Smith College

3 ARTICLES PUBLISHED IN JoVE

Biology

Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation

Elizabeth R. Deschene¹, Michael J. Barresi¹

¹Department of Biological Sciences, Smith College

Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.

Medicine

Skeletal Muscle Gender Dimorphism from Proteomics

Kalina Dimova¹, Lauren Ann Metskas², Mohini Kulp³, Stylianos P. Scordilis⁴

¹Center for Proteomics, Smith College, ²Department of Molecular Biophysics and Biochemistry, Yale University, ³Department of Chemistry, Smith College, ⁴Department of Biological Sciences and Center for Proteomics, Smith College

A straight-forward set of methods to isolate and determine the identity of the most abundant proteins expressed in skeletal muscle. About 800 spots are discerned on a two-dimensional gel from 10 mg muscle; this allows for the determination of gender-specific protein expression. These methods will give equivalent results in most tissues.

Biochemistry

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Jingjie Hu¹, Nathan D. Derr^1,2

¹Department of Biological Sciences, Smith College, ²Center for Microscopy and Imaging, Smith College

The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.