Name: cutaneous leishmaniasis in the dorsal skin of hamsters: a useful model for the screening of antileishmanial drugs
Uploaded: 2012-04-21T14:00:00
Description: Watch this Scientific Journal Video about Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs at JoVE.com

Denne undersøgelse blev støttet af tilskud fra Udvalget for forskning på University of Antioquia (CODI), Den colombianske institut for udvikling af videnskab og teknologi - COLCIENCIAS og Center for Udvikling af produkter mod tropesygdomme - CIDEPRO.

1. Infektion af hamstere 1. Dyr Indavlede kvindelige og mandlige guldhamstere (Mesocricetus auratus), 6-8 uger, der vejer 140-160 g anvendes. De huse på dyret anlægget i temperaturstyret indkvartering, fodret med standard gnavere tørret mad og forsynet med vand ad libitum. Alle de procedurer, der involverer dyr er godkendt af den institutionelle Etisk Komité for Experimental Animal Use. Før eksperimentel infektion med dermotropic Leishmania<...

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Kutan leishmaniasis er endemisk i troperne og nye verden. Det er ofte omtalt som en gruppe af sygdomme på grund af varierende spektrum af kliniske manifestationer, der spænder fra små kutane noduler grov slimhindevæv ødelæggelse. Mest tilgængelige lægemidler er dyre, kræver lange behandlingsforløb og bliver stadig ineffektive, hvilket nødvendiggør opdagelsen af ​​nye lægemidler. Musemodellen er almindeligt anvendt, men har nogle ulemper. Således for eksempel på grundlag af...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Type </td><td> Firma </td><td> Katalog nummer </td></tr><tr><td> Ketamin </td><td> Reagens </td><td> Holliday-Scott SA </td><td></td></tr><tr><td> Xilacine </td><td> Reagens </td><td> Syntese </td><td></td></tr><tr><td> PBS </td><td> Reagens </td><td> GIBCO </td><td> 14190-136 </td></tr><tr><td> Digital skydelære </td><td> Udstyr </td><td> Fisher </td><td> 15-077-958 </td></tr><tr><td> Giemsa </td><td> Reagens </td><td> SIGMA </td><td> GS1L-1L </td></tr><tr><td> Formalin </td><td> Reagens </td><td> Nova lab </td><td> 11273 </td></tr><tr><td> Paraffin </td><td> Reagens </td><td> Pechiney Plastemballage </td><td> PM-996 </td></tr><tr><td> Haemotoxilin </td><td> Reagens </td><td> Nova lab </td><td&gt; 10870103 </td></tr><tr><td> Eosin </td><td></td><td> Nova lab </td><td> 10870203 </td></tr><tr><td> Kodak Ektachem tørkemi </td><td> Udstyr </td><td> Kodak Ektachem </td><td> DT-60 </td></tr><tr><td> meglumin antimoniate </td><td> Reagens </td><td> Aventis </td><td></td></tr><tr><td> Mikroskopi </td><td> Udstyr </td><td> Nikon </td><td> YS2-T </td></tr></tbody></table>

cutaneous leishmaniasis in the dorsal skin of hamsters: a useful model for the screening of antileishmanial drugs

Traditionelt hamstere eksperimentelt inokuleret i snuden eller trædepuden. Imidlertid i disse sites et sår ikke altid forekommer, måling af læsionsstørrelse er en hård procedure og dyr viser vanskeligt at spise, ånde og bevæge sig på grund af læsionen. For at optimere hamster-model for kutan leishmaniasis, unge voksne mandlige og kvindelige guldhamstere (Mesocricetus auratus) blev injiceret intradermalt på den dorsale hud med 1 til 1,5 x L0 7 promastigoter af Leishmania arter og progression af efterfølgende læsioner blev evalueret i op til 16 uger efter infektion. Den gyldne hamster blev valgt, fordi det anses for passende bio-model til evaluering af lægemidler mod Leishmania, som de er modtagelige for infektion med forskellige arter. Kutan infektion af hamstere resulterer i kroniske, men kontrolleret læsioner, og et klinisk udvikling med tegn svarende til dem observeret hos mennesker. Derfor, etablering of omfanget af infektion ved måling af størrelsen af ​​læsionen ifølge området indurations og sår er mulig. Denne fremgangsmåde har bevist sin alsidighed og nem håndtering under podning, opfølgning og karakterisering af typiske læsioner (sår), anvendelse af behandlinger gennem forskellige måder og opnåelse af kliniske prøver efter forskellige behandlinger. Ved hjælp af denne metode kvaliteten af ​​dyrelivet med hensyn til bevægelse, søge efter mad og vand, leg og sociale aktiviteter isalso bevaret.

Watch this Scientific Journal Video about Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs at JoVE.com

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Optimering af den eksperimentelle hamster-model for kutan leishmaniasis ved intradermal injektion af<em&gt; Leishmania</em&gt; Promastigoter i den dorsale hud. Denne fremgangsmåde er nyttig under inokulation, opfølgning, karakterisering af læsioner, anvendelse af behandlinger og opnåelse af kliniske prøver. Locomotion, søge efter mad og vand, leg og sociale aktiviteter bevaret.

Kutan leishmaniasis i den dorsale hud af hamstere: en nyttig model til screening af Antileishmanial Drugs

Traditionally, hamsters are experimentally inoculated in the snout or the footpad. However in these sites an ulcer not always occurs, measurement of ...

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