Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Please note that all translations are automatically generated. Click here for the English version.
DOI :
10.3791/3550-v
•
16:33 min
October 22nd, 2011
Chapters
0:03
Title
3:00
Step 1 Making horizontal hippocampal/cortical slices from young mouse brain
5:57
Step 2 Preparation of the staining chamber
8:48
Step 3 Staining of slices
10:21
Step 4 Non-neuronal dyes and older tissue
12:02
Step 5 Attaching slice to recording chamber
13:36
Step 6 Imaging
15:58
Conclusion
神经网络发展的自发活动可以使用AM酯钙敏感的指标染料形式。神经元的激活,细胞内钙的变化,说明被检测为指标荧光瞬态变化与一个或两个光子成像。此协议可适应的发展依赖的神经元网络的范围在体外。
Tags
-- Views
Related Videos
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Overview
Publishing Process
Editorial Board
Scope and Policies
Peer Review
FAQ
Submit
Librarians
Testimonials
Subscriptions
Access
Resources
Library Advisory Board
ABOUT JoVE
Leadership
Copyright © 2024 MyJoVE Corporation. All rights reserved