Name: large-scale recording of neurons by movable silicon probes in behaving rodents
Uploaded: 2012-03-04T14:00:00
Description: Watch this Scientific Journal Video about Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents at JoVE.com

Marie Curie International Outgoing Fellowship (Europese Unie FP/2007-2013 subsidieovereenkomsten # 221834 en 254780), JD McDonnell Foundation, NSF Grant SBE 0542013, National Institutes of Health Grant NS034994, National Institute of Mental Health Grant MH5467 en het Howard Hughes Medical Institute (Janelia Farm Research Campus subsidie).

1. De bouw van de microdrive Alle stations zijn van dezelfde basis-elementen: een bewegend deel, waarbij de elektrode en een vast deel, dat is verankerd aan de schedel draagt. Een ideale microdrive maakt glad, maar lang genoeg reizen van de elektrode in meerdere kleine stappen, is stevig genoeg om onbedoelde bewegingen van de elektrode, gemakkelijk te manipuleren door de experimentator zonder zich te bemoeien met het gedrag van het dier, klein van formaat en licht in gewicht te voorkomen. Als ...

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Deze film illustreert de implantatie procedure van silicium probes voor chronische grootschalige opnamen in de gedragen rat. Kritische stappen om de kwaliteit opnames van neuronale activiteit zorgen voor het gevolg zijn van de kwetsbaarheid van zowel biologische (hersenweefsel) en technische (silicium sonde) materialen. Speciale zorg dient te worden gehouden tijdens het hanteren van de sonde contact van schachten met afstand &quot;hard&quot; oppervlak (bijvoorbeeld de schachten zou breken wanneer men getracht te implant...

<table border="1"><tbody><tr><td> Naam </td><td> Type </td><td> Vennootschap </td><td> Catalogusnummer </td><td> Reacties </td></tr><tr><td> Silicon sonde Buzsaki32, 4 schachten x 8 sites. Verpakking: flexibele polyamide-kabel </td><td> Materiaal </td><td> NeuroNexus </td><td> Probe: buzsaki32 Verpakking: HC32 </td><td> Opname probe </td></tr><tr><td> Ronde messing schroef, 00-90 x 1/2 ronde messing schroeven </td><td> Materiaal </td><td> JIMorris </td><td> R0090B500 </td><td> Drive deel </td></tr><tr><td> Brass Hex Nut, 00-90 </td><td> Materiaal </td><td> JIMorris </td><td> N0090B </td><td> Drive deel </td></tr><tr><td> Brass C260 Strip, ASTM-B36 Dikte: 0,025 &quot;, Lengte: 12&quot;, breedte: 1/2 &quot; </td><td> Materiaal </td><td> Kleine onderdelen </td><td> B000FMYU72 </td><td> Drive deel </td></tr><tr><td> Connector Header, pitch 2 mm, male, enkele rij, kaarsrechte, 36 posities </td><td> Materiaal </td><td> Digikey </td><td> 2163S-36-ND </td><td> Drive deel </td></tr><tr><td> 2-delige Sylgard siliconen elastomeer </td><td> Materiaal </td><td> Wereld Precision Instruments </td><td> SYLG184 </td><td> Om extra-isoleren van de sonde </td></tr><tr><td> Decon Contrad 70 vloeibaar wasmiddel </td><td> Reagens </td><td> Fisher Scientific </td><td> 04-355 Decon Laboratories No: 1002 </td><td> Om de opname locaties schoon te maken </td></tr><tr><td> Impedantie Conditioning Module </td><td> Uitrusting </td><td> FHC Inc </td><td> 55-70-0 </td><td> Impedantie meter </td></tr><tr><td> niPOD - 32 kanalen </td><td> Uitrusting </td><td> Neuronexus </td><td> niPOD -32 </td><td> Impedantie meter </td></tr><tr><td> Grip Cement Industrial Grade </td><td> Materiaal </td><td> Caulk Dentsply </td><td> 675571 (poeder) 675572 (oplosmiddel) </td><td> Grip cement </td></tr><tr><td> 1,1 &#39;-dioctadecyl-3, 3,3&#39;, 3&#39;-tetramethylindocarbocyanine perchloraat (DII; DiIC18 (3)) </td><td> Reagens </td><td> Invitrogen </td><td> D282 </td><td> Om de sonde spoor vlek in de hersenen </td></tr><tr><td> RVS Machineschroef, Binding Head, Slotted Drive, # 00-90, 1/8 &quot; </td><td> Materiaal </td><td> Kleine onderdelen </td><td> MX-0090-02B </td><td> Grond-en referentie-schroeven </td></tr><tr><td> Magneetdraad, 20G, nylon-polyurethaan coating, MW80 </td><td> Materiaal </td><td> Kleine onderdelen </td><td> B000IJYRP2 </td><td> Grond-en referentie-draad </td></tr><tr><td> RVS Machineschroef, Binding hoofd Slotted Drive, # 000-120, 1/16 &quot; </td><td> Materiaal </td><td> Kleine onderdelen </td><td> MX-000120-01B </td><td> Anker schroeven </td></tr><tr><td> N-3 Alle doel Flux Liquid </td><td> Reagens </td><td> La-Co (Markal) </td><td> 23512 </td><td> Maakt het mogelijk om solderen van roestvrij staal </td></tr><tr><td> Microgrid Precision Uitgebreide Koper </td><td> Materiaal </td><td> Dexmet </td><td> 3 CU6-050 FA </td><td> Kopergaas on-head Faraday kooi </td></tr><tr><td> C &amp; B-METABOND Snel! Cement System - dentine Activator </td><td> Materiaal </td><td> Parkell </td><td> S380 </td><td></td></tr><tr><td> C &amp; B-METABOND Snel! Cement System - Dental cement </td><td> Materiaal </td><td> Parkell </td><td> S380 </td><td></td></tr><tr><td> Scherpe punt wolfraam naald en houder </td><td> Gereedschap </td><td> Roboz Chirurgische instrumenten </td><td> RS-6064 en RS-6061 </td><td> Om het haakje aan de dura te tillen </td></tr><tr><td> Carbide Bur HP 1/4 </td><td> Gereedschap </td><td> Henry Schein </td><td> 9990013 </td><td></td></tr><tr><td> Paraffine (granulaat) </td><td> Materiaal </td><td> Fisher Scientific </td><td> P31-500 </td><td></td></tr><tr><td> Minerale olie, Light (NF / FCC) </td><td> Materiaal </td><td> Fisher Scientific </td><td> O121-1 </td><td></td></tr><tr><td> GC ELECTRONICS 10-114 2-componenten epoxy lijm </td><td> Materiaal </td><td> Newark </td><td> 00Z416 </td><td></td></tr><tr><td> Type 1 Litz 21 AWG 40/36 Red Single polyurethaan-Nylon (MW80-c) om 0.041 &quot;+ / -0.002&quot; OD </td><td> Materiaal </td><td> New England Wire Technologies Corporation </td><td> N28-36E-400-2 </td><td> Om de kabel te maken tussen de headstage en de versterker </td></tr><tr><td> 32-kanaals Very Large Scale Integration headstage, 20x winst </td><td> Uitrusting </td><td> Plexon </td><td> HST/32V-G20 </td><td> Headstage </td></tr></tbody></table>

large-scale recording of neurons by movable silicon probes in behaving rodents

Een belangrijke uitdaging in de neurowetenschappen is het koppelen van het gedrag van de collectieve activiteiten van neurale assemblages. Begrip van de input-output relaties van neuronen en circuits vereist methoden met de ruimtelijke selectiviteit en temporele resolutie geschikt voor mechanistische analyse van de neurale ensembles in het gedrag van dieren, dat wil zeggen het opnemen van representatief grote monsters van enkele, geïsoleerde neuronen. Ensemble controle van de neuronale activiteit is opmerkelijk vooruitgang geboekt in het afgelopen decennium in zowel kleine als grote brein dieren, met inbegrip van menselijke proefpersonen 1-11. Meerdere plaatse opnemen met silicium-gebaseerde apparaten zijn vooral effectief vanwege hun schaalbaarheid, klein volume en geometrisch design. Hier beschrijven we methoden voor het opnemen van meerdere enkele neuronen en lokale veld potentieel in zich gedragen knaagdieren, met behulp van commercieel beschikbare micro-machinaal bewerkte silicium sensoren met op maat gemaakte accessoires componenten. Er zijn twee mogelijkheden fof bij verbinding silicium sondes naar voorversterkers: printplaten en flexibele kabels. Probe toeleveringsbedrijven ( <a href="http://www.neuronexustech.com/" >http://www.neuronexustech.com/</a> ; <a href="http://www.sbmicrosystems.com/">http://www.sbmicrosystems.com/</a> ; <a href="http://www.acreo.se/">http://www.acreo.se/</a> ) doorgaans de hechting service en leveren probes gebonden aan printplaten of flexibele kabels. Hier beschrijven we de implantatie van een 4-schacht, 32-site sonde verbonden aan flexibele polyimide-kabel, en gemonteerd op een beweegbare Microdrive. Elke stap van de probe preparaat wordt microdrive constructie en chirurgie geïllustreerd zodat de eindgebruiker gemakkelijk proces repliceren.

Watch this Scientific Journal Video about Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents at JoVE.com

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

We beschrijven methoden voor grootschalige opname van meerdere afzonderlijke eenheden en lokale veld potentieel in zich gedragen knaagdieren met siliconen sondes. Drive fabricage, sonde gehechtheid aan het station en de sonde implanteren worden geïllustreerd in voldoende details voor het gemakkelijk reproduceren.

Grootschalige Opname van Neuronen door Movable Silicon Probes in Behaving Knaagdieren

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of ...

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Neuroscience

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Opname Grootschalige Neuronale Ensembles met Silicon Probes in de verdoofde Rat

Large scale electrophysiological recordings from neuronal ensembles offer the opportunity to investigate how the brain orchestrates the wide variety of ...

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescerende Labeling Maakt Gerichte Extracellulair Single-unit Opnemen van geïdentificeerde neuronen<em&gt; In vivo</em

The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

Registratie en analyse van circadiane ritmen bij Running-wheel activiteit in knaagdieren

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

Opname Gamma Band oscillaties in Pedunculopontine Nucleus Neuronen

Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the ...

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Grootschalige Reconstructies en Onafhankelijk, Unbiased Clustering Gebaseerd op morfologische Metrics om neuronen te classificeren in Selective Populaties

This protocol outlines large-scale reconstructions of neurons combined with the use of independent and unbiased clustering analyses to create a ...

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Kwantificering van Endosome en Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

In the brain, membrane trafficking systems play important roles in regulating neuronal functions, such as neuronal morphology, synaptic plasticity, ...

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

Implantatie van chronische Silicon sondes en opname van Hippocampal plaats cellen in een verrijkte loopband-apparaat

An important requisite for understanding brain function is the identification of behavior and cell activity correlates. Silicon probes are advanced ...

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium beeldvorming van individuele neuronen in gedragen Caenorhabditis Elegans

It has become increasingly clear that neural circuit activity in behaving animals differs substantially from that seen in anesthetized or immobilized ...

Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

Bouw van een verbeterde multi Tetrode Hyperdrive voor grootschalige neurale opname in ratten gedragen

Monitoring the activity patterns of a large population of neurons over many days in awake animals is a valuable technique in the field of systems ...

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Preparaten en protocollen voor de hele cel Patch klem opname van Xenopus laevis Tectal neuronen

The Xenopus tadpole retinotectal circuit, comprised of the retinal ganglion cells (RGCs) in the eye which form synapses directly onto neurons in the optic ...