Name: magnetic resonance elastography methodology for the evaluation of tissue engineered construct growth
Uploaded: 2012-02-09T13:00:00
Description: Watch this Scientific Journal Video about Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth at JoVE.com

部分支持这项研究是由NIH RO3的EB007299-02和NSF EPSCoR第一奖。

1。组织构造的制备组织建设准备过程包括三个主要阶段：扩张的细胞群，细胞生物材料支架上播种，通过使用化学信号分子的分化。结构编写的程序是基于丹尼斯等 。，香港等 。，马里昂和毛泽东8,9,10进行的方法。 <ol><li>经过文化和扩张的细胞株，种子人类间质干细胞（H MSCS）到明胶海绵密度为1×10 6细胞/ ml（直径4毫米，厚度为3.5毫?...

<ol>
	<li>Xu, H., Othman, S. F., Magin, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+tissue+engineering+using+magnetic+resonance+imaging.">Monitoring tissue engineering using magnetic resonance imaging.</a> J. Biosci. Bioeng. 106, 515-527 (2008).</li><li>Xu, H., Othman, S. F., Hong, L., Peptan, I. A., Magin, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+resonance+microscopy+for+monitoring+osteogenesis+in+tissue-engineered+construct+in+vitro.">Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitro.</a> Phys. Med. Biol. 51, 719-732 (2006).</li><li>Othman, S. F., Xu, H., Royston, T. J., Magin, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microscopic+magnetic+resonance+elastography+(microMRE.">Microscopic magnetic resonance elastography (microMRE.</a> Magn. Reson. Med. 54, 605-615 (2005).</li><li>Muthupillai, R., Lomas, D. J., Rossman, P. J., Greenleaf, J. F., Manduca, A., Ehman, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+resonance+elastography+by+direct+visualization+of+propagating+acoustic+strain+waves.">Magnetic resonance elastography by direct visualization of propagating acoustic strain waves.</a> Science. 269, 1854-1857 (1995).</li><li>Othman, S. F., Curtis, E. T., Plautz, S. A., Pannier, A. P., Xu, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+resonance+elastography+monitoring+of+tissue+engineered+constructs.">Magnetic resonance elastography monitoring of tissue engineered constructs.</a> NMR Biomed. , (2011).</li><li>Oliphant, T. E., Manduca, A., Ehman, R. L., Greenleaf, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complex-valued+stiffness+reconstruction+for+magnetic+resonance+elastography+by+algebraic+inversion+of+the+differential+equation.">Complex-valued stiffness reconstruction for magnetic resonance elastography by algebraic inversion of the differential equation.</a> Magn. Reson. Med. 45, 299-310 (2001).</li><li>Ringleb, S. I., Chen, Q., Lake, D. S., Manduca, A., Ehman, R. L., An, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+shear+wave:+comparison+to+a+dynamic+shear+material+test.">Quantitative shear wave: comparison to a dynamic shear material test.</a> Magn. Reson. Med. 53, 1197-1201 (2005).</li><li>Hong, L., Peptan, I., Clark, P., Mao, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ex+vivo+adipose+tissue+engineering+by+human+marrow+stromal+cell+seeded+gelatin+sponge.">Ex vivo adipose tissue engineering by human marrow stromal cell seeded gelatin sponge.</a> Ann. Biomed. Eng. 33, 511-517 (2005).</li><li>Dennis, J. E., Haynesworth, S. E., Young, R. G., Caplan, A. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Osteogenesis+in+marrow-derived+mesenchymal+cell+porous+ceramic+composites+transplanted+subcutaneously:+effect+of+fibronectin+and+laminin+on+cell+retention+and+rate+of+osteogenic+expression.">Osteogenesis in marrow-derived mesenchymal cell porous ceramic composites transplanted subcutaneously: effect of fibronectin and laminin on cell retention and rate of osteogenic expression.</a> Cell Transplant. 1, 23-32 (1992).</li><li>Marion, N. W., Mao, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenchymal+stem+cells+and+tissue+engineering.">Mesenchymal stem cells and tissue engineering.</a> Methods Enzymol. 420, 339-361 (2006).</li><li>Rydberg, J., Grimm, R., Kruse, S., Felmlee, J., McCracken, P., Ehman, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+spin-echo+magnetic+resonance+elastography+of+the+brain.">Fast spin-echo magnetic resonance elastography of the brain.</a> , 1647-1647 (2001).</li><li>Kruse, S. A., Grim, R. C., Lake, D. S., Manduca, A., Ehman, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+EPI+based+3D+MR+elastography+of+the+brain.">Fast EPI based 3D MR elastography of the brain.</a> , 3385-3385 (2006).</li></ol>

在此过程中，为组织工程结构的麦格理的过程中表现出细胞制备一个elastogram的代。机械非破坏性评估方法应用组织工程管线，它现在可以评估多个发展阶段，整个工程结构变化。此外，麦格理补充监测组织工程建设，如扩散，磁化传递和化学位移分析1，其他议员的方法。 执行麦格理实验时，应注意一些限制。 体外标本的评估是一个敏感的时间研究。因此，建...

<table border="1"><tbody><tr><td> 材料名称 </td><td> 类型 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td> MSCGM的子弹包</td><td>试剂</td><td>龙沙</td><td> PT-3001 </td><td>保存于4°C间</td></tr><tr><td> 1X DPBS </td><td>试剂</td><td> Invitrogen公司</td><td> 21600-010 </td><td></td></tr><tr><td> 0.05％胰蛋白酶EDTA </td><td>试剂</td><td> Gibco公司，Invitrogen公司</td><td> 25300-054 </td><td>储存在-20°C </td></tr><tr><td>地塞米松</td><td>试剂</td><td> Sigma-Aldrich公司</td><td> D2915 </td><td></td></tr><tr><td> 3 - 异丁基-1 - 甲基黄嘌呤</td><td>试剂</td><td> Sigma-Aldrich公司</td><td> I5879 </td><td>储存在-20°C </td></tr><tr><td>胰岛素牛胰腺</td><td>试剂</td><td> Sigma-Aldrich公司</td><td> I6634 </td><td>储存在-20°C </td></tr><tr><td>吲哚美辛</td><td>试剂</td><td> Sigma-Aldrich公司</td><td> I7378 </td><td></td></tr><tr><td> β-甘油</td><td>试剂</td><td> Sigma-Aldrich公司</td><td> G9891 </td><td></td></tr><tr><td> L-抗坏血酸2 - 磷酸</td><td>试剂</td><td> Sigma-Aldrich公司</td><td> A8960 </td><td></td></tr><tr><td>明胶</td><td>脚手架</td><td>法玛西亚 - 普强公司</td><td> 09-0315-08 </td><td></td></tr><tr><td>人类间质干细胞</td><td>细胞株</td><td>龙沙</td><td> PT-2501 </td><td></td></tr><tr><td> 9.4T议员扫描器</td><td>设备</td><td>安捷伦</td><td></td><td> 400MHz的白平衡</td></tr><tr><td> 10毫米利兹线圈</td><td>设备</td><td>蒂科学</td><td></td><td></td></tr><tr><td>激光多普勒测振仪</td><td>设备</td><td>利达</td><td> PDV-100 </td><td></td></tr><tr><td> vibrosoft（20） </td><td>软件</td><td>利达</td><td></td><td></td></tr><tr><td>函数发生器</td><td>设备</td><td>安捷伦</td><td>的AFG 3022B </td><td></td></tr><tr><td>放大器</td><td>设备</td><td>压电陶瓷公司</td><td>环保局-104-115 </td><td></td></tr><tr><td>压电弯曲机</td><td>设备</td><td>压电INC。 </td><td> T234-A4Cl-203X </td><td></td></tr><tr><td>电脑Linux的</td><td>设备</td><td>处理器：英特尔酷睿2双核E8400 内存：2G </td><td></td><td></td></tr><tr><td>计算机Windows的</td><td>设备</td><td>处理器：英特尔酷睿2双核E8400 内存：2G </td><td></td><td></td></tr><tr><td> MATLAB的</td><td>软件</td><td> MathWorks公司</td><td></td><td> 2009B </td></tr></tbody></table>

magnetic resonance elastography methodology for the evaluation of tissue engineered construct growth

传统的机械测试结果往往破坏样品，并在长期组织工程建设研究的情况下，使用破坏性的评估是不能接受的。拟议的另一种方法是使用称为磁共振弹性成像过程。弹性是一种无损检测方法确定工程测量当地的机械属性值（即，复杂的剪切模量），这是确定一个组织的结构和功能的重要标志成果。作为一种非侵入性手段进行评价，监测工程结构，如磁共振成像（MRI）成像已经看到越来越多的兴趣在过去十年1。例如，磁共振（MR）扩散和relaxometry技术已经能够在化学和物理性质，化学性质的变化特点，在工程组织发展 。在提出的方法下面的协议，采用显微磁共振弹性（μMRE）的议员作为一种非侵入性测量小三软组织的力学性能的基础技术。麦格理是通过耦合声波的机械执行机构利益的组织，并记录与横波传播的磁共振扫描仪4。最近，μMRE已应用于组织工程，以获得增长的重要信息是传统的测量使用破坏性机械5宏观技术。在下面的过程中，弹性是通过与工程结构的改良加上机械执行机构哈恩自旋回波序列成像。如图1所示，修改后的顺序同步图像采集与传输的外部横波;随后，该议案是通过振荡双极对使用致敏。继图像采集与正面和负面的议案sensitizaTION，复杂的数据分工产生横波图像。然后，图像评估使用逆算法来生成剪切刚度地图6。在每个像素的测量结果已显示强烈相关（R&gt; 0.9914）收集的数据用动态力学分析7。在这项研究中，纳入监测人类间质干细胞（H，MSC）分化成脂肪细胞和骨结构如图2所示的组织发展过程中的弹性。

Watch this Scientific Journal Video about Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth at JoVE.com

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth

程序演示磁共振弹性的脂肪和骨组织，通过当地的机械性能，使用显微磁共振弹性（μMRE）的无创性评估的工程结构设计的结果监测方法。

磁共振弹性成像方法评价组织工程构造增长

Traditional mechanical testing often results in the destruction of the sample, and in the case of long term tissue engineered construct studies, the use ...

Research

JoVE Journal

Bioengineering

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue (EHT)

Bioluminescence Imaging for Assessment of Immune Responses Following Implantation of Engineered Heart Tissue EHT

生物发光成像评估的免疫反应，植入心脏组织工程（东隧）

Various techniques of cardiac tissue engineering have been pursued in the past decades including scaffolding strategies using either native or ...

科研

生物工程

Protocol for Relative Hydrodynamic Assessment of Tri-leaflet Polymer Valves

协议相对液力评估的三单张聚合物阀

Limitations of currently available prosthetic valves, xenografts, and homografts have prompted a recent resurgence of developments in the area of ...

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring

手术技术组织工程血管移植和后续的植入<em&gt;在体内</em&gt;监控

The development of Tissue Engineered Vessels (TEVs) is advanced by the ability to routinely and effectively implant TEVs (4-5 mm in diameter) into a large ...

Athymic Rat Model for Evaluation of Engineered Anterior Cruciate Ligament Grafts

无胸​​腺大鼠模型工程前交叉韧带移植评估

Anterior cruciate ligament (ACL) rupture is a common ligamentous injury that often requires surgery because the ACL does not heal well without ...

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

聚电解质复合物肝素结合域成骨生长因子交货

During reconstructive bone surgeries, supraphysiological amounts of growth factors are empirically loaded onto scaffolds to promote successful bone ...

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

人工程心脏组织的收缩自动分析心脏药物安全筛选

Cardiac tissue engineering describes techniques to constitute three dimensional force-generating engineered tissues. For the implementation of these ...

Metabolic Support of Excised, Living Brain Tissues During Magnetic Resonance Microscopy Acquisition

磁共振显微镜下切除活体脑组织的代谢支持

This protocol describes the procedures necessary to support normal metabolic functions of acute brain slice preparations during the collection of magnetic ...

Custom Engineered Tissue Culture Molds from Laser-etched Masters

由激光蚀刻大师定制的组织培养模具

As the field of tissue engineering has continued to mature, there has been increased interest in a wide range of tissue parameters, including tissue ...

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

原发性肾组织段的显微解剖与新型无支架结构技术的结合

Kidney transplantation is now a mainstream therapy for end-stage renal disease. However, with approximately 96,000 people on the waiting list and only ...

Manufacturing Abdominal Aorta Hydrogel Tissue-Mimicking Phantoms for Ultrasound Elastography Validation

超声弹性成像验证腹主动脉水凝胶组织模拟幻影的研制

Ultrasound (US) elastography, or elasticity imaging, is an adjunct imaging technique that utilizes sequential US images of soft tissues to measure the ...