Name: cutaneous leishmaniasis in the dorsal skin of hamsters: a useful model for the screening of antileishmanial drugs
Uploaded: 2012-04-21T14:00:00
Description: Watch this Scientific Journal Video about Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs at JoVE.com

Denne studien ble støttet med tilskudd fra Utvalget for forskning ved University of Antioquia (CODI), Den colombianske Institutt for utviklingen av vitenskap og teknologi - COLCIENCIAS og Senter for utvikling av produkter mot tropiske sykdommer - CIDEPRO.

1. Infeksjon av hamstere 1. Dyr Innavlede kvinnelige og mannlige gylne hamstere (Mesocricetus auratus), 6-8 uker, veier 140-160 g er brukt. De ligger på dyret anlegget, i temperaturkontrollerte innkvartering, matet med standard gnager tørket mat og utstyrt med vann ad libitum. Alle prosedyrer som involverer dyr blir godkjent av institusjonelle Etisk komité for Experimental Animal bruk. Før eksperimentell infeksjon med dermotropic Leishmania</...

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Kutan leishmaniasis er endemisk i tropene og neotropics. Det blir ofte referert til som en gruppe av sykdommer på grunn av den varierte spekter av kliniske manifestasjoner, som spenner fra små kutane knuter til brutto slimhinnene vev ødeleggelse. De fleste tilgjengelige medikamenter er kostbare, krever lange behandlingsregimer og blir stadig ineffektivt, nødvendiggjør oppdagelsen av nye legemidler. Musen Modellen er mye brukt, men har noen ulemper. Dermed for eksempel på grunnlag av le...

<table border="1"><tbody><tr><td> Navn på reagensen </td><td> Type </td><td> Firma </td><td> Katalognummer </td></tr><tr><td> Ketamin </td><td> Reagens </td><td> Holliday-Scott SA </td><td></td></tr><tr><td> Xilacine </td><td> Reagens </td><td> Synthesis </td><td></td></tr><tr><td> PBS </td><td> Reagens </td><td> GIBCO </td><td> 14190-136 </td></tr><tr><td> Digital caliper </td><td> Utstyr </td><td> Fisher </td><td> 15-077-958 </td></tr><tr><td> Giemsa </td><td> Reagens </td><td> SIGMA </td><td> GS1L-1L </td></tr><tr><td> Formalin </td><td> Reagens </td><td> Nova lab </td><td> 11273 </td></tr><tr><td> Parafin </td><td> Reagens </td><td> Pechiney plast emballasje </td><td> PM-996 </td></tr><tr><td> Haemotoxilin </td><td> Reagens </td><td> Nova lab </td><td&gt; 10870103 </td></tr><tr><td> Eosin </td><td></td><td> Nova lab </td><td> 10870203 </td></tr><tr><td> Kodak Ektachem tørr kjemi </td><td> Utstyr </td><td> Kodak Ektachem </td><td> DT-60 </td></tr><tr><td> meglumin antimoniate </td><td> Reagens </td><td> Aventis </td><td></td></tr><tr><td> Mikroskopi </td><td> Utstyr </td><td> Nikon </td><td> YS2-T </td></tr></tbody></table>

cutaneous leishmaniasis in the dorsal skin of hamsters: a useful model for the screening of antileishmanial drugs

Tradisjonelt er hamstere eksperimentelt inokulert i trynet eller footpad. Men i disse områdene en sår ikke alltid skjer, er måling av lesjon størrelse en hard prosedyre og dyr viser problemer med å spise, puste og bevege på grunn av lesjon. For å optimalisere hamster modell for kutan leishmaniasis, ung voksen mann og kvinnelige gylne hamstere (Mesocricetus auratus) ble injisert intradermalt på dorsal huden med 1 til 1,5 x l0 7 promastigotes av Leishmania arter og progresjon av påfølgende lesjonene ble evaluert for inntil 16 uker etter smitte. Den gylne hamster ble valgt fordi det anses tilstrekkelig bio-modell for å evaluere legemidler mot Leishmania som de er utsatt for smitte av ulike arter. Kutan infeksjon av hamstere resulterer i kroniske men kontrollert lesjoner, og en klinisk utvikling med symptomer som ligner på de som er observert hos mennesker. Derfor, etablering of omfanget av smitte ved å måle størrelsen på lesjonen i henhold til området av indurasjon og sår er gjennomførbart. Denne tilnærmingen har vist sin allsidighet og enkel administrasjon under inokulasjon, oppfølging og karakterisering av typiske lesjoner (magesår), anvendelse av behandlinger gjennom ulike måter og innhenting av kliniske prøver etter ulike behandlinger. Ved å bruke denne metoden kvaliteten på dyrelivet om bevegelse, søker etter mat og vann, lek og sosiale aktiviteter isalso bevart.

Watch this Scientific Journal Video about Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs at JoVE.com

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Optimalisering av den eksperimentelle hamster modell for kutan leishmaniasis av intradermal injeksjon av<em&gt; Leishmania</em&gt; Promastigotes på dorsal huden. Denne tilnærmingen er nyttig under inokulasjon, oppfølging, karakterisering av lesjoner, anvendelse av behandlinger og innhenting av kliniske prøver. Bevegelse, søker etter mat og vann, er lek og sosiale aktiviteter bevart.

Kutan Leishmaniasis i Dorsal Skin av Hamstere: en Nyttig Modell for Screening av Antileishmanial narkotika

Traditionally, hamsters are experimentally inoculated in the snout or the footpad. However in these sites an ulcer not always occurs, measurement of ...

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