Name: mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model
Uploaded: 2012-03-13T13:00:00
Description: Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

Los autores desean agradecer a Shawna Rideout-Gros y Alexandra Garcés, por su ayuda con el cuidado de los animales, y Ping Wu por su asistencia técnica. El apoyo a este trabajo fue proporcionado por los Institutos Nacionales de Salud bajo la subvención núm. EB003268, y el Programa Canada Research Chairs.

1. La ecografía y la resonancia magnética de configuración Una resonancia magnética compatible con tres ejes del sistema de ultrasonido focalizado se utilizó en este estudio (FUS Instruments, Inc., Toronto, Ontario, Canadá). Dos transductores de ultrasonidos se utilizaron diferentes: uno en casa construida 551,5 kHz esféricamente centrada transductor (radio de curvatura = 60 mm, diámetro externo = 75 mm, diámetro interno = 20 mm), y un 1,503 MHz, 8-sector de matriz con integrado PZT h...

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Preparación de los animales y las microburbujas son los aspectos más críticos de este procedimiento. El pelo en la cabeza del animal debe ser totalmente eliminado para evitar la atenuación del haz de ultrasonidos. El BBB puede ser interrumpido bajo isofluorano anestésico, sin embargo, se hace más difícil de lograr apertura consistente. Las microburbujas siempre deben manipularse con cuidado y de pequeño calibre, agujas de gran diámetro debe ser utilizado en la elaboración, con el f...

<table border="1"><tbody><tr><td> Nombre del reactivo </td><td> Empresa </td><td> Número de catálogo </td></tr><tr><td> Pequeños Animales Sistema de Ultrasonido Enfocado </td><td> FUS Instruments, Inc. </td><td> RK-100 </td></tr><tr><td> Definity </td><td> Lantheus Medical Imaging </td><td></td></tr><tr><td> Omniscan </td><td> GE Healthcare </td><td></td></tr></tbody></table>

mri-guided disruption of the blood-brain barrier using transcranial focused ultrasound in a rat model

El ultrasonido focalizado (FUS) disrupción de la barrera hematoencefálica (BBB) ​​es una técnica cada vez más investigado por eludir el 1-5 BBB. La BHE es un obstáculo importante para los tratamientos farmacéuticos de los trastornos cerebrales, ya que limita el paso de moléculas de la vasculatura en el tejido cerebral de las moléculas de menos de aproximadamente 500 Da de tamaño 6. FUS inducida interrupción BBB (BBBD) es temporal y reversible 4 y tiene una ventaja sobre medios químicos de inducir BBBD por ser altamente localizada. FUS inducida BBBD proporciona un medio para investigar los efectos de una amplia gama de agentes terapéuticos en el cerebro, que de otro modo no sería entregable al tejido en concentración suficiente. Mientras que una amplia gama de parámetros de ultrasonido han demostrado su eficacia a perturbar el BBB 2,5,7, hay varios pasos críticos en el procedimiento experimental para asegurar la interrupción exitosa con precisión en el blanco. Este protoccontornos ol cómo lograr guiada por RM FUS BBBD inducidas en un modelo de rata, con un enfoque en la preparación de los animales y los pasos críticos de microburbujas de manejo del experimento.

Watch this Scientific Journal Video about MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model at JoVE.com

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model

Microburbujas mediada por la interrupción ultrasonido focalizado de la barrera hemato-encefálica (BHE) es una técnica prometedora para no invasiva la entrega de fármacos en el cerebro<sup&gt; 1-3</sup&gt;. Este protocolo describe el procedimiento experimental para la resonancia magnética transcraneal guiada por disrupción de la BHE en un modelo de rata.

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