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DOI :
10.3791/3562-v
January 17th, 2012
Chapters
0:05
Title
1:07
Small Scale Transformation of Bait Plasmid
2:12
Bait Validation
3:09
cDNA Library Screening
5:06
X-gal Assay
5:59
Retransformation in the MY2H Assay
6:47
Isolation of Progranulin-Interacting Proteins
7:32
Conclusion
我们已经修改了传统的酵母双杂交筛选,确定蛋白质相互作用的一种有效的遗传工具。此修改的过程明显缩短,减少了工作量,最重要的是,降低了误报数量。此外,这种方法是重复的,可靠的。
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