。这种新颖的的生物黏附使用FDA批准并成功应用于医学领域的应用和产品的各种生物材料和光子学相结合的独特。 SurgiLux克服与缝线和电流的手术粘合剂（ 见表1）相关联的所有的缺点。 在这份报告中，我们报告的制造的SurgiLux的相对简单的协议，并展示其激光激活和组织的熔接强度。 SurgiLux薄膜附着到胶原组织未经化学修饰，如交联和通过使用比较低的供电（120毫瓦）的红外激光，而不是的UV光的照射。壳聚糖膜胶原蛋白（约3千帕）有一个自然，而是弱的附着力，激光活化的壳聚糖基于SurgiLux电影强调了这种粘附强度，通过聚合物链相互作用的作为瞬态热膨胀的结果。没有这一“激活”过程，SurgiLux薄膜容易地除去。6-9 SurgiLux已经过测试，无论是在体外和体内的各种组织，包括神经，肠，硬脑膜和角膜。在所有情况下表现出良好的生物相容性，可以忽略不计热损伤照射的结果。6-10

离心分离导致的透明的绿色溶液，从而增加存储后的粘度在4-6℃下绿色溶液静置3周后，被转换成约20微米厚的透明的绿色SurgiLux膜，显示在视频中，很容易灵活。 一旦照射激光，SurgiLux膜债券到组织。在边缘处的膜组织出现收缩作为激光束通过的膜（ 图2），可以观察到这一点。无炭化或切除组织和薄膜应得到遵守。的接合强度SurgiLux到组织应该足以抬起平分的组?...

Watch this Scientific Journal Video about A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration at JoVE.com

A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration

一种新型的制造，柔性薄膜手术胶粘剂FDA批准的成分，壳聚糖和吲哚青绿。证明本胶原组织的粘接剂具有低功率的红外激光通过一个简单的激活过程的粘合。

基于几丁聚糖，激光激发薄膜手术胶粘剂，“SurgiLux的制备及示范

Sutures are a 4,000 year old technology that remain the 'gold-standard' for wound closure by virtue of their repair strength (~100 KPa). However, sutures ...

Research

JoVE Journal

Bioengineering

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

构建交付的干细胞壳聚糖微球的胶原凝胶

Multipotent stem cells have been shown to be extremely useful in the field of regenerative medicine1-3. However, in order to use these cells effectively ...

科研

生物工程

Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair

激光对生物组织的修复孟加拉玫瑰红壳聚糖膜的制备及应用

Photochemical tissue bonding (PTB) is a sutureless technique for tissue repair, which is achieved by applying a solution of rose bengal (RB) between two ...

Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

创建的胶粘剂和可溶性梯度荧光显微镜成像细胞迁移

Cells can sense and migrate towards higher concentrations of adhesive cues such as the glycoproteins of the extracellular matrix and soluble cues such as ...

Insertion of Flexible Neural Probes Using Rigid Stiffeners Attached with Biodissolvable Adhesive

柔性神经微探针的使用附加与Biodissolvable胶粘剂刚性加劲肋插入

Microelectrode  arrays for neural interface devices that are made of biocompatible thin-film  polymer are expected to have extended functional lifetime ...

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

釉原蛋白 - 壳聚糖水凝胶的发展<em&gt;体外</em&gt;搪瓷愈合了密集接口

Biomimetic enamel reconstruction is a significant topic in material science and dentistry as a novel approach for the treatment of dental caries or ...

Custom-designed Laser-based Heating Apparatus for Triggered Release of Cisplatin from Thermosensitive Liposomes with Magnetic Resonance Image Guidance

定制设计的基于激光的加热装置顺铂触发释放的热敏脂质体与磁共振成像制导

Liposomes have been employed as drug delivery systems to target solid tumors through exploitation of the enhanced permeability and retention (EPR) effect ...

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

自动化脂双层膜的形成使用聚二甲基硅氧烷薄膜

An artificial lipid bilayer, or black lipid membrane (BLM), is a powerful tool for studying ion channels and protein interactions, as well as for ...

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

影像引导下，血管源性微流控网络的基于激光的研制

This detailed protocol outlines the implementation of image-guided, laser-based hydrogel degradation for the fabrication of vascular-derived microfluidic ...

Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

干膜 Photoresist-based 电化学微流控生物传感器平台: 器件制备、片上检测制备及系统运行

In recent years, biomarker diagnostics became an indispensable tool for the diagnosis of human disease, especially for the point-of-care diagnostics. An ...

Rat Model of Adhesive Capsulitis of the Shoulder

肩上粘 Capsulitis 大鼠模型的建立

This proposal aims to create an in vivo rat model of adhesive capsulitis for researching potential treatment options for this condition and other ...