Name: mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model
Uploaded: 2012-03-13T13:00:00
Description: Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

저자는 동물 보호와 그들의 도움을 쇼나 Rideout-Gros, 그리고 알렉 산드라의 Garces 감사드립니다, 그리고 그녀의 기술 지원 핑 우 것이다. 이 작품에 대한 지원 보조금없이 아래의 국립 보건원에 의해 제공되었다. EB003268, 그리고 캐나다 연구 의자 프로그램.

1. 초음파 및 MRI 설치 MRI 호환 세 축 집중 초음파 시스템은 (FUS 인 스트 루먼트 주식 회사, 토론토, 온타리오, 캐나다) 본 연구에서 사용되었다. 두 개의 서로 다른 초음파 트랜스 듀서가 사용되었습니다 통합과 내부 건설 551.5 kHz에서 이리저리 중심 변환기는 (곡률 반경 = 60mm, 외부 직경 = 75mm, 내경 = 20 ㎜)과 1.503 MHz의, 8 섹터 배열 PZT 수중 처음기 (Imasonic 주식 Voray-SUR-L&#39;Orgnon, 프...

<ol>
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동물과 microbubbles의 준비는이 절차의 가장 중요한 측면입니다. 동물의 머리에 머리를 완전히 초음파 빔을 attenuating 피하기 위해 제거해야합니다. BBB는 isofluorane 마취하에 중단될 수 있습니다 그러나, 그것은 일관된 개방을 달성하기 위해 더 어려워집니다. 그들을 위반하지 않도록하기 위해, 최대 그릴 때 microbubbles 항상 관심과 작은 게이지해서 다루어 져야, 큰 직경의 바늘을 ...

<table border="1"><tbody><tr><td> 시약의 이름 </td><td> 회사 </td><td> 카탈로그 번호 </td></tr><tr><td> 작은 동물들은 초음파 시스템을 중점 </td><td> FUS 인 스트 루먼트 주식 회사 </td><td> RK-100 </td></tr><tr><td> Definity </td><td> Lantheus 의료 이미징 </td><td></td></tr><tr><td> Omniscan </td><td> GE 헬스케어 </td><td></td></tr></tbody></table>

mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model

혈액 - 뇌 장벽 (BBB)의 초점 초음파 (FUS) 혼란은 BBB 1-5 우회 위해 점점 더 조사 기법입니다. 덜 크기가 6에서 약 500 다보다 분자로 뇌 조직에 vasculature에서 분자의 통과 제한으로 BBB는 뇌 장애의 제약 치료에 중요한 장애물이다. FUS 유도 BBB 장애 (BBBD)는 화학 물질 이상의 장점은 고도화된 됨으로써 BBBD 일으킬 의미 임시 및 4 가역하고 있습니다. FUS 유도 BBBD 달리 충분한 농도의 조직에 deliverable 않을 두뇌에 치료 요원들이 다양한 효과를 조사하는 수단을 제공합니다. 초음파 파라미터의 다양한 범위 BBB 2,5,7을 방해 성공적으로 증명했지만, 정확한 타겟팅과 성공적인 중단을 보장하기위한 실험 절차의 몇 가지 중요한 단계가 있습니다. 이 protoc좋았 윤곽선은 얼마나 중요한 동물의 준비와 실험의 microbubble 처리 단계를 중심으로 쥐 모델에서 BBBD 유도된 MRI-안내 FUS을 달성합니다.

Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

혈액 - 뇌 장벽 (BBB)​​의 Microbubble-매개 집중 초음파 장애는 두뇌의 비침습 타겟 약물 전달을위한 유망 기술입니다<sup&gt; 1-3</sup&gt;. 이 프로토콜은 쥐 모델에서 MRI를 유도 transcranial BBB 장애에 대한 실험 절차를 설명합니다.

쥐 모델에서 Transcranial 집중 초음파를 사용하여 혈액 - 뇌 장벽의 MRI를 유도 장애

Focused ultrasound (FUS) disruption of the blood-brain barrier (BBB) is an increasingly investigated technique for circumventing the BBB1-5. The BBB is a ...

Research

JoVE Journal

Medicine

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

자기 공명 영상, 체적 계산의 Tomography 및 초음파를 이용한 유방암의 뼈 전이의 누드 쥐 모델에서 Angiogenesis의 멀티 모달 이미징

Angiogenesis is an essential feature of cancer growth and metastasis formation. In bone metastasis, angiogenic factors are pivotal for tumor cell ...

연구

의학

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics

혈액 - 뇌 장벽 장애의 쥐 모델 Neurovascular 치료학 타겟이 분명한 허용하려면

Endothelial cells with tight junctions along with the basement membrane and astrocyte end feet surround cerebral blood vessels to form the blood-brain ...

A Model of Chronic Nutrient Infusion in the Rat

쥐의 만성 영양 주입의 모델

Chronic exposure to excessive levels of nutrients is postulated to  affect the function of several organs and tissues and to contribute to the  ...

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

Stretch in Brain Microvascular Endothelial Cells cEND as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

로 뇌 미세 혈관 내피 세포 (CEnd를)에 기지개<em&gt; 체외</em혈액 뇌 장벽의&gt; 외상성 뇌 손상 모델

Due to the high mortality incident brought about by traumatic brain injury (TBI), methods that would enable one to better understand the underlying ...

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Setting-up an In Vitro Model of Rat Blood-brain Barrier BBB: A Focus on BBB Impermeability and Receptor-mediated Transport

설정 - 업<em&gt; 체외</em쥐 뇌 혈관 벽의&gt; 모델 (BBB)​​ : BBB 불 침투성 및 수용체 매개 운송에 초점

The blood brain barrier (BBB) specifically regulates molecular and cellular flux between the blood and the nervous tissue. Our aim was to develop and ...

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

오름차순 대동맥 강성 측정<em&gt; 생체</em&gt; 초음파를 사용하여 마우스의

We present a protocol for measuring in vivo aortic stiffness in mice using high-resolution ultrasound imaging. Aortic diameter is measured by ultrasound ...

Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction

Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction

같은 산소 포도당 박탈과 재산 소화<em&gt; 체외</em&gt; 허혈 - 재관류 손상 모델 공부 뇌 혈관 장벽 부전

Ischemia-Reperfusion (IR) injury is known to contribute significantly to the morbidity and mortality associated with ischemic strokes. Ischemic ...

MRI-guided dmPFC-rTMS as a Treatment for Treatment-resistant Major Depressive Disorder

치료 저항성 주요 우울 장애에 대한 치료로 dmPFC-RTMS를 자기 공명 영상 유도

Here we outline the protocol for magnetic resonance imaging (MRI) guided repetitive transcranial magnetic stimulation (rTMS) to the dorsal medial ...

Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model

쥐 간 문부 클램프 모델을 사용하여 직접 분절 인트라 간 납품 방법

Major hepatic surgery with inflow occlusion, and liver transplantation, necessitate a period of warm ischemia, and a period of reperfusion leading to ...

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

혈액 뇌 장벽 메쉬와 유사한 혈관 형성 및 분열을 평가하기위한 In vitro 시험 법

Blood-brain barrier (BBB) coverage plays a central role in the homeostasis of the central nervous system (CNS). The BBB is dynamically maintained by ...