Name: large-scale recording of neurons by movable silicon probes in behaving rodents
Uploaded: 2012-03-04T14:00:00
Description: Watch this Scientific Journal Video about Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents at JoVE.com

Marie Curie International Outgoing Fellowship (EUs FP/2007-2013 tilskuddsavtalene # 221834 og 254780), JD McDonnell Foundation, NSF Grant 0542013 SBE, National Institutes of Health Grant NS034994, National Institute of Mental Health Grant MH5467 og Howard Hughes Medical Institute (Janelia Farm Forskning Campus stipend).

1. Byggingen av Microdrive Alle stasjoner er laget av de samme grunnleggende elementer: en bevegelig del, som bærer elektroden og en fast del, som er forankret til skallen. En ideell Microdrive lar glatt, men lenge nok reise av elektroden i flere små skritt, er solid nok til å hindre utilsiktet bevegelse av elektroden, lett å manipulere av eksperimentator uten å forstyrre dyrets oppførsel, liten i størrelse og lett i vekt. Som et resultat av disse konkurrerende krav, ulike stasjoner sui...

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Denne filmen illustrerer implantasjonsprosedyren av silisium prober for kroniske storskala opptak i oppføre rotte. Kritiske skritt for å sikre kvalitet opptak av neuronal aktivitet oppstår fra skjørheten i både biologisk (hjernevev) og tekniske (silisium probe) materialer. Spesiell forsiktighet bør utvises ved håndtering av sonden for å unngå enhver kontakt med skankene med noen eksternt &quot;hard&quot; overflate (for eksempel, ville Shanks brekke om man prøvde å implantere dem i hjernen uten å fjerne dura)...

<table border="1"><tbody><tr><td> Navn </td><td> Type </td><td> Firma </td><td> Catalog Number </td><td> Kommentarer </td></tr><tr><td> Silicon Buzsaki32 probe, 4 Shanks x 8 nettsteder. Emballasje: fleksibel polyamid kabel </td><td> Material </td><td> NeuroNexus </td><td> Probe: buzsaki32 Emballasje: HC32 </td><td> Opptak probe </td></tr><tr><td> Round Messingskrue, 00-90 x 1/2 Round messingskruer </td><td> Material </td><td> JIMorris </td><td> R0090B500 </td><td> Drive en del </td></tr><tr><td> Brass Sekskantmutter, 00-90 </td><td> Material </td><td> JIMorris </td><td> N0090B </td><td> Drive en del </td></tr><tr><td> Brass C260 Strip, ASTM-B36 Tykkelse: 0,025 &quot;, Lengde: 12&quot;, Bredde: 1/2 &quot; </td><td> Material </td><td> Små deler </td><td> B000FMYU72 </td><td> Drive en del </td></tr><tr><td> Connector Header, pitch 2mm, male, én rad, straigt, 36 stillinger </td><td> Material </td><td> Digikey </td><td> 2163S-36-ND </td><td> Drive en del </td></tr><tr><td> 2-delt Sylgard silisium Elastomer </td><td> Material </td><td> World presisjonsinstrumenter </td><td> SYLG184 </td><td> For ekstra isolere sonden </td></tr><tr><td> DECON Contrad 70 flytende vaskemiddel </td><td> Reagens </td><td> Fisher Scientific </td><td> 04-355 DECON Laboratories Nr.: 1002 </td><td> Slik rengjør innspillingen nettsteder </td></tr><tr><td> Impedans Conditioning Module </td><td> Utstyr </td><td> FHC Inc. </td><td> 55-70-0 </td><td> Impedans meter </td></tr><tr><td> niPOD - 32 kanaler </td><td> Utstyr </td><td> Neuronexus </td><td> niPOD -32 </td><td> Impedans meter </td></tr><tr><td> Grip Cement industriell karakter </td><td> Material </td><td> Caulk DENTSPLY </td><td> 675571 (pulver) 675572 (væske) </td><td> Grip cement </td></tr><tr><td> 1,1 &#39;-dioctadecyl-3, 3,3&#39;, 3&#39;-tetramethylindocarbocyanine perchlorate (&#39;Dii&#39;; DiIC18 (3)) </td><td> Reagens </td><td> Invitrogen </td><td> D282 </td><td> Å farge sonden spor i hjernen </td></tr><tr><td> Rustfritt stål Maskinskrue, binding Head, Slotted Drive, # 00-90, 1/8 &quot; </td><td> Material </td><td> Små deler </td><td> MX-0090-02B </td><td> Ground og referanse skruer </td></tr><tr><td> Magnet wire, 20G, nylon-polyurethane belegg, MW80 </td><td> Material </td><td> Små deler </td><td> B000IJYRP2 </td><td> Ground og referanse ledning </td></tr><tr><td> Rustfritt stål Maskinskrue, innbinding leder Slotted Drive, # 000-120, 1/16 &quot; </td><td> Material </td><td> Små deler </td><td> MX-000120-01B </td><td> Anchor skruer </td></tr><tr><td> N-3 All formål Flux Flytende </td><td> Reagens </td><td> La-Co (Markal) </td><td> 23512 </td><td> Tillater å lodde rustfritt stål </td></tr><tr><td> MicroGrid Precision Utvidet Copper </td><td> Material </td><td> Dexmet </td><td> 3 CU6-050 FA </td><td> Kobber mesh for on-head Faraday bur </td></tr><tr><td> C &amp; B-METABOND Raskt! Cement System - Dentin Activator </td><td> Material </td><td> Parkell </td><td> S380 </td><td></td></tr><tr><td> C &amp; B-METABOND Raskt! Cement System - Dental sement </td><td> Material </td><td> Parkell </td><td> S380 </td><td></td></tr><tr><td> Sharp poeng wolfram nål og tråd </td><td> Tool </td><td> Roboz Kirurgiske instrumenter </td><td> RS-6064 og RS-6061 </td><td> For å gjøre kroken til å løfte dura </td></tr><tr><td> Carbide Bur HP 1/4 </td><td> Tool </td><td> Henry Schein </td><td> 9990013 </td><td></td></tr><tr><td> Parafin (granulater) </td><td> Material </td><td> Fisher Scientific </td><td> P31-500 </td><td></td></tr><tr><td> Mineralolje, Lys (NF / FCC) </td><td> Material </td><td> Fisher Scientific </td><td> O121-1 </td><td></td></tr><tr><td> GC ELECTRONICS 10-114 2-Part epoksylim </td><td> Material </td><td> Newark </td><td> 00Z416 </td><td></td></tr><tr><td> Type 1 Litz 21 AWG 40/36 Rød Singel Polyuretan-Nylon (MW80-C) TO 0,041 &quot;+ / -0.002&quot; OD </td><td> Material </td><td> New England Wire Technologies Corporation </td><td> N28-36E-400-2 </td><td> For å gjøre kabelen mellom headstage og forsterkeren </td></tr><tr><td> 32-kanals Very Large Scale Integration headstage, 20x gevinst </td><td> Utstyr </td><td> Plexon </td><td> HST/32V-G20 </td><td> Headstage </td></tr></tbody></table>

large-scale recording of neurons by movable silicon probes in behaving rodents

En hovedutfordring i nevrovitenskap er knytte atferd til den kollektive aktiviteten nevrale forsamlinger. Forståelse av input-output relasjoner av nerveceller og kretser krever metoder med den romlige selektivitet og tidsmessig oppløsning hensiktsmessig for mekanistisk analyse av nevrale ensembler i oppfører dyret, dvs. opptak av representativ store prøver av isolerte single nerveceller. Ensemble overvåking av neuronal aktivitet har kommet bemerkelsesverdig i det siste tiåret i både små og store-brained dyr, inkludert mennesker 1-11. Flere stedet opptak med silisium-baserte enheter er spesielt effektive på grunn av sin skalerbarhet, lite volum og geometriske design. Her beskriver vi metoder for registrering flere single nevroner og lokale feltet potensial i å oppføre seg gnagere, bruke kommersielt tilgjengelige mikro-maskinerte silisium prober med skreddersydde individuelle behov. Det finnes to grunnleggende alternativer feller grensesnitt silisium sonder til preamplifiers: trykte kretskort og fleksible kabler. Probe forsyne bedrifter ( <a href="http://www.neuronexustech.com/" >http://www.neuronexustech.com/</a> , <a href="http://www.sbmicrosystems.com/">http://www.sbmicrosystems.com/</a> ; <a href="http://www.acreo.se/">http://www.acreo.se/</a> ) gir vanligvis den bonding service og levere prober bundet til kretskort eller fleksible kabler. Her beskriver vi implantasjon av en 4-shank, 32-site probe koblet til fleksibel polyimid kabel, og montert på en bevegelig Microdrive. Hvert trinn av sonden forberedelse, er Microdrive konstruksjon og operasjon illustrert slik at sluttbruker kan lett gjenskape prosessen.

Watch this Scientific Journal Video about Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents at JoVE.com

Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents

Vi beskriver metoder for storskala opptak av flere enkle enheter og lokale feltet potensial i å opptre gnagere med silisium sonder. Drive fabrikasjon, sonde tilknytning til stasjonen og probe implantasjon prosesser er illustrert i tilstrekkelige detaljer for enkel replikering.

Storskala opptak av nevroner med bevegelige Silicon Sonder i oppfører Gnagere

A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding of input-output relationships of ...

Research

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Neuroscience

Recording Large-scale Neuronal Ensembles with Silicon Probes in the Anesthetized Rat

Large scale electrophysiological recordings from neuronal ensembles offer the opportunity to investigate how the brain orchestrates the wide variety of ...

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

Retrograde Fluorescent Merking Tillater målrettet Ekstracellulær Single-enhet Opptak fra Identifiserte Nerveceller<em&gt; In vivo</em

The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from ...

Recording and Analysis of Circadian Rhythms in Running-wheel Activity in Rodents

When rodents have free access to a running wheel in their home cage, voluntary use of this wheel will depend on the time of day1-5. Nocturnal rodents, ...

Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

Opptak Gamma Band Svingninger i Pedunculopontine Nucleus Nerveceller

Synaptic efferents from the PPN are known to modulate the neuronal activity of several intralaminar thalamic regions (e.g., the ...

Large-scale Reconstructions and Independent, Unbiased Clustering Based on Morphological Metrics to Classify Neurons in Selective Populations

Storskala Rekonstruksjoner og Independent, Upartiske Clustering Basert på Morfologiske Metrics å klassifisere nevroner i Selektive populasjoner

This protocol outlines large-scale reconstructions of neurons combined with the use of independent and unbiased clustering analyses to create a ...

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Kvantifisering av endosom og lysosommotiliteter i dyrkede neuroner ved bruk av fluorescerende prober

In the brain, membrane trafficking systems play important roles in regulating neuronal functions, such as neuronal morphology, synaptic plasticity, ...

Implantation of Chronic Silicon Probes and Recording of Hippocampal Place Cells in an Enriched Treadmill Apparatus

Implantering av kronisk Silicon sonder og opptak av hippocampus sted celler i et beriket tredemølle apparat

An important requisite for understanding brain function is the identification of behavior and cell activity correlates. Silicon probes are advanced ...

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric kalsium avbilding av individuelle Neurons i oppfører seg Caenorhabditis Elegans

It has become increasingly clear that neural circuit activity in behaving animals differs substantially from that seen in anesthetized or immobilized ...

Construction of an Improved Multi-Tetrode Hyperdrive for Large-Scale Neural Recording in Behaving Rats

Byggingen av en forbedret flere sperrende Hyperdrive for store nevrale opptak i oppfører seg rotter

Monitoring the activity patterns of a large population of neurons over many days in awake animals is a valuable technique in the field of systems ...

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Forberedelser og protokoller for hele cellen Patch klemme opptak i Xenopus laevis Tectal nerveceller

The Xenopus tadpole retinotectal circuit, comprised of the retinal ganglion cells (RGCs) in the eye which form synapses directly onto neurons in the optic ...