Name: a chitosan based, laser activated thin film surgical adhesive, 'surgilux': preparation and demonstration
Uploaded: 2012-10-23T14:00:00
Description: Watch this Scientific Journal Video about A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration at JoVE.com

लेखकों LJR फोस्टर ऑस्ट्रेलिया के राष्ट्रीय स्वास्थ्य और चिकित्सा अनुसंधान परिषद (NHMRC 1,000,674) से एक अनुदान को स्वीकार करते हैं.

1. SurgiLux समाधान की तैयारी <ol><li> एक 2% एसिटिक एक स्वच्छ कांच बीकर में विआयनीकृत पानी का उपयोग एसिड के समाधान (v / v) तैयार संक्रमण से बचने के लिए एक लामिना का प्रवाह हुड का उपयोग करने के लिए. </li><li> Chromophore के 0.02% (w / v) वजन, हरी ...

<ol>
	<li>Kjaergard, H. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Suture+support:+is+it+advantageous.">Suture support: is it advantageous.</a> Am. J. Surg. 182, 15S-20S (2001).</li><li>Lauto, A., Mawad, D., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adhesive+biomaterials+for+tissue+reconstruction.">Adhesive biomaterials for tissue reconstruction.</a> J. Chem. Tech. Biotech. 83, 464-472 (2008).</li><li>Fung, L. C., Mingin, G. C., Massicotte, M., Felsen, D., Poppas, D. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effects+of+temperature+on+tissue+thermal+injury+and+wound+strength+after+photochemical+wound+closure.">Effects of temperature on tissue thermal injury and wound strength after photochemical wound closure.</a> Lasers Surg. Med. 25, 285-290 (1999).</li><li>Piribo, <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Glues &amp; Sealants: Industry Background Report. , (2005).</li><li>Lauto, A., Hook, J., Doran, M., Camacho, F., Poole-Warren, L. A., Avolio, A., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chitosan+adhesive+for+laser+tissue-welding:+in+vitro+characterisation.">Chitosan adhesive for laser tissue-welding: in vitro characterisation.</a> Lasers Surg. Med. 36, 193-201 (2005).</li><li>Lauto, A., Stoodley, M., Marcel, H., Avolio, A., Sarris, M., McKenzie, G., Sampson, D. D., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+and+in+vivo+tissue+repair+with+laser-activated+chitosan+adhesive.">In vitro and in vivo tissue repair with laser-activated chitosan adhesive.</a> Lasers Surg. Med. 39, 19-27 (2007).</li><li>Lauto, A., Foster, L. J. R., Avolio, A., Sampson, D., Raston, C., Sarris, M., McKenzie, G., Stoodley, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sutureless+Nerve+Repair+with+Laser-Activated+Chitosan+Adhesive:+A+Pilot+in+vivo+Study.">Sutureless Nerve Repair with Laser-Activated Chitosan Adhesive: A Pilot in vivo Study.</a> J. Photomed. Laser. Surg. 26 (3), 227-234 (2008).</li><li>Mar&#231;al, H., Badylak, S. F., Sellaro, T. L., Lauto, A., Foster, L. J. R., Mahler, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+coalescence+of+decellularized+tissue+scaffolds,+laser-activated+chitosan+bioadhesive+and+olfactory+ensheathing+cells+for+tissue+repair+and+regeneration+of+the+spinal+cord.">The coalescence of decellularized tissue scaffolds, laser-activated chitosan bioadhesive and olfactory ensheathing cells for tissue repair and regeneration of the spinal cord.</a> Lasers Med. Sci. 23 (1), 96 (2008).</li><li>Foster, L. J. R., Thomson, K., Marcal, H., Butt, J., Watson, S., Wakefield, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+chitosan-vancomycin+composite+biomaterial+as+a+laser+activated+surgical+adhesive+with+regional+antimicrobial+activity.">A chitosan-vancomycin composite biomaterial as a laser activated surgical adhesive with regional antimicrobial activity.</a> Biomacromolecules. 11 (12), 3563-3570 (2010).</li><li>Shahbazi, J., Marcal, H., Watson, S., Wakefield, D., Sarris, M., Foster, L. J. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sutureless+sealing+of+penetrating+corneal+wounds+using+a+laser-activated+thin+film+adhesive.">Sutureless sealing of penetrating corneal wounds using a laser-activated thin film adhesive.</a> Lasers Surg. Med. , .</li><li>Meyers, M. A., Chen, P. -. Y., Lin, A. Y. -. M., Seki, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biological+materials:+Structure+and+mechanical+properties.">Biological materials: Structure and mechanical properties.</a> Prog. Mater. Sci. 53 (1), 1-206 (2008).</li></ol>

Chitosan आणविक वजन की एक किस्म में और deactylation (डीडीए) के विभिन्न डिग्री के साथ प्राप्त किया जा सकता है. Chitosan शुद्धता में बदलाव SurgiLux समाधान में particulates की उपस्थिति के लिए नेतृत्व कर सकते हैं, centrifugation इन को खत्म करने और एक प?...

<table border="1"><tbody><tr><td> अभिकर्मक / उपकरण के नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियां (वैकल्पिक) </td></tr><tr><td> Chitosan </td><td> सिग्मा Aldrich </td><td> 448877 </td><td></td></tr><tr><td> ग्रीन Indocyanine </td><td> सिग्मा Aldrich </td><td> I2633 </td><td> इसके अलावा Cardiogreen के रूप में जाना जाता है </td></tr><tr><td> शुक्ताम्ल </td><td> सिग्मा Aldrich </td><td> 320099 </td><td></td></tr><tr><td> फाइबर वितरण के साथ इन्फ़रा लाल लेजर डायोड. (808 एनएम, 120 मेगावाट, बीम कोर 200 सुक्ष्ममापी) </td><td> सीएनआई पराबैंगनीकिरण </td><td> एफसी-808 </td><td> अप करने के लिए 5 परिवर्तनीय प्रणाली डब्ल्यू शक्ति </td></tr><tr><td> लेजर सुरक्षा चश्मा </td><td> सीएनआई पराबैंगनीकिरण </td><td> LS-G </td><td></td></tr><tr><td> खिंचनेवाला परीक्षण उपकरण </td><td> Instron Pty लिमिटेड </td><td> 5542 </td><td> 50 एन लोड सेल </td></tr></tbody></table>

a chitosan based, laser activated thin film surgical adhesive, 'surgilux': preparation and demonstration

Sutures are a 4,000 year old technology that remain the 'gold-standard' for wound closure by virtue of their repair strength (~100 KPa). However, sutures can act as a nidus for infection and in many procedures are unable to effect wound repair or interfere with functional tissue regeneration.1 Surgical glues and adhesives, such as those based on fibrin and cyanoacrylates, have been developed as alternatives to sutures for the repair of such wounds. However, current commercial adhesives also have significant disadvantages, ranging from viral and prion transfer and a lack of repair strength as with the fibrin glues, to tissue toxicity and a lack of biocompatibility for the cyanoacrylate based adhesives. Furthermore, currently available surgical adhesives tend to be gel-based and can have extended curing times which limit their application.2 Similarly, the use of UV lasers to facilitate cross-linking mechanisms in protein-based or albumin 'solders' can lead to DNA damage while laser tissue welding (LTW) predisposes thermal damage to tissues.3 Despite their disadvantages, adhesives and LTW have captured approximately 30% of the wound closure market reported to be in excess of US $5 billion per annum, a significant testament to the need for sutureless technology.4
In the pursuit of sutureless technology we have utilized chitosan as a biomaterial for the development of a flexible, thin film, laser-activated surgical adhesive termed 'SurgiLux'. This novel bioadhesive uses a unique combination of biomaterials and photonics that are FDA approved and successfully used in a variety of biomedical applications and products. SurgiLux overcomes all the disadvantages associated with sutures and current surgical adhesives (see Table 1).
In this presentation we report the relatively simple protocol for the fabrication of SurgiLux and demonstrate its laser activation and tissue weld strength. SurgiLux films adhere to collagenous tissue without chemical modification such as cross-linking and through irradiation using a comparatively low-powered (120 mW) infrared laser instead of UV light. Chitosan films have a natural but weak adhesive attraction to collagen (~3 KPa), laser activation of the chitosan based SurgiLux films emphasizes the strength of this adhesion through polymer chain interactions as a consequence of transient thermal expansion.5 Without this 'activation' process, SurgiLux films are readily removed.6-9 SurgiLux has been tested both in vitro and in vivo on a variety of tissues including nerve, intestine, dura mater and cornea. In all cases it demonstrated good biocompatibility and negligible thermal damage as a consequence of irradiation.6-10

केन्द्रापसारण एक पारदर्शी हरी समाधान, ° जो भंडारण के बाद 4-6 चिपचिपाहट बढ़ जाती सी. की ओर जाता है 3 सप्ताह के लिए खड़ा करने के बाद, हरी समाधान एक पारदर्शी हरी SurgiLux लगभग 20 माइक्रोन मोटी फिल्म में परिवर्तित किय?...

Watch this Scientific Journal Video about A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration at JoVE.com

A Chitosan Based, Laser Activated Thin Film Surgical Adhesive, 'SurgiLux': Preparation and Demonstration

एक उपन्यास का निर्माण, एफडीए को मंजूरी दी सामग्री से लचीला पतली फिल्म शल्य चिकित्सा चिपकने वाला, chitosan और हरे रंग indocyanine वर्णित है. एक कम शक्ति लेजर बुनियादी लाल के साथ एक सरल सक्रियण प्रक्रिया के माध्यम से collagenous ऊतकों को इस चिपकने के संबंध में प्रदर्शन किया है.

एक Chitosan आधारित, लेजर सक्रिय पतला फिल्मी सर्जिकल चिपकने वाला, &#39;SurgiLux&#39;: तैयारी और प्रदर्शन

Sutures are a 4,000 year old technology that remain the 'gold-standard' for wound closure by virtue of their repair strength (~100 KPa). However, sutures ...

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