Name: cutaneous leishmaniasis in the dorsal skin of hamsters: a useful model for the screening of antileishmanial drugs
Uploaded: 2012-04-21T14:00:00
Description: Watch this Scientific Journal Video about Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs at JoVE.com

COLCIENCIAS ve Tropikal Hastalıklar karşı Ürünleri Geliştirme Merkezi - - CIDEPRO Bu çalışma Antioquia Üniversitesi (Codi), Fen ve Teknoloji Geliştirme Kolombiyalı Enstitüsü Araştırma Komitesi hibe ile desteklenmiştir.

1. Hamsterlerin Enfeksiyon 1. Hayvanlar 140-160 g ağırlığında kendilenmiş kadın ve erkek altın hamster (Mesocricetus auratus), 6-8 hafta kullanılır. Onlar standart kemirgen kuru gıda ile beslenen ve su ad libitum ile sağlanan, ısı kontrollü konaklama, hayvan tesiste yer alır. Hayvanları kapsayan tüm işlemler Deney Hayvanları Kullanım kurumsal Etik Komitesi tarafından onaylanmıştır. Dermotropic Leishmania parazitleri ...

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Kutanöz leishmaniasis tropik ve neotropics endemiktir. Genellikle küçük olduğundan kütanöz nodüllerden gros mukozal doku tahribine arasındadır klinik belirtileri, bir değişken spektrumunun bir grup hastalığı olarak adlandırılır. En mevcut ilaçlar pahalı, uzun tedavi rejimleri gerektiren ve yeni ilaçların keşfi gerektiren, giderek etkisiz hale gelmektedir. Fare modelinde yaygın olarak kullanılan ama bazı dezavantajlara sahiptir. Oldukça BALB / c görüldüğü gibi ...

<table border="1"><tbody><tr><td> Reaktif Adı </td><td> Tip </td><td> Şirket </td><td> Katalog numarası </td></tr><tr><td> Ketamin </td><td> Reaktif </td><td> Holliday-Scott SA </td><td></td></tr><tr><td> Xilacine </td><td> Reaktif </td><td> Sentez </td><td></td></tr><tr><td> PBS </td><td> Reaktif </td><td> GIBCO </td><td> 14190-136 </td></tr><tr><td> Dijital kumpas </td><td> Ekipman </td><td> Balıkçı </td><td> 15-077-958 </td></tr><tr><td> Giemsa </td><td> Reaktif </td><td> SIGMA </td><td> GS1L-1L </td></tr><tr><td> Formalin </td><td> Reaktif </td><td> Nova laboratuvarı </td><td> 11273 </td></tr><tr><td> Parafin </td><td> Reaktif </td><td> PECHINEY Plastik Ambalaj </td><td> PM-996 </td></tr><tr><td> Haemotoxilin </td><td> Reaktif </td><td> Nova laboratuvarı </td><td&gt; 10870103 </td></tr><tr><td> Eosin </td><td></td><td> Nova laboratuvarı </td><td> 10870203 </td></tr><tr><td> Kodak Ektachem kuru kimya </td><td> Ekipman </td><td> Kodak Ektachem </td><td> DT-60 </td></tr><tr><td> meglumin antimoniate </td><td> Reaktif </td><td> Aventis </td><td></td></tr><tr><td> Mikroskopi </td><td> Ekipman </td><td> Nikon </td><td> YS2-T </td></tr></tbody></table>

cutaneous leishmaniasis in the dorsal skin of hamsters: a useful model for the screening of antileishmanial drugs

Geleneksel olarak, hamster deneysel burun veya haydut olarak aşılanır. Ancak bu sitelerde bir ülser her zaman gerçekleşir değil, lezyon büyüklüğü ölçümü zor bir işlemdir ve hayvanlar nedeniyle lezyonun, yemek nefes ve hareket zorluğu gösterir. Kutanöz leishmaniasis, genç erişkin erkek ve kadın için altın hamster hamster modeli optimize etmek için (Mesocricetus auratus) 1 ile 1,5 x l0 7 Leishmania türlerinin promastigotları ve daha sonraki lezyonların ilerlemesi ile sırt derisine intradermal enjekte edildi kadar değerlendirildi 16 hafta sonrası enfeksiyon. Farklı türleri tarafından enfeksiyona duyarlı olarak Leishmania karşı ilaçları değerlendirmek için biyo-modeli yeterli kabul edilir çünkü altın hamsteri seçildi. Kronik fakat kontrollü lezyonlar ve insanlarda görülen benzer bulguları ile klinik bir evrim hamster sonuçlarının deri enfeksiyonu. Bu nedenle, o kurulmasısertleştirmek ve ülserlerin alanına göre lezyonun boyutu ölçerek enfeksiyon f ölçüde mümkündür. Bu yaklaşım, aşılama sırasında, çok yönlülüğü ve kolay yönetim kanıtlamış, farklı tedavilerden sonra klinik örneklerin farklı yolları ve alınması yoluyla Tipik lezyonlar (ülserler), tedavilerin uygulama takip ve karakterizasyonu. Bu yöntem hareket ile ilgili hayvan yaşam kalitesi, gıda ve su, oyun ve isalso korunmuş sosyal faaliyetler için arama kullanarak.

Watch this Scientific Journal Video about Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs at JoVE.com

Cutaneous Leishmaniasis in the Dorsal Skin of Hamsters: a Useful Model for the Screening of Antileishmanial Drugs

Intradermal enjeksiyonu ile kutanöz leishmaniasis için deneysel Hamster model Optimizasyonu<em&gt; Leishmania</em&gt; Dorsal deriyi promastigotları. Bu yaklaşım, aşılama sırasında, takip, lezyon karakterizasyonu, tedavilerin uygulama ve klinik örneklerin alınması yararlıdır. Yiyecek ve su için harekettir, arama, oyun ve sosyal aktiviteler korunur.

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Traditionally, hamsters are experimentally inoculated in the snout or the footpad. However in these sites an ulcer not always occurs, measurement of ...

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