Name: creating rigidly stabilized fractures for assessing intramembranous ossification, distraction osteogenesis, or healing of critical sized defects
Uploaded: 2012-04-11T12:00:00
Description: Watch this Scientific Journal Video about Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects at JoVE.com

Dette arbejde blev finansieret af R01-AR053645 fra NIAMS.

Alle procedurer blev godkendt af UCSF Institutional Animal Care og Brug Udvalg og i overensstemmelse med nationale retningslinjer. 1. Fremstillinq af fikseringsindretninger Før kirurgi <ol><li> Før du opretter stabiliseret fraktur, skal en til at samle den brugerdefinerede design ekstern fiksering enhed. De specialdesignede eksterne fiksering rammer bestod af to aluminiums ringe er stabiliseret af tre rustfrit stål # 0/56 gevindstænger, 8 # 2/56 sekskantede møtrikker, og 17 matchende bol...

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Bones helbrede ved to forskellige modaliteter afhængig af mekanisk stabilitet (revideret i: 14). Da forlod ustabile en stor brusk skabelon former i frakturhullet, der erstattes af knogle til at bygge bro de to ender af brækkede knogler. Proksimalt og distalt til pausen, knogler danner direkte ved intramembranous ossifikation i periost og endosteum. I modsætning hertil forekommer i stabile frakturer heling udelukkende via intramembranous knogledannelse 3. De specifikke mekanismer, der regulerer s...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalog nummer </td><td> Kommentarer </td></tr><tr><td> 0,25 mm insekt pin </td><td> Fin Science Tool </td><td> 26000-25 </td><td> Blacked anodized, 0,25 mm stang diameter, 4 cm længde </td></tr><tr><td> Rustfrit stål Hex Nut </td><td> Små dele </td><td> # 2-56 </td><td> 1/8 &quot;længde 56 tråde per inch </td></tr><tr><td> Rustfrit stål Hex Nut </td><td> Små dele </td><td> # 0-80 </td><td> 1/8 &quot;længde 80 tråde per inch </td></tr><tr><td> Rustfrit stål Maskinskrue </td><td> Små dele </td><td> # 0-80 </td><td> 1/8 &quot;længde 80 tråde per inch </td></tr><tr><td> Rustfrit stål Machine Cut gevind Rod </td><td> Små dele </td><td> # 0-80 </td><td> 6 &quot;længde 80 tråde per inch </td></tr><tr><td> 18-8 rustfrit stål hoved Machine ScREW </td><td> McMaster-Carr </td><td></td><td> 2-56 Tråde, 3/6 &quot;længde </td></tr><tr><td> Ekstern fikseringsanordning </td><td> Machine Shop </td><td> Custom-designet </td><td></td></tr></tbody></table>

creating rigidly stabilized fractures for assessing intramembranous ossification, distraction osteogenesis, or healing of critical sized defects

Vurdering tilstande knogler reparation er nødvendig for at udvikle terapier, der skal anvendes klinisk til behandling af frakturer. Mekanisk stabilitet spiller en stor rolle i heling af knoglelæsioner. I værste fald mekanisk ustabilitet kan føre til forsinkede eller ikke-union i mennesker. Men, kan bevægelse også stimulere helingsprocessen. I frakturer, der har bevægelse brusk formularer for at stabilisere bruddet knogleenderne, og denne brusk gradvist erstattes af knogle gennem sammenfatning af den udviklingsmæssige proces endochondral ossifikation. I modsætning hertil knogle hvis en knoglefraktur er stift stabiliserede former direkte via intramembranous knogledannelse. Klinisk, både endochondral og intramembranous ossifikation sker samtidigt. Til effektivt at gentage denne proces undersøgere indsætte en stift i marvkanalen i den brækkede knogle, som beskrevet af Bonnarens 4. Denne eksperimentelle fremgangsmåde tilvejebringer fremragende lateral stabilitet, samtidig med at rotatioNal ustabilitet til at fortsætte. Dog kan vores forståelse af de mekanismer, der regulerer disse to forskellige processer også blive styrket ved eksperimentelt at isolere hver af disse processer. Vi har udviklet en stabilisering protokol, der giver roterende og lateral stabilisering. I denne model er intramembranous ossifikation den eneste form for healing, der er observeret, og healing parametre kan sammenlignes mellem forskellige stammer af genetisk modificerede mus 5-7, efter anvendelse af bioaktive molekyler 8,9, efter at ændre fysiologiske parametre healing 10, efter at ændre mængden eller tidspunktet for stabilisering 11, efter distraktion osteogenesis 12, og efter oprettelsen af et ikke-union 13, eller efter oprettelse af en kritisk størrelse defekt. Her viser vi hvordan du anvender de modificerede Ilizarov fikseringsindretninger for at studere tibiale frakturheling og distraktion osteogenese i mus.

Watch this Scientific Journal Video about Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects at JoVE.com

Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects

Denne artikel beskriver en fremgangsmåde til stabilisering lange knoglefrakturer, som er baseret på anvendelsen af ​​modificerede Ilizarov ydre fikseringsindretninger<sup&gt; 1-3</sup&gt;. Efter påføring af fikseringsindretninger og skabelse af knoglen skade, kan healing vurderes, kan distraktion osteogenesis udføres, eller ikke-union eller kritiske størrelse defekt kan oprettes og bruges til at studere terapeutiske indgreb.

Oprettelse af stift Stabiliserede Brud for vurdering Intramembranous ossifikation, Distraktion Osteogenesis eller Healing af Kritiske Sized Mangler

Assessing modes of skeletal repair is essential for developing therapies to be used clinically to treat fractures. Mechanical stability plays a large role ...

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