Name: creating rigidly stabilized fractures for assessing intramembranous ossification, distraction osteogenesis, or healing of critical sized defects
Uploaded: 2012-04-11T12:00:00
Description: Watch this Scientific Journal Video about Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects at JoVE.com

这项工作是由NIAMS以R01-AR053645。

所有的程序批准由加州大学旧金山分校的机构动物照顾及使用委员会，并符合国家准则。 1。手术前准备的固定器<ol><li>前创造稳定骨折，需要装配的定制设计的外固定器。定制设计的外固定帧由两个铝环由三个不锈钢钢＃0/56螺纹棒，六角螺母8＃2/56，17（一小部分公司，佛罗里达州迈阿密湖3）匹配的螺栓稳定。 </li><li>每骨折，需要两个铝环。一环组装六角螺母4＃2/56...

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两种不同的方式取决于机械稳定性（综述：14）骨头愈合。当留在不稳定的一个大型软骨模板形式取代骨骨折的差距，以弥合断骨两端。近端和远端断裂，骨内，骨膜及骨内膜的膜内骨化形成直接的。相比之下，发生在稳定骨折愈合完全通过膜内骨化3。然而，这两个进程之间的开关调节的具体机制不明。有证据表明，机械环境中的干细胞的命运是基因控制，并可以改变。在小鼠体...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td> 0.25毫米昆虫针</td><td>精细科学工具</td><td> 26000-25 </td><td>涂黑阳极氧化钢，0.25毫米杆直径，4厘米的长度</td></tr><tr><td>不锈钢六角螺母</td><td>小零件</td><td> ＃2-56 </td><td> 1/8“的长度，每英寸56线程</td></tr><tr><td>不锈钢六角螺母</td><td>小零件</td><td> ＃0-80 </td><td> 1/8“的长度，每英寸80线程</td></tr><tr><td>不锈钢机螺钉</td><td>小零件</td><td> ＃0-80 </td><td> 1/8“的长度，每英寸80线程</td></tr><tr><td>不锈钢机剪切螺杆</td><td>小零件</td><td> ＃0-80 </td><td> 6“的长度，每英寸80线程</td></tr><tr><td> 18-8不锈钢头机SCREW </td><td>麦克马斯特，卡尔</td><td></td><td> 2-56主题，3/6“的长度</td></tr><tr><td>外固定器</td><td>车间</td><td>定制设计</td><td></td></tr></tbody></table>

creating rigidly stabilized fractures for assessing intramembranous ossification, distraction osteogenesis, or healing of critical sized defects

评估骨骼修复的模式是用于开发用于临床治疗骨折的治疗至关重要。机械稳定性骨损伤愈合中起着很大的作用。在最坏的情况下，机械不稳定，可能导致延迟或在非工会的人。然而，运动还可以促进愈合过程。在骨折有运动软骨组织形式，以稳定骨折的骨端，骨和软骨逐渐取代通过软骨内骨化发育过程的概括。相反，如果骨折稳定硬性骨形成直接通过膜内骨化。临床上，同时出现软骨内和膜内骨化。为了有效地复制这个过程中研究者一根针插入髓腔的断骨Bonnarens 4的 。这种实验方法提供了极好的横向稳定性，同时允许rotatio坚持信号不稳定。然而，我们理解的机制，规范这两个不同的过程，也可以加强实验隔离这些进程。我们已经开发出一种稳定的协议，提供旋转和横向稳定。在这种模式下，膜内骨化是唯一的模式，观察愈合，愈合的参数，可以在不同品系的转基因小鼠5-7后，8,9生物活性分子的应用，改变生理参数的愈合10后，比较，修改后牵张12后的金额或11稳定时间，创 ​​造一个非工会13后，或之后创建一个关键尺寸的缺陷。在这里，我们说明如何申请学习和小鼠胫骨骨折愈合牵张改良Ilizarov外固定器。

Watch this Scientific Journal Video about Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects at JoVE.com

Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects

本文介绍了一种稳定的长骨骨折的方法是基于改良Ilizarov外固定器的应用<sup&gt; 1-3</sup&gt;。固定架和创造的骨损伤的应用后，可以评估愈合，牵张可以被执行，或者可以创建和非工会或大小的关键缺陷，用于研究治疗干预。

建立评估膜内骨化硬性稳定骨折，牵张成骨，或临界中小企业缺损愈合

Assessing modes of skeletal repair is essential for developing therapies to be used clinically to treat fractures. Mechanical stability plays a large role ...

Research

JoVE Journal

Medicine

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修复的临界大小的颅骨缺损模型脂肪间充质干细胞的收获Lipoaspirate

Craniofacial skeletal repair and regeneration offers the promise of de novo tissue formation through a cell-based approach utilizing stem cells. ...

科研

医学

Murine Model of Wound Healing

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Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. ...

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局部或全身药物干预后评估的变化小鼠中挥发性全身麻醉药敏感性

One desirable endpoint of general anesthesia is  the state of unconsciousness, also known as hypnosis. Defining the hypnotic state in animals is  less ...

A Simple Critical-sized Femoral Defect Model in Mice

一个简单的临界尺寸股骨缺损模型小鼠

While bone has a remarkable capacity for regeneration, serious bone trauma often results in damage that does not properly heal. In fact, one tenth of all ...

Direct Mouse Trauma/Burn Model of Heterotopic Ossification

异位骨化直接鼠标创伤/烧伤模型

Heterotopic ossification (HO) is the formation of bone outside of the skeleton which forms following major trauma, burn injuries, and orthopaedic surgical ...

Establishment of a Segmental Femoral Critical-size Defect Model in Mice Stabilized by Plate Osteosynthesis

在小鼠节段性股临界尺寸缺陷模型的建立接骨板稳定

The use of tissue-engineered bone constructs is an appealing strategy to overcome drawbacks of autografts for the treatment of massive bone defects. As a ...

Scleral Cross-linking Using Riboflavin and Ultraviolet-A Radiation for Prevention of Axial Myopia in a Rabbit Model

巩膜交联兔模型使用核黄素和紫外辐射的轴性近视防治

Myopic individuals, especially those with severe myopia, are at higher-than-normal risk of cataract, glaucoma, retinal detachment and chorioretinal ...

The Generation of Closed Femoral Fractures in Mice: A Model to Study Bone Healing

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Bone fractures impose a tremendous socio-economic burden on patients, in addition to significantly affecting their quality of life. Therapeutic strategies ...

Fracture Apparatus Design and Protocol Optimization for Closed-stabilized Fractures in Rodents

鼠类闭合稳定骨折的断裂装置设计与协议优化

The reliable generation of consistent stabilized fractures in animal models is essential for understanding the biology of bone regeneration and developing ...

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

创面愈合研究的前活体角膜器官培养模型

The cornea has been used extensively as a model system to study wound healing. The ability to generate and utilize primary mammalian cells in two ...