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12.2K Views
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09:29 min
December 14th, 2011
DOI :
10.3791/3536-v
Chapters
0:05
Title
1:35
Sample Preparation
2:42
Isoelectric Focusing
4:17
SDS Polyacrylamide Gel Electrophoresis
5:38
In-gel Tryptic Digestion
7:38
Results: Skeletal Muscle Protein
9:08
Conclusion
Summary
一个直接的方法设置隔离并确定在骨骼肌中表达最丰富的蛋白质的身份。从10毫克肌肉的二维凝胶约800点,发现了这样的性别特定蛋白表达的决心。这些方法将给予在相同的结果,大多数组织。
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