Name: mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model
Uploaded: 2012-03-13T13:00:00
Description: Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

Forfatterne vil gerne takke Shawna Rideout-Gros og Alexandra Garcés for deres hjælp med pasning af dyr og Ping Wu for hende teknisk bistand. Støtten til dette arbejde blev givet af National Institutes of Health under ikke give. EB003268, og Canada Research Stole Program.

1. Ultralyd og MR Setup En MRI-kompatibel tre akser fokuseret ultralyd-systemet blev anvendt i denne undersøgelse (FUS Instruments, Inc., Toronto, Ontario, Canada). To forskellige ultralydstransducere blev anvendt: en intern konstrueret 551,5 kHz sfærisk fokuseret transducer (krumningsradius = 60 mm, udvendig diameter = 75 mm, indvendig diameter = 20 mm) og en 1,503 MHz, 8-sektor-array med integreret PZT hydrofon (Imasonic Inc., Voray-sur-L&#39;Orgnon, Frankrig), drevet som et enkelt element...

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Forberedelse af dyrene og mikrobobler er de mest kritiske aspekter af denne procedure. Pelsen på dyrets hoved skal helt fjernes for at undgå formildende ultralydbølgen. BBB kan forstyrres under isofluoran bedøvelse, men det bliver sværere at opnå ensartet åbning. Mikroboblerne bør altid håndteres med omhu og lille måler, skal store diameter nåle bruges ved udarbejdelse af, for at undgå at bryde dem. Tilsvarende bør den mindste gauge kateter, som med rimelighed kan anvendes i hal...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalog nummer </td></tr><tr><td> Mindre Husdyrs fokuseret ultralyd System </td><td> FUS Instruments, Inc. </td><td> RK-100 </td></tr><tr><td> Definity </td><td> Lantheus Medical Imaging </td><td></td></tr><tr><td> Omniscan </td><td> GE Healthcare </td><td></td></tr></tbody></table>

mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model

Fokuseret ultralyd (FUS) afbrydelse af blod-hjerne-barrieren (BBB) ​​er et stadig undersøges teknik til at omgå BBB 1-5. BBB er en væsentlig hindring for farmaceutiske behandlinger af hjernesygdomme, da den begrænser passagen af molekyler fra vaskulaturen i hjernevæv molekyler mindre end ca 500 Da i størrelse 6. FUS induceret BBB forstyrrelser (BBBD) er en midlertidig og reversibel 4 og har en fordel i forhold til kemiske midler for at inducere BBBD ved at være meget lokaliseret. FUS induceret BBBD tilvejebringer et middel til at undersøge virkningerne af en lang række terapeutiske midler på hjernen, der ellers ikke ville være leveres til vævet i tilstrækkelig koncentration. Mens en bred vifte af ultralyd parametre har vist sig gode til at forstyrre BBB 2,5,7, er der flere kritiske trin i den eksperimentelle procedure for at sikre en vellykket forstyrrelser med præcis målretning. Denne protokolol skitserer, hvordan man opnår MRI-styrede FUS inducerede BBBD i en rotte model, med fokus på den kritiske dyret forberedelse og mikroboblesystemer håndtering trin i eksperimentet.

Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

Mikroboble-medieret fokuseret ultralyd afbrydelse af blod-hjerne-barrieren (BBB) ​​er en lovende metode til ikke-invasiv målrettet lægemiddelafgivelse i hjernen<sup&gt; 1-3</sup&gt;. Denne protokol beskriver den eksperimentelle procedure for MR-guided transkraniel BBB forstyrrelse i en rotte model.

MR-guided Forstyrrelse af blod-hjerne barrieren ved hjælp af Transcranial fokuseret ultralyd i en rottemodel

Focused ultrasound (FUS) disruption of the blood-brain barrier (BBB) is an increasingly investigated technique for circumventing the BBB1-5. The BBB is a ...

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