Name: rodent stereotaxic surgery and animal welfare outcome improvements for behavioral neuroscience
Uploaded: 2012-01-30T13:00:00
Description: Watch this Scientific Journal Video about Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience at JoVE.com

Forfatterne takker Dr. Peter Gerrits for de tegninger, der anvendes i videoen.

Bemærk: Antiseptisk teknikker bør anvendes under hele proceduren. Alle instrumenter og materialer (bomuld tippes svaberprøver, gaze, osv.), der vil blive brugt under operationen, bør steriliseres ved autoklavering. En kirurgisk maske, hår motorhjelm og sterile handsker bør anvendes. Arbejdsområdet og stereotaxic apparatet skal rengøres grundigt og desinficeres med en 70% ethanol opløsning. 1. Pre-kirurgiske procedurer <ol><li> Konfigurer stereotaxic apparatur og alle nødvendige m...

<ol>
	<li>Russell, W. M. S., Burch, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Principles of Humane Experimental Technique. , (1959).</li><li>Richardson, C. A., Flecknell, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anaesthesia+and+post-operative+analgesia+following+experimental+surgery+in+laboratory+rodents:+are+we+making+progress?.">Anaesthesia and post-operative analgesia following experimental surgery in laboratory rodents: are we making progress?.</a> Altern. Lab Anim. 33, 119-127 (2005).</li><li>Stokes, E. L., Flecknell, P. A., Richardson, C. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reported+analgesic+and+anaesthetic+administration+to+rodents+undergoing+experimental+surgical+procedures.">Reported analgesic and anaesthetic administration to rodents undergoing experimental surgical procedures.</a> Lab Anim. 43, 149-154 (2009).</li><li>Flecknell, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Laboratory Animal Anaesthesia - A Practical Introduction for Research Workers and Technicians. , (2009).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=DIRECTIVE+2010/63/EU+OF+THE+EUROPEAN+PARLIAMENT+AND+OF+THE+COUNCIL+of+22+September+2010+on+the+protection+of+animals+used+for+scientific+purposes.">DIRECTIVE 2010/63/EU OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 22 September 2010 on the protection of animals used for scientific purposes.</a> Official Journal of the European Union. , (2010).</li><li>Geiger, B. M., Frank, L. E., Caldera-Siu, A. D., Pothos, E. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Survivable+Stereotaxic+Surgery+in+Rodents.">Survivable Stereotaxic Surgery in Rodents.</a> J. Vis. Exp. (20), e880-e880 (2008).</li><li>Hellebrekers, L. J., Booij, L. H. D. J., Flecknell, P. A., Van Zutphen, L. F. M., Baumans, V., Beynen, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Anaesthesia,+analgesia+and+euthanasia.">Anaesthesia, analgesia and euthanasia.</a> Principles of Laboratory Animal Science. , 277-311 (2001).</li><li>Walantus, W., Elias, L., Kriegstein, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+utero+intraventricular+injection+and+electroporation+of+E16+rat+embryos.">In utero intraventricular injection and electroporation of E16 rat embryos.</a> J. Vis. Exp. (6), e236-e236 (2007).</li><li>Paxinos, G., Watson, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Rat Brain in Stereotaxic Coordinates. , (1986).</li><li>Paxinos, G., Watson, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Rat Brain in Stereotaxic Coordinates. , (2007).</li><li>Swanson, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Brain Maps: Structure of the Rat Brain. , (1992).</li></ol>

Hovedformålet med denne video præsentation er at gøre adfærdsmæssige neuroforskere med de grundlæggende principper for stereotaxic kirurgi. Forskere, der allerede klarer stereotaxic kirurgi kan også drage fordel af denne video og overveje nogle af de proceduremæssige justeringer til brug i deres eget laboratorium. En stadig stigende viden om optimering af kirurgiske teknikker 1-3, udvikling af nye anæstetika og analgetika til brug i human-og veterinærmedicin 4,12, den offentlige bevidsthe...

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rodent stereotaxic surgery and animal welfare outcome improvements for behavioral neuroscience

Stereotaxic kirurgi for implantation af kanyler i bestemte områder af hjernen har i mange årtier været en meget vellykket eksperimentel teknik til at undersøge virkningerne af lokalt manipuleret neurotransmitter og signalveje i vågen, opfører dyr. Desuden har stereotaxic implantation af elektroder til elektrofysiologiske stimulering og optagelse undersøgelser været medvirkende til vores nuværende forståelse af neuroplasticitet og hjerne netværk i opfører dyr. Stadig større viden om optimering af kirurgiske teknikker i gnavere 1-4, den offentlige bevidsthed om dyrevelfærd og strengere lovgivning (fx i 2010 EU direktiv om anvendelse af forsøgsdyr 5) fået os til at finpudse disse kirurgiske procedurer, især med hensyn at gennemføre nye procedurer for oxygensupplement og løbende overvågning af blodets iltning og puls niveauer under operationen samtat indføre en standardiseret protokol for post-kirurgisk behandling. Vores observationer tyder på, at disse ændringer resulterede i en øget overlevelsesrate og en forbedring af den generelle tilstand af dyrene efter operationen (f.eks mindre vægttab og en mere aktiv dyr). Denne video præsentation vil vise de generelle procedurer, der er involveret i denne type stereotaxic kirurgi med særlig vægt på vores flere ændringer. Vi vil illustrere disse kirurgiske procedurer hos rotter, men det er også muligt at udføre denne type kirurgi i mus eller andre små forsøgsdyr ved at anvende særlige adaptere for stereotaxic apparatet 6.

Watch this Scientific Journal Video about Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience at JoVE.com

Rodent Stereotaxic Surgery and Animal Welfare Outcome Improvements for Behavioral Neuroscience

Stereotaxic kirurgi på gnavere giver mulighed for målrettet administration af lægemidler eller elektrisk stimulation og optagelser i vågen, opfører dyr. I denne video præsentation vil vi vise de seneste proceduremæssige justeringer af denne langvarige procedure, der med succes forbedret overlevelse sats og reduceret post-operative vægttab.

Gnaver Stereotaxic Kirurgi og Trivsel Outcome Forbedringer for Behavioral Neuroscience

Stereotaxic surgery for the implantation of cannulae into specific brain regions has for many decades been a very successful experimental technique to ...

Research

JoVE Journal

Neuroscience

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