Name: mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model
Uploaded: 2012-03-13T13:00:00
Description: Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

De auteurs willen graag Shawna Rideout-Gros en Alexandra Garces bedanken voor hun hulp bij de verzorging van dieren, en Ping Wu voor haar technische bijstand. Ondersteuning voor dit werk werd verstrekt door de National Institutes of Health in subsidie ​​niet. EB003268, en de Canada Research Chairs Program.

1. Echografie en MRI-instelling Een MRI-compatibele drie-assige focused ultrasound systeem werd gebruikt in dit onderzoek (FUS Instruments, Inc, Toronto, Ontario, Canada). Twee verschillende ultrasone transducenten werden gebruikt: een intern geconstrueerd 551,5 kHz bolvormige gerichte transducer (kromtestraal = 60 mm, een uitwendige diameter = 75 mm, een inwendige diameter 20 mm) en een 1,503 MHz, 8 sector array geïntegreerde PZT hydrofoon (Imasonic Inc, Voray-sur-L&#39;Orgnon, Frankrijk) ge...

<ol>
	<li>Kinoshita, M., McDannold, N., Jolesz, F. A., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+localized+delivery+of+Herceptin+to+the+mouse+brain+by+MRI-guided+focused+ultrasound-induced+blood-brain+barrier+disruption.">Noninvasive localized delivery of Herceptin to the mouse brain by MRI-guided focused ultrasound-induced blood-brain barrier disruption.</a> Proc. Natl. Acad. Sci. U.S.A. 103, 11719-11723 (2006).</li><li>Jord&#227;o, J. F., Ayala-Grosso, C. A., Markham, K., Huang, Y., Chopra, R., McLaurin, J., Hynynen, K., Aubert, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antibodies+targeted+to+the+brain+with+image-guided+focused+ultrasound+reduces+amyloid-beta+plaque+load+in+the+TgCRND8+mouse+model+of+Alzheimer&#39;s+disease.">Antibodies targeted to the brain with image-guided focused ultrasound reduces amyloid-beta plaque load in the TgCRND8 mouse model of Alzheimer&#39;s disease.</a> PLoS One. 5 (5), e10549-e10549 (2010).</li><li>Liu, H. -. L., Hua, M. -. Y., Chen, P. -. Y., Chu, P. -. C., Pan, C. -. H., Yang, H. -. W., Huang, C. -. Y., Wang, J. -. J., Yen, T. -. C., Wei, K. -. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood-brain+barrier+disruption+with+focused+ultrasound+enhances+delivery+of+chemotherapeutic+drugs+for+glioblastoma+treatment.">Blood-brain barrier disruption with focused ultrasound enhances delivery of chemotherapeutic drugs for glioblastoma treatment.</a> Radiology. 255, 415-425 (2010).</li><li>Hynynen, K., McDannold, N., Vykhodtseva, N., Jolesz, F. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+MR+imaging-guided+focal+opening+of+the+blood-brain+barrier+in+rabbits.">Noninvasive MR imaging-guided focal opening of the blood-brain barrier in rabbits.</a> Radiology. 220, 640-646 (2001).</li><li>Choi, J. J., Wang, S., Tung, Y. -. S., Morrison, B., Konofagou, E. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Molecules+of+various+pharmacologically-relevant+sizes+can+cross+the+ultrasound-induced+blood-brain+barrier+opening+in+vivo.">Molecules of various pharmacologically-relevant sizes can cross the ultrasound-induced blood-brain barrier opening in vivo.</a> Ultrasound Med. Biol. 36, 58-67 (2010).</li><li>Pardridge, W. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+blood-brain+barrier:+bottleneck+in+brain+drug+development.">The blood-brain barrier: bottleneck in brain drug development.</a> NeuroRx. 2, 3-14 (2005).</li><li>Bing, K. F., Howles, G. P., Qi, Y., Palmeri, M. L., Nightingale, K. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood-brain+barrier+&#40;BBB&#41;+disruption+using+a+diagnostic+ultrasound+scanner+and+Definity+in+mice.">Blood-brain barrier &#40;BBB&#41; disruption using a diagnostic ultrasound scanner and Definity in mice.</a> Ultrasound Med. Biol. 35, 1298-1308 (2009).</li><li>O'Reilly, M. A., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+PVDF+receiver+for+ultrasound+monitoring+of+transcranial+focused+ultrasound+therapy.">A PVDF receiver for ultrasound monitoring of transcranial focused ultrasound therapy.</a> IEEE Trans. Biomed. Eng. 57, 2286-2294 (2010).</li><li>Chopra, R., Curiel, L., Staruch, R., Morrison, L., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+MRI-compatible+system+for+focused+ultrasound+experiments+in+small+animal+models.">An MRI-compatible system for focused ultrasound experiments in small animal models.</a> Med. Phys. 36, 1867-1874 (2009).</li><li>McDannold, N., Zhang, Y., Vykhodtseva, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood-Brain+Barrier+Disruption+and+Vascular+Damage+Induced+by+Ultrasound+Bursts+Combined+with+Microbubbles+can+be+Influenced+by+Choice+of+Anesthesia+Protocol.">Blood-Brain Barrier Disruption and Vascular Damage Induced by Ultrasound Bursts Combined with Microbubbles can be Influenced by Choice of Anesthesia Protocol.</a> Ultrasound Med. Biol. , (2011).</li><li>McDannold, N., Vykhodtseva, N., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+disruption+of+the+blood-brain+barrier+with+focused+ultrasound:+association+with+cavitation+activity.">Targeted disruption of the blood-brain barrier with focused ultrasound: association with cavitation activity.</a> Phys. Med. Biol. 51, 793-807 (2006).</li><li>Yang, F. -. Y., Liu, S. -. H., Ho, F. -. M., Chang, C. -. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+ultrasound+contrast+agent+dose+on+the+duration+of+focused-ultrasound-induced+blood-brain+barrier+disruption.">Effect of ultrasound contrast agent dose on the duration of focused-ultrasound-induced blood-brain barrier disruption.</a> J. Acoust. Soc. Am. 126, 3344-3349 (2009).</li><li>McDannold, N., Vykhodtseva, N., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Blood-brain+barrier+disruption+induced+by+focused+ultrasound+and+circulating+preformed+microbubbles+appears+to+be+characterized+by+the+mechanical+index.">Blood-brain barrier disruption induced by focused ultrasound and circulating preformed microbubbles appears to be characterized by the mechanical index.</a> Ultrasound Med. Biol. 34, 834-840 (2008).</li><li>Neppiras, E. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acoustic+Cavitation.">Acoustic Cavitation.</a> Physics Reports (Review Section of Physics Letters). 61, 159-251 (1980).</li><li>O&#39;Reilly, M. A., Huang, Y., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+impact+of+standing+wave+effects+on+transcranial+focused+ultrasound+disruption+of+the+blood-brain+barrier+in+a+rat+model.">The impact of standing wave effects on transcranial focused ultrasound disruption of the blood-brain barrier in a rat model.</a> Phys. Med. Biol. 55, 5251-5267 (2010).</li></ol>

Voorbereiding van de dieren en microbellen zijn de meest kritische aspecten van deze procedure. Het haar op het hoofd van het dier moet in zijn geheel worden verwijderd om te voorkomen dat verzachtende het ultrasone geluid. De BBB kan worden verstoord onder isofluorane verdoving, echter, wordt het moeilijker om consistente opening. De microbolletjes altijd worden omgegaan en kleine overdruk moet grote diameter naalden worden bij het opstellen, om te voorkomen breken. Ook moet de kleinste gau...

<table border="1"><tbody><tr><td> Naam van het serum </td><td> Vennootschap </td><td> Catalogusnummer </td></tr><tr><td> Small Animal Focused Ultrasound System </td><td> FUS Instruments, Inc </td><td> RK-100 </td></tr><tr><td> Definity </td><td> Lantheus Medical Imaging </td><td></td></tr><tr><td> Omniscan </td><td> GE Healthcare </td><td></td></tr></tbody></table>

mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model

Focused Ultrasound (FUS) verstoring van de bloed-hersen barrière (BBB) ​​is een steeds onderzocht techniek voor het omzeilen van de BBB 1-5. De BBB is een belangrijke belemmering voor farmaceutische behandelingen van hersenaandoeningen zoals het beperkt de doorgang van moleculen uit de bloedvaten in het hersenweefsel om moleculen minder dan ongeveer 500 Da in maat 6. FUS geïnduceerde BBB verstoring (BBBD) is tijdelijk en omkeerbaar 4 en heeft een voordeel ten opzichte van chemische middelen voor het induceren van BBBD door zich zeer plaatselijk. FUS geïnduceerde BBBD een middel voor het onderzoeken van de effecten van een breed scala van therapeutische middelen op de hersenen, die anders niet zouden zijn leverbaar om het weefsel in voldoende concentratie. Hoewel een groot aantal ultrasone parameters bleken goed te verstoren de BBB 2,5,7, zijn er verschillende kritische stappen in de experimentele procedure voor succesvolle verstoring nauwkeurige targeting verzekeren. Dit protocolol wordt beschreven hoe u MRI-geleide FUS veroorzaakt BBBD in een ratmodel te bereiken, met een focus op de kritische dier voorbereiding en microbellen behandeling stappen van het experiment.

Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

Microbellen-gemedieerde focused ultrasound verstoring van de bloed-hersen barrière (BBB) ​​is een veelbelovende techniek voor niet-invasieve gerichte toediening van medicijnen in de hersenen<sup&gt; 1-3</sup&gt;. Dit protocol beschrijft de experimentele procedure voor MRI-geleide transcraniële BBB verstoring in een rat model.

MRI-geleide Verstoring van de bloed-hersen barrière met behulp van Transcraniële Focused Ultrasound in een ratmodel

Focused ultrasound (FUS) disruption of the blood-brain barrier (BBB) is an increasingly investigated technique for circumventing the BBB1-5. The BBB is a ...

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