Name: magnetic resonance elastography methodology for the evaluation of tissue engineered construct growth
Uploaded: 2012-02-09T13:00:00
Description: Watch this Scientific Journal Video about Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth at JoVE.com

इस शोध में भाग RO3-02-EB007299 NIH और NSF EPSCoR प्रथम पुरस्कार द्वारा समर्थित किया गया था.

1. ऊतक निर्माण तैयार ऊतक तैयार करने की प्रक्रिया के निर्माण के तीन मुख्य चरण होते हैं: सेल की आबादी का विस्तार, एक biomaterial पाड़ पर कोशिकाओं के बोने, रासायनिक संकेतन अणुओं के उपयोग के माध्यम से और भे...

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	<li>Xu, H., Othman, S. F., Magin, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+tissue+engineering+using+magnetic+resonance+imaging.">Monitoring tissue engineering using magnetic resonance imaging.</a> J. Biosci. Bioeng. 106, 515-527 (2008).</li><li>Xu, H., Othman, S. F., Hong, L., Peptan, I. A., Magin, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+resonance+microscopy+for+monitoring+osteogenesis+in+tissue-engineered+construct+in+vitro.">Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitro.</a> Phys. Med. Biol. 51, 719-732 (2006).</li><li>Othman, S. F., Xu, H., Royston, T. J., Magin, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microscopic+magnetic+resonance+elastography+(microMRE.">Microscopic magnetic resonance elastography (microMRE.</a> Magn. Reson. Med. 54, 605-615 (2005).</li><li>Muthupillai, R., Lomas, D. J., Rossman, P. J., Greenleaf, J. F., Manduca, A., Ehman, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+resonance+elastography+by+direct+visualization+of+propagating+acoustic+strain+waves.">Magnetic resonance elastography by direct visualization of propagating acoustic strain waves.</a> Science. 269, 1854-1857 (1995).</li><li>Othman, S. F., Curtis, E. T., Plautz, S. A., Pannier, A. P., Xu, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Magnetic+resonance+elastography+monitoring+of+tissue+engineered+constructs.">Magnetic resonance elastography monitoring of tissue engineered constructs.</a> NMR Biomed. , (2011).</li><li>Oliphant, T. E., Manduca, A., Ehman, R. L., Greenleaf, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Complex-valued+stiffness+reconstruction+for+magnetic+resonance+elastography+by+algebraic+inversion+of+the+differential+equation.">Complex-valued stiffness reconstruction for magnetic resonance elastography by algebraic inversion of the differential equation.</a> Magn. Reson. Med. 45, 299-310 (2001).</li><li>Ringleb, S. I., Chen, Q., Lake, D. S., Manduca, A., Ehman, R. L., An, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantitative+shear+wave:+comparison+to+a+dynamic+shear+material+test.">Quantitative shear wave: comparison to a dynamic shear material test.</a> Magn. Reson. Med. 53, 1197-1201 (2005).</li><li>Hong, L., Peptan, I., Clark, P., Mao, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ex+vivo+adipose+tissue+engineering+by+human+marrow+stromal+cell+seeded+gelatin+sponge.">Ex vivo adipose tissue engineering by human marrow stromal cell seeded gelatin sponge.</a> Ann. Biomed. Eng. 33, 511-517 (2005).</li><li>Dennis, J. E., Haynesworth, S. E., Young, R. G., Caplan, A. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Osteogenesis+in+marrow-derived+mesenchymal+cell+porous+ceramic+composites+transplanted+subcutaneously:+effect+of+fibronectin+and+laminin+on+cell+retention+and+rate+of+osteogenic+expression.">Osteogenesis in marrow-derived mesenchymal cell porous ceramic composites transplanted subcutaneously: effect of fibronectin and laminin on cell retention and rate of osteogenic expression.</a> Cell Transplant. 1, 23-32 (1992).</li><li>Marion, N. W., Mao, J. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenchymal+stem+cells+and+tissue+engineering.">Mesenchymal stem cells and tissue engineering.</a> Methods Enzymol. 420, 339-361 (2006).</li><li>Rydberg, J., Grimm, R., Kruse, S., Felmlee, J., McCracken, P., Ehman, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+spin-echo+magnetic+resonance+elastography+of+the+brain.">Fast spin-echo magnetic resonance elastography of the brain.</a> , 1647-1647 (2001).</li><li>Kruse, S. A., Grim, R. C., Lake, D. S., Manduca, A., Ehman, R. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fast+EPI+based+3D+MR+elastography+of+the+brain.">Fast EPI based 3D MR elastography of the brain.</a> , 3385-3385 (2006).</li></ol>

इस प्रक्रिया में, ऊतक इंजीनियर constructs के लिए MRE की प्रक्रिया सेल तैयारी से एक elastogram की पीढ़ी के लिए प्रदर्शन किया है. ऊतक इंजीनियरिंग पाइपलाइन के nondestructive यांत्रिक मूल्यांकन पद्धति लागू करके, यह अब संभव है है के...

<table border="1"><tbody><tr><td> सामग्री नाम </td><td> टाइप </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियाँ </td></tr><tr><td> MSCGM - बुलेट किट </td><td> अभिकर्मक </td><td> LONZA </td><td> PT-3001 </td><td> स्टोर 4 में डिग्री सेल्सियस </td></tr><tr><td> 1X DPBS </td><td> अभिकर्मक </td><td> Invitrogen </td><td> 21600-010 </td><td></td></tr><tr><td> 0.05% trypsin - EDTA </td><td> अभिकर्मक </td><td> Gibco, Invitrogen </td><td> 25300-054 </td><td> -20 डिग्री सेल्सियस पर स्टोर </td></tr><tr><td> Dexamethasone </td><td> अभिकर्मक </td><td> सिग्मा Aldrich </td><td> D2915 </td><td></td></tr><tr><td> 3 - Isobutyl-1-methylxanthine </td><td> अभिकर्मक </td><td> सिग्मा Aldrich </td><td> I5879 </td><td> -20 डिग्री सेल्सियस पर स्टोर </td></tr><tr><td> इंसुलिन गोजातीय अग्न्याशय </td><td> अभिकर्मक </td><td> सिग्मा Aldrich </td><td> I6634 </td><td> -20 डिग्री सेल्सियस पर स्टोर </td></tr><tr><td> Indomethacin </td><td> अभिकर्मक </td><td> सिग्मा Aldrich </td><td> I7378 </td><td></td></tr><tr><td> Β - Glycerophosphate </td><td> अभिकर्मक </td><td> सिग्मा Aldrich </td><td> G9891 </td><td></td></tr><tr><td> एल ascorbic 2 फॉस्फेट एसिड </td><td> अभिकर्मक </td><td> सिग्मा Aldrich </td><td> A8960 </td><td></td></tr><tr><td> Gelfoam </td><td> पाड़ </td><td> - फार्मेशिया और Upjohn कं </td><td> 09-0315-08 </td><td></td></tr><tr><td> मानव mesenchymal स्टेम सेल </td><td> सेल लाइन </td><td> LONZA </td><td> PT-2501 </td><td></td></tr><tr><td> 9.4T एमआर स्कैनर </td><td> उपकरण </td><td> Agilent है </td><td></td><td> 400MHz पश्चिम बंगाल </td></tr><tr><td> 10mm Litz कुंडल </td><td> उपकरण </td><td> सड़ा हुआ वैज्ञानिक </td><td></td><td></td></tr><tr><td> लेजर डॉपलर Vibrometer </td><td> उपकरण </td><td> Polytec </td><td> PDV-100 </td><td></td></tr><tr><td> Vibrosoft (20) </td><td> सॉफ्टवेयर </td><td> Polytec </td><td></td><td></td></tr><tr><td> फलन जनित्रकार्य </td><td> उपकरण </td><td> Agilent है </td><td> AFG 3022B </td><td></td></tr><tr><td> प्रवर्धक </td><td> उपकरण </td><td> Piezo कांग्रेस </td><td> EPA-104-115 </td><td></td></tr><tr><td> Piezo झुका मोटर </td><td> उपकरण </td><td> Piezo कांग्रेस. </td><td> T234 - A4Cl-203X </td><td></td></tr><tr><td> कंप्यूटर Linux </td><td> उपकरण </td><td> प्रोसेसर: इंटेल कोर 2 डुओ E8400 मेमोरी: 2 जी </td><td></td><td></td></tr><tr><td> कंप्यूटर विंडोज </td><td> उपकरण </td><td> प्रोसेसर: इंटेल कोर 2 डुओ E8400 मेमोरी: 2 जी </td><td></td><td></td></tr><tr><td> MATLAB </td><td> सॉफ्टवेयर </td><td> Mathworks, कांग्रेस </td><td></td><td> 2009b </td></tr></tbody></table>

magnetic resonance elastography methodology for the evaluation of tissue engineered construct growth

Traditional mechanical testing often results in the destruction of the sample, and in the case of long term tissue engineered construct studies, the use of destructive assessment is not acceptable. A proposed alternative is the use of an imaging process called magnetic resonance elastography. Elastography is a nondestructive method for determining the engineered outcome by measuring local mechanical property values (i.e., complex shear modulus), which are essential markers for identifying the structure and functionality of a tissue. As a noninvasive means for evaluation, the monitoring of engineered constructs with imaging modalities such as magnetic resonance imaging (MRI) has seen increasing interest in the past decade1. For example, the magnetic resonance (MR) techniques of diffusion and relaxometry have been able to characterize the changes in chemical and physical properties during engineered tissue development2. The method proposed in the following protocol uses microscopic magnetic resonance elastography (&mu;MRE) as a noninvasive MR based technique for measuring the mechanical properties of small soft tissues3. MRE is achieved by coupling a sonic mechanical actuator with the tissue of interest and recording the shear wave propagation with an MR scanner4. Recently, &mu;MRE has been applied in tissue engineering to acquire essential growth information that is traditionally measured using destructive mechanical macroscopic techniques5. In the following procedure, elastography is achieved through the imaging of engineered constructs with a modified Hahn spin-echo sequence coupled with a mechanical actuator. As shown in Figure 1, the modified sequence synchronizes image acquisition with the transmission of external shear waves; subsequently, the motion is sensitized through the use of oscillating bipolar pairs. Following collection of images with positive and negative motion sensitization, complex division of the data produce a shear wave image. Then, the image is assessed using an inversion algorithm to generate a shear stiffness map6. The resulting measurements at each voxel have been shown to strongly correlate (R2&gt;0.9914) with data collected using dynamic mechanical analysis7. In this study, elastography is integrated into the tissue development process for monitoring human mesenchymal stem cell (hMSC) differentiation into adipogenic and osteogenic constructs as shown in Figure 2.

Watch this Scientific Journal Video about Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth at JoVE.com

Magnetic Resonance Elastography Methodology for the Evaluation of Tissue Engineered Construct Growth

प्रक्रिया वसा और यांत्रिक सूक्ष्म चुंबकीय अनुनाद elastography (μMRE) का उपयोग गुण की noninvasive स्थानीय मूल्यांकन के माध्यम osteogenic ऊतक इंजीनियर constructs के इंजीनियर परिणाम की निगरानी के लिए चुंबकीय अनुनाद elastography की कार्यप्रणाली को दर्शाता है.

ऊतक के मूल्यांकन के लिए चुंबकीय अनुनाद Elastography पद्धति इंजीनियर निर्माण विकास

Traditional mechanical testing often results in the destruction of the sample, and in the case of long term tissue engineered construct studies, the use ...

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