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DOI :
10.3791/3603-v
•
6:55 min
December 27th, 2011
Chapters
0:05
Title
1:28
Morpholino Preparation and Removing the Outer Corneal Layer
2:32
Corneal Incision and Morpholino Injection
3:31
Electroporation
4:55
Representative Morpholino Electroporation Results
6:14
Conclusion
有条件击倒在成年斑马鱼视网膜的目标蛋白质的表达,是一个方法,它涉及到玻璃体内注射反义morpholinos和电穿孔进入视网膜。由此产生的蛋白质是撞倒了好几天,这使得检测蛋白质的作用,在再生或完整的视网膜。
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