Name: enhancement of apoptotic and autophagic induction by a novel synthetic c-1 analogue of 7-deoxypancratistatin in human breast adenocarcinoma and neuroblastoma cells with tamoxifen
Uploaded: 2012-05-30T12:00:00
Description: Watch this Scientific Journal Video about Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma

Questo lavoro è stato sostenuto dai Cavalieri di Colombo capitolo 9671 (Windsor, Ontario), e un CIHR Frederick Banting e Best Charles Canada Graduate Scholarship assegnato a Ma Dennis. Grazie a Hodge Robert ed Elizabeth Fidalgo da Silva per la loro assistenza con la microscopia time-lapse. Grazie a voi Katie Facecchia per la modifica dei time-lapse video microscopia. Vorremmo anche ringraziare Sudipa giugno Chatterjee e Phillip Tremblay per la revisione critica di questo manoscritto. Questo lavoro è dedicato in memoria di Kevin Couvillon che ha perso la sua battaglia contro il cancro nel 2010.

Introduzione L&#39;apoptosi, o di tipo I la morte cellulare programmata, è un processo fisiologico che può funzionare estrinsecamente, tramite legame di un ligando per un recettore morte, morte, o intrinsecamente. Il percorso intrinseco di apoptosi è iniziata da stress intracellulare, come il danno al DNA e disfunzione mitocondriale, questo conduce verso la permeabilizzazione dei mitocondri, dissipazione del potenziale di membrana mitocondriale (MMP), rilascio di fattori citotossici dallo sp...

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	<li>Earnshaw, W. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Apoptosis.+A+cellular+poison+cupboard.">Apoptosis. A cellular poison cupboard.</a> Nature. 397, 387-389 (1999).</li><li>Kroemer, G., Mari&#241;o, G., Levine, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Autophagy+and+the+integrated+stress+response.">Autophagy and the integrated stress response.</a> Mol. Cell. 40, 280-293 (2010).</li><li>Dalby, K. N., Tekedereli, I., Lopez-Berestein, G., Ozpolat, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeting+the+prodeath+and+prosurvival+functions+of+autophagy+as+novel+therapeutic+strategies+in+cancer.">Targeting the prodeath and prosurvival functions of autophagy as novel therapeutic strategies in cancer.</a> Autophagy. 6, 322-329 (2010).</li><li>Hanahan, D., Weinberg, R. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Hallmarks+of+cancer:+the+next+generation.">Hallmarks of cancer: the next generation.</a> Cell. 144, 646-674 (2011).</li><li>Fearon, E. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Human+Cancer+Syndromes:+Clues+to+the+Origin+and+Nature+of+Cancer.">Human Cancer Syndromes: Clues to the Origin and Nature of Cancer.</a> Science. 278, 1043-1050 (1997).</li><li>Tsokos, M., Scarpa, S., Ross, R. A., Triche, T. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differentiation+of+human+neuroblastoma+recapitulates+neural+crest+development.+Study+of+morphology,+neurotransmitter+enzymes,+and+extracellular+matrix+proteins.">Differentiation of human neuroblastoma recapitulates neural crest development. Study of morphology, neurotransmitter enzymes, and extracellular matrix proteins.</a> The American Journal of Pathology. 128, 484-496 (1987).</li><li>Schwab, M., Westermann, F., Hero, B., Berthold, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neuroblastoma:+biology+and+molecular+and+chromosomal+pathology.">Neuroblastoma: biology and molecular and chromosomal pathology.</a> Lancet Oncol. 4, 472-480 (2003).</li><li>Jemal, A., Siegel, R., Ward, E., Hao, Y., Xu, J., Thun, M. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cancer+statistics,+2009.">Cancer statistics, 2009.</a> CA Cancer J. Clin. 59, 225-249 (2009).</li><li>Howell, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+endocrine+prevention+of+breast+cancer.">The endocrine prevention of breast cancer.</a> Best Pract. Res. Clin. Endocrinol. Metab. 22, 615-623 (2008).</li><li>Moreira, P., Custodio, J., Morena, A., Oliveira, C., Santos, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tamoxifen+and+estradiol+interact+with+the+flavin+mononucleotide+site+of+complex+I+leading+to+mitochondrial+failure.">Tamoxifen and estradiol interact with the flavin mononucleotide site of complex I leading to mitochondrial failure.</a> J. Biol. Chem. 281, 10143-10152 (2006).</li><li>Kekre, N., Griffin, C., McNulty, J., Pandey, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancratistatin+causes+early+activation+of+caspase-3+and+the+flipping+of+phosphatidyl+serine+followed+by+rapid+apoptosis+specifically+in+human+lymphoma+cells.">Pancratistatin causes early activation of caspase-3 and the flipping of phosphatidyl serine followed by rapid apoptosis specifically in human lymphoma cells.</a> Cancer Chemother. Pharmacol. 56, 29-38 (2005).</li><li>McLachlan, A., Kekre, N., McNulty, J., Pandey, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancratistatin:+a+natural+anti-cancer+compound+that+targets+mitochondria+specifically+in+cancer+cells+to+induce+apoptosis.">Pancratistatin: a natural anti-cancer compound that targets mitochondria specifically in cancer cells to induce apoptosis.</a> Apoptosis. 10, 619-630 (2005).</li><li>Griffin, C., Hamm, C., McNulty, J., Pandey, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancratistatin+induces+apoptosis+in+clinical+leukemia+samples+with+minimal+effect+on+noncancerous+peripheral+blood+mononuclear+cells.">Pancratistatin induces apoptosis in clinical leukemia samples with minimal effect on noncancerous peripheral blood mononuclear cells.</a> Cancer Cell Int. 10, 6 (2010).</li><li>Griffin, C., Karnik, A., McNulty, J., Pandey, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancratistatin+selectively+targets+cancer+cell+mitochondria+and+reduces+growth+of+human+colon+tumor+xenografts.">Pancratistatin selectively targets cancer cell mitochondria and reduces growth of human colon tumor xenografts.</a> Molecular Cancer Therapeutics. 10, 57-68 (2011).</li><li>Griffin, C., McNulty, J., Pandey, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pancratistatin+induces+apoptosis+and+autophagy+in+metastatic+prostate+cancer+cells.">Pancratistatin induces apoptosis and autophagy in metastatic prostate cancer cells.</a> Int. J. Oncol. 38, 1549-1556 (2011).</li><li>Collins, J., Rinner, U., Moser, M., Hudlicky, T., Ghiviriga, I., Romero, A. E., Kornienko, A., Ma, D., Griffin, C., Pandey, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemoenzymatic+synthesis+of+Amaryllidaceae+constituents+and+biological+evaluation+of+their+C-1+analogues.+The+next+generation+synthesis+of+7-deoxypancratistatin+and+trans-dihydrolycoricidine.">Chemoenzymatic synthesis of Amaryllidaceae constituents and biological evaluation of their C-1 analogues. The next generation synthesis of 7-deoxypancratistatin and trans-dihydrolycoricidine.</a> J. Org. Chem. 75, 3069-3084 (2010).</li><li>Zhang, G., Gurtu, V., Kain, S. R., Yan, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+detection+of+apoptosis+using+a+fluorescent+conjugate+of+annexin+V.">Early detection of apoptosis using a fluorescent conjugate of annexin V.</a> Biotechniques. 23, 525-531 (1997).</li><li>Madesh, M., Hajn&#243;czky, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=VDAC-dependent+permeabilization+of+the+outer+mitochondrial+membrane+by+superoxide+induces+rapid+and+massive+cytochrome+c+release.">VDAC-dependent permeabilization of the outer mitochondrial membrane by superoxide induces rapid and massive cytochrome c release.</a> J. Cell Biol. 155, 1003-1015 (2001).</li><li>Simon, H. U., Haj-Yehia, A., Levi-Schaffer, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Role+of+reactive+oxygen+species+(ROS)+in+apoptosis+induction.">Role of reactive oxygen species (ROS) in apoptosis induction.</a> Apoptosis. 5, 415-418 (2000).</li><li>Batandier, C., Leverve, X., Fontaine, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Opening+of+the+mitochondrial+permeability+transition+pore+induces+reactive+oxygen+species+production+at+the+level+of+the+respiratory+chain+complex+I.">Opening of the mitochondrial permeability transition pore induces reactive oxygen species production at the level of the respiratory chain complex I.</a> J Biol Chem. 279, 17197-17204 (2004).</li><li>Lefranc, F., Sauvage, S., Goietsenoven, G. V. a. n., Mégalizzi, V., Lamoral-Theys, D., Debeir, O., Spiegl-Kreinecker, S., Berger, W., Mathieu, V., Decaestecker, C., Kiss, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Narciclasine,+a+plant+growth+modulator,+activates+Rho+and+stress+fibers+in+glioblastoma+cells.">Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells.</a> Mol. Cancer Ther. 8, 1739-1750 (2009).</li><li>Warburg, O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=On+the+origin+of+cancer+cells.">On the origin of cancer cells.</a> Science. 123, 309-314 (1956).</li><li>Heerdt, B. G., Houston, M. A., Augenlicht, L. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Growth+properties+of+colonic+tumor+cells+are+a+function+of+the+intrinsic+mitochondrial+membrane+potential.">Growth properties of colonic tumor cells are a function of the intrinsic mitochondrial membrane potential.</a> Cancer Res. 66, 1591-1596 (2006).</li><li>Green, D. R., Kroemer, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+pathophysiology+of+mitochondrial+cell+death.">The pathophysiology of mitochondrial cell death.</a> Science. 305, 626-629 (2004).</li><li>Casellas, P., Galiegue, S., Basile, A. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Peripheral+benzodiazepine+receptors+and+mitochondrial+function.">Peripheral benzodiazepine receptors and mitochondrial function.</a> Neurochem. Int. 40, 475-486 (2002).</li><li>Mathupala, S. P., Rempel, A., Pedersen, P. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Aberrant+glycolytic+metabolism+of+cancer+cells:+a+remarkable+coordination+of+genetic,+transcriptional,+post-translational,+and+mutational+events+that+lead+to+a+critical+role+for+type+II+hexokinase.">Aberrant glycolytic metabolism of cancer cells: a remarkable coordination of genetic, transcriptional, post-translational, and mutational events that lead to a critical role for type II hexokinase.</a> J. Bioenerg. Biomembr. 29, 339-343 (1997).</li></ol>

Composti PST e simili hanno dimostrato di avere proprietà anti-cancro 11-15,21. Abbiamo precedentemente riportato PST naturale di destabilizzare selettivamente i mitocondri nelle cellule tumorali, che in tal modo induce apoptosi mediante il rilascio di fattori citotossici 12,14. E &#39;molto probabile che JCTH-4 agisce attraverso lo stesso meccanismo; JCTH-4 ha causato il collasso MMP in MCF7 come si è visto con colorazione TMRM (Fig. 5a), e una maggiore generazione di ROS nei mi...

<table border="1"><tbody><tr><td> Nome del materiale </td><td> Azienda </td><td> Numero di catalogo </td></tr><tr><td> SH-SY5Y linea cellulare </td><td> ATCC </td><td> CRL-2266 </td></tr><tr><td> Dulbecco Modified Eagles Medio F-12 HAM </td><td> Sigma-Aldrich </td><td> 51448C </td></tr><tr><td> Siero fetale bovino </td><td> Gibco BRL </td><td> 16000-044 </td></tr><tr><td> MCF7 linea cellulare </td><td> ATCC </td><td> HTB-22 </td></tr><tr><td> Mezzo RPMI-1640 </td><td> Sigma-Aldrich </td><td> R 0883 </td></tr><tr><td> A quanto pare umano normale linea cellulare di fibroblasti fetali (NFF) </td><td> Coriell Institute for Medical Research </td><td> AG04431B </td></tr><tr><td> Media Dulbeccòs Modified Eagle, medio alto glucosio </td><td> Thermo Scientific </td><td> SH30022.01 </td></tr><tr><td> Tamoxifene sale </td><td> Sigma-Aldrich </td><td> T9262 </td&gt; </tr><tr><td> 35 mm vetro di fondo della cultura piatti </td><td> MatTek </td><td> P35G-014-C </td></tr><tr><td> Leica DMI6000 B microscopio invertito </td><td> Leica Microsystems </td><td> N / A </td></tr><tr><td> Colorante Hoechst 33342 </td><td> Molecular Probes </td><td> H3570 </td></tr><tr><td> Leica DM IRB invertito microscopio a fluorescenza </td><td> Leica Microsystems </td><td> N / A </td></tr><tr><td> Annessina V AlexaFluor-488 </td><td> Invitrogen </td><td> A13201 </td></tr><tr><td> Trypan Blu soluzione </td><td> Sigma-Aldrich </td><td> T8154-20ML </td></tr><tr><td> Emocitometro </td><td> Fisher Scientific </td><td> 267110 </td></tr><tr><td> WST-1 reagente </td><td> Roche Applied Science </td><td> 11644807001 </td></tr><tr><td> Wallac Victor 3 1420 Multilabel contatore </td><td> PerkinElmer </td><td> 1420-011 </td></tr><tr><td> Tetrametilrodamina metilestere (TMRM) </td><td> Gibco BRL </td><td> T-668 </td></tr><tr><td> Glass tessuto grinder </td><td> Fisher Scientific </td><td> K8885300-0002 </td></tr><tr><td> BioRad proteine ​​test </td><td> Bio-Rad Laboratories </td><td> 500 0001Bottom della Forma </td></tr><tr><td> Amplex Red </td><td> Invitrogen </td><td> A12222 </td></tr><tr><td> Perossidasi di rafano (HRP) </td><td> Sigma-Aldrich </td><td> P8125 </td></tr><tr><td> Spectramax Gemini XPS </td><td> Molecular Devices </td><td> 3126666 </td></tr><tr><td> Anti-LC3 anticorpo cresciuto in coniglio </td><td> Novus Biologicals </td><td> NB100-2220 </td></tr><tr><td> Anti-mouse HRP-coniugato anticorpo secondario </td><td> Abcam </td><td> ab6728 </td></tr><tr><td> Anti-coniglio HRP-anticorpo secondario coniugato </td><td> Abcam </td><td> ab6802 </td></tr><tr><td> Chemiluminescenza perossidasi substrato </td><td> Sigma-Aldrich </td><td> CPS160 </td></tr><tr><td> Monodansylcadaverine </td><td> Sigma-Aldrich </td><td> 30432 </td></tr></tbody></table>

enhancement of apoptotic and autophagic induction by a novel synthetic c-1 analogue of 7-deoxypancratistatin in human breast adenocarcinoma and neuroblastoma cells with tamoxifen

Il cancro al seno è uno dei tumori più diffusi tra le donne in Nord America. Molti attuali trattamenti anti-tumorali, incluse le radiazioni ionizzanti, inducono apoptosi attraverso danni al DNA. Sfortunatamente, tali trattamenti non sono selettivi per le cellule tumorali e produrre tossicità simile in cellule normali. Abbiamo riportato l&#39;induzione di apoptosi selettiva in cellule tumorali dal pancratistatin composto naturale (PST). Recentemente, un romanzo PST analogico, un derivato C-1 di 7-acetossimetil deoxypancratistatin (JCTH-4), è stato prodotto mediante sintesi de novo e presenta comparabili selettiva attività inducente l&#39;apoptosi nelle linee cellulari tumorali diverse. Recentemente, autofagia è stato implicato in neoplasie sia come pro-sopravvivenza e pro-morte meccanismi in risposta alla chemioterapia. Tamoxifene (TAM) ha sempre dimostrato l&#39;induzione di pro-sopravvivenza autofagia in numerosi tumori. In questo studio, l&#39;efficacia di JCTH-4 da solo e in combinazione con TAM per indurre la morte cellulare in significatività maternor (MCF7) e neuroblastoma (SH-SY5Y) le cellule è stata valutata. TAM solo indotto l&#39;autofagia, ma la morte delle cellule insignificante, mentre JCTH-4 da sola ha causato significativa induzione di apoptosi con alcuni induzione di autofagia. È interessante notare che il trattamento combinatoria ha prodotto un drastico aumento di induzione di apoptosi e autofagia. Abbiamo monitorato le variazioni morfologiche tempo-dipendenti MCF7 cellule in fase di TAM-indotta autofagia, JCTH-4-indotta l&#39;apoptosi e l&#39;autofagia e morte cellulare accelerata con trattamento combinatoria utilizzando microscopia time-lapse. Abbiamo dimostrato questi composti di indurre apoptosi / autofagia per spese mitocondriale in queste cellule tumorali. È importante sottolineare che questi trattamenti non ha influenzato la sopravvivenza di fibroblasti umani non tumorali. Così, questi risultati indicano che JCTH-4 in combinazione con TAM potrebbe essere usato come un sicuro e molto potente terapia antitumorale contro il cancro al seno e cellule di neuroblastoma.

Watch this Scientific Journal Video about Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma 

Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen

Abbiamo sintetizzato un analogo romanzo di pancratistatin paragonabile attività anti-cancro come pancratistatin nativo; interessante, il trattamento con tamoxifene combinatoria ha prodotto un miglioramento drastico induzione apoptotica e autophagic da mitocondriale di mira con effetti minimi sui fibroblasti non tumorali. Così, JCTH-4 in combinazione con tamoxifene potrebbe fornire una sicura terapia antitumorale.

Valorizzazione dei induzione apoptotica e autofagica da un romanzo sintetico C-1 analogico di 7-deoxypancratistatin in adenocarcinoma del seno umano e cellule di neuroblastoma con Tamoxifene

Breast cancer is one of the most common cancers amongst women in North America. Many current anti-cancer treatments, including ionizing radiation, induce ...

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