Name: mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model
Uploaded: 2012-03-13T13:00:00
Description: Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

笔者想感谢他们的帮助与照顾动物肖纳赖德奥特格罗斯和亚历山德拉Garces，平吴为她的技术援助。由国立卫生研究院下，没有为这项工作提供了支持。 EB003268，加拿大研究主席的计划。

1。超声和​​MRI设置在这项研究中使用了核磁共振兼容的三轴聚焦超声系统（聚焦超音波仪器公司，多伦多，安大略，加拿大）。使用两种不同的超声换能器：内部构造551.5千赫球为中心的传感器（曲率半径为60毫米，外直径为75毫米，内直径为20毫米），和1.503兆赫，8部门阵列综合作为一个单一的元素球聚焦换能器（FN = 0.8，光圈= 10厘米）驱动压电陶瓷水听器（Imasonic公司，Vo...

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动物和微泡的制备此过程中最关键的环节。必须完全去除头发上的动物的头，以避免衰减的超声波束。血脑屏障可以isofluorane麻醉下打乱，但是，它变得更加难以实现一致的开幕式。 应始终与保健和小计处理的微气泡，大口径的针头必须使用绘图时，为了避免打破他们。同样，可以合理地用在尾静脉最小的压力表导管应采用建议（22号）。如果需要一个较小的导管，以实现妥?...

<table border="1"><tbody><tr><td> 试剂名称 </td><td> 公司 </td><td> 目录编号 </td></tr><tr><td>小动物聚焦超声系统</td><td>公司的FUS仪器， </td><td> RK-100 </td></tr><tr><td> DEFINITY </td><td> lantheus医学成像</td><td></td></tr><tr><td> OmniScan的</td><td> GE医疗集团</td><td></td></tr></tbody></table>

mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model

聚焦超声（FUS）破坏的血脑屏障（BBB）的是一个日益调查的技术绕过血脑屏障1-5。血脑屏障脑部疾病的药物治疗是一个重大的障碍，因为它限制从血管进入脑组织分子小于约500大小6大通过分子。引起血脑屏障破坏的FUS（BBBD）是暂时的和可逆的4对化学品的优势意味着，诱导高度本地化BBBD的。的FUS的诱导BBBD提供的治疗药物对大脑，组织足够的浓度，否则不会交付广泛的影响调查的一种手段。而广泛的超声参数已被证明成功的破坏血脑屏障2,5,7，在实验过程中有几个关键的步骤，以确保准确定位成功中断。这protocOL轮廓如何实现诱导的大鼠模型BBBD MRI引导下聚焦超音波，一个关键的动物实验的准备和气泡的处理步骤的重点。

Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

微泡介导的重点超声破坏血脑屏障（BBB）的是一个很有前途的技术，针对大脑中的非侵入性给药<sup&gt; 1-3</sup&gt;。该协议概述MRI引导下经颅血脑屏障破坏大鼠模型的实验过程。

MRI引导下的破坏血脑屏障在大鼠模型经颅聚焦超声

Focused ultrasound (FUS) disruption of the blood-brain barrier (BBB) is an increasingly investigated technique for circumventing the BBB1-5. The BBB is a ...

Research

JoVE Journal

Medicine

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

利用磁共振成像，容积电脑断层扫描及超音波的裸鼠乳腺癌骨转移大鼠模型血管生成的多模态成像

Angiogenesis is an essential feature of cancer growth and metastasis formation. In bone metastasis, angiogenic factors are pivotal for tumor cell ...

科研

医学

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

允许有针对性的大鼠模型血 - 脑屏障破坏神经血管治疗

Endothelial cells with tight junctions along with the basement membrane and astrocyte end feet surround cerebral blood vessels to form the blood-brain ...

A Model of Chronic Nutrient Infusion in the Rat

在大鼠模型慢性营养输液

Chronic exposure to excessive levels of nutrients is postulated to  affect the function of several organs and tissues and to contribute to the  ...

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

Stretch in Brain Microvascular Endothelial Cells cEND as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

作为脑微血管内皮细胞（CEND）的伸展<em&gt;体外</em创伤性脑损伤模型血脑屏障

Due to the high mortality incident brought about by traumatic brain injury (TBI), methods that would enable one to better understand the underlying ...

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Setting-up an In Vitro Model of Rat Blood-brain Barrier BBB: A Focus on BBB Impermeability and Receptor-mediated Transport

设置了一个<em&gt;体外</em大鼠血脑屏障&gt;模型（BBB）：在BBB抗渗性和受体介导的运输为中心

The blood brain barrier (BBB) specifically regulates molecular and cellular flux between the blood and the nervous tissue. Our aim was to develop and ...

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

测量升主动脉刚度<em&gt;在体内</em&gt;小鼠使用超声

We present a protocol for measuring in vivo aortic stiffness in mice using high-resolution ultrasound imaging. Aortic diameter is measured by ultrasound ...

Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction

Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction

缺氧缺糖再给氧作为<em&gt;在体外</em&gt;缺血再灌注损伤模型的研究血脑屏障功能障碍

Ischemia-Reperfusion (IR) injury is known to contribute significantly to the morbidity and mortality associated with ischemic strokes. Ischemic ...

MRI-guided dmPFC-rTMS as a Treatment for Treatment-resistant Major Depressive Disorder

MRI引导dmPFC-rTMS治疗作为治疗难治性抑郁症

Here we outline the protocol for magnetic resonance imaging (MRI) guided repetitive transcranial magnetic stimulation (rTMS) to the dorsal medial ...

Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model

直接节段性肝内交付使用大鼠肝门部夹具模型方法

Major hepatic surgery with inflow occlusion, and liver transplantation, necessitate a period of warm ischemia, and a period of reperfusion leading to ...

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

评估血脑屏障网状血管形成和破坏的体外测定

Blood-brain barrier (BBB) coverage plays a central role in the homeostasis of the central nervous system (CNS). The BBB is dynamically maintained by ...