Name: constructing a collagen hydrogel for the delivery of stem cell-loaded chitosan microspheres
Uploaded: 2012-06-01T12:00:00
Description: Watch this Scientific Journal Video about Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres at JoVE.com

DOZ Cenevre Vakfı tarafından verilen bir hibe ile desteklenmektedir. SN Pittsburgh Doku Mühendisliği Girişimi bir Doktora Sonrası Araştırma Bursu Grant tarafından desteklenmiştir.

1. Yalıtımlı Yağ-Türetilmiş Kök Hücre (ASC) Not: aksi belirtilmediği sürece tüm işlemler oda sıcaklığında yapıldı. <ol><li> Sıçan perirenal ve epididimal adipoz yalıtmak ve daha önce 6 açıklandığı gibi% 1 fetal sığır serumu (FBS) içeren steril Hank tamponlu tuz solüsyonu (HBSS) ile yıkayın. </li><li> Doku kıyma ve oda sıcaklığında 8 dakika boyunca 500 g&#39;de 50 mL tüpü ve santrifüj içine% 1 FBS içeren HBSS 25 mL içi...

<ol>
	<li>Krampera, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mesenchymal+stem+cells+for+bone,+cartilage,+tendon+and+skeletal+muscle+repair.">Mesenchymal stem cells for bone, cartilage, tendon and skeletal muscle repair.</a> Bone. 39, 678-683 (2006).</li><li>Patrick, C. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+engineering+strategies+for+adipose+tissue+repair.">Tissue engineering strategies for adipose tissue repair.</a> Anat. Rec. 263, 361-366 (2001).</li><li>Pountos, I., Giannoudis, P. V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biology+of+mesenchymal+stem+cells.">Biology of mesenchymal stem cells.</a> Injury. 36, S8-S12 (2005).</li><li>Kim, I. Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chitosan+and+its+derivatives+for+tissue+engineering+applications.">Chitosan and its derivatives for tissue engineering applications.</a> Biotechnol. Adv. 26, 1-21 (2008).</li><li>Shi, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+potential+of+chitosan+and+its+derivatives+in+regenerative+medicine.">Therapeutic potential of chitosan and its derivatives in regenerative medicine.</a> J. Surg. Res. 133, 185-192 (2006).</li><li>Natesan, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adipose-derived+stem+cell+delivery+into+collagen+gels+using+chitosan+microspheres.">Adipose-derived stem cell delivery into collagen gels using chitosan microspheres.</a> Tissue Eng. Part. A. 16, 1369-1384 (2010).</li><li>Bubnis, W. A., Ofner, C. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+determination+of+epsilon-amino+groups+in+soluble+and+poorly+soluble+proteinaceous+materials+by+a+spectrophotometric+method+using+trinitrobenzenesulfonic+acid.">The determination of epsilon-amino groups in soluble and poorly soluble proteinaceous materials by a spectrophotometric method using trinitrobenzenesulfonic acid.</a> Anal. Biochem. 207, 129-133 (1992).</li><li>Bornstein, M. 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Polym. 73, 201-211 (2008).</li><li>Haque, T., Chen, H., Ouyang, W., Martoni, C., Lawuyi, B., Urbanska, A., Prakash, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Investigation+of+a+new+microcapsule+membrane+combining+alginate,+chitosan,+polyethylene+glycol+and+poly-L-lysine+for+cell+transplantation+applications.">Investigation of a new microcapsule membrane combining alginate, chitosan, polyethylene glycol and poly-L-lysine for cell transplantation applications.</a> Int. J. Artif. Organs. 28, 631-637 (2005).</li><li>Goren, A., Dahan, N., Goren, E., Baruch, L., Machluf, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Encapsulated+human+mesenchymal+stem+cells:+a+unique+hypoimmunogenic+platform+for+long-term+cellular+therapy.">Encapsulated human mesenchymal stem cells: a unique hypoimmunogenic platform for long-term cellular therapy.</a> FASEB J. 24, 22-31 (2010).</li><li>Zielinski, B. A., Aebischer, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chitosan+as+a+matrix+for+mammalian+cell+encapsulation.">Chitosan as a matrix for mammalian cell encapsulation.</a> Biomaterials. 15, 1049-1056 (1994).</li><li>Girandon, L., Kregar-Velikonja, N., Bo&#382;ikov, K., Barli&#231;, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vitro+Models+for+Adipose+Tissue+Engineering+with+Adipose-Derived+Stem+Cells+Using+Different+Scaffolds+of+Natural+Origin.">In vitro Models for Adipose Tissue Engineering with Adipose-Derived Stem Cells Using Different Scaffolds of Natural Origin.</a> Folia Biol. (Praha). 57, 47-56 (2011).</li><li>Baruch, L., Machluf, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Alginate-chitosan+complex+coacervation+for+cell+encapsulation:+effect+on+mechanical+properties+and+on+long-term+viability.">Alginate-chitosan complex coacervation for cell encapsulation: effect on mechanical properties and on long-term viability.</a> Biopolymers. 82, 570-579 (2006).</li><li>Wei, Y., Gong, K., Zheng, Z., Wang, A., Ao, Q., Gong, Y., Zhang, X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chitosan/silk+fibroin-based+tissue-engineered+graft+seeded+with+adipose-derived+stem+cells+enhances+nerve+regeneration+in+a+rat+model.">Chitosan/silk fibroin-based tissue-engineered graft seeded with adipose-derived stem cells enhances nerve regeneration in a rat model.</a> J. Mater. Sci. Mater. Med. , (2011).</li><li>Wang, Q., Jamal, S., Detamore, M. S., Berkland, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=PLGA-chitosan/PLGA-alginate+nanoparticle+blends+as+biodegradable+colloidal+gels+for+seeding+human+umbilical+cord+mesenchymal+stem+cells.">PLGA-chitosan/PLGA-alginate nanoparticle blends as biodegradable colloidal gels for seeding human umbilical cord mesenchymal stem cells.</a> J. Biomed. Mater. Res. A. 96, 520-527 (2011).</li><li>Alves da Silva, M. L., Martins, A., Costa-Pinto, A. R., Correlo, V. M., Sol, P., Bhattacharya, M., Faria, S., Reis, R. L., Neves, N. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chondrogenic+differentiation+of+human+bone+marrow+mesenchymal+stem+cells+in+chitosan-based+scaffolds+using+a+flow-perfusion+bioreactor.">Chondrogenic differentiation of human bone marrow mesenchymal stem cells in chitosan-based scaffolds using a flow-perfusion bioreactor.</a> J. Tissue Eng. Regen. Med. , (2010).</li><li>Kang, Y. M., Lee, B. N., Ko, J. H., Kim, G. H., Kang, K. N., Kim da, Y., Kim, J. H., Park, Y. H., Chun, H. J., Kim, C. H., Kim, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+biocompatibility+study+of+electrospun+chitosan+microfiber+for+tissue+engineering.">In vivo biocompatibility study of electrospun chitosan microfiber for tissue engineering.</a> Int. J. Mol. Sci. 11, 4140-4148 (2010).</li><li>Bozkurt, G., Mothe, A. J., Zahir, T., Kim, H., Shoichet, M. S., Tator, C. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chitosan+channels+containing+spinal+cord-derived+stem/progenitor+cells+for+repair+of+subacute+spinal+cord+injury+in+the+rat.">Chitosan channels containing spinal cord-derived stem/progenitor cells for repair of subacute spinal cord injury in the rat.</a> Neurosurgery. 67, 1733-1744 (2010).</li><li>Leipzig, N. D., Wylie, R. G., Kim, H., Shoichet, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differentiation+of+neural+stem+cells+in+three-dimensional+growth+factor-immobilized+chitosan+hydrogel+scaffolds.">Differentiation of neural stem cells in three-dimensional growth factor-immobilized chitosan hydrogel scaffolds.</a> Biomaterials. 32, 57-64 (2011).</li><li>Altman, A. M., Gupta, V., RÃ­os, C. N., Alt, E. U., Mathur, A. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Adhesion,+migration+and+mechanics+of+human+adipose-tissue-derived+stem+cells+on+silk+fibroin-chitosan+matrix.">Adhesion, migration and mechanics of human adipose-tissue-derived stem cells on silk fibroin-chitosan matrix.</a> Acta Biomater. 6, 1388-1397 (2010).</li><li>Altman, A. M., Yan, Y., Matthias, N., Bai, X., Rios, C., Mathur, A. B., Song, Y. H., Alt, E. U. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=IFATS+collection:+Human+adipose-derived+stem+cells+seeded+on+a+silk+fibroin-chitosan+scaffold+enhance+wound+repair+in+a+murine+soft+tissue+injury+model.">IFATS collection: Human adipose-derived stem cells seeded on a silk fibroin-chitosan scaffold enhance wound repair in a murine soft tissue injury model.</a> Stem Cells. 27, 250-258 (2009).</li><li>Machado, C. B., Ventura, J. M., Lemos, A. F., Ferreira, J. M., Leite, M. F., Goes, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+chitosan-gelatin-chondroitin+porous+scaffold+improves+osteogenic+differentiation+of+mesenchymal+stem+cells.">3D chitosan-gelatin-chondroitin porous scaffold improves osteogenic differentiation of mesenchymal stem cells.</a> Biomed. Mater. 2, 124-131 (2007).</li></ol>

Kök hücre tabanlı tedavinin başlıca engellerden biri tamir için belirtilen bölgelere hücrelerin teslimat için etkin yöntemler geliştiriyor. Hastadan hastaya olan değişkenlik nedeniyle, doku tipi, yaralanma boyutu ve derinliği; teslim kök hücre yöntemi bir vaka ile ayrı ayrı tespit edilmelidir. Bir matris içinde kök hücreleri gömerek ve yara yerine onlara teslim doku mühendisliği için bir sonraki mantıklı bir yaklaşım gibi görünse de, bazı teknik engeller devam etmektedir. Bu matris yapı...

<table border="1" ><tbody><tr><td> Reaktif / ekipman Adı </td><td> Şirket </td><td> Katalog numarası </td><td> Yorumlar </td></tr><tr><td> Hanks BalancedSalt Çözüm (HBSS) </td><td> Gibco </td><td> 14175 </td><td> Sarf </td></tr><tr><td> Sığır fetüs serumu </td><td> Hyclone </td><td> SH30071.03 </td><td> Sarf </td></tr><tr><td> Kollajenaz Tip II </td><td> Sigma-Aldrich </td><td> C6685 </td><td> Sarf </td></tr><tr><td> 70 mikron naylon gözenekli filtre </td><td> BD Biosciences </td><td> 352350 </td><td> Sarf </td></tr><tr><td> 100 mikron naylon gözenekli filtre </td><td> BD Biosciences </td><td> 352360 </td><td> Sarf </td></tr><tr><td> MesenPRO Büyüme Orta Sistemi </td><td> Invitrogen </td><td> 12746-012 </td><td> Sarf </td></tr><tr><td> L-glutamin </td><td> Gibco </td><td> 25030 </td><td> Sarf </td></tr><tr><td> T75 Doku Kültürü Flask </td><td> BD Biosciences </td><td> 137787 </td><td> Sarf </td></tr><tr><td> Chitosan </td><td> Sigma-Aldrich </td><td> 448869 </td><td> Sarf </td></tr><tr><td> Asetik asit </td><td> Sigma-Aldrich </td><td> 320099 </td><td> Sarf </td></tr><tr><td> N-oktanol </td><td> Acros Organics </td><td> 150630025 </td><td> Sarf </td></tr><tr><td> Sorbitan-Mono-oleat </td><td> Sigma-Aldrich </td><td> S6760 </td><td> Sarf </td></tr><tr><td> Potasyum hidroksit </td><td> Sigma-Aldrich </td><td> P1767 </td><td> Sarf </td></tr><tr><td> Aseton </td><td> Fisher Scientific </td><td> L-4859 </td><td> Sarf </td></tr><tr><td> Etanol </td><td> Sigma-Aldrich </td><td> 270741 </td><td> Sarf </td></tr><tr><td> Trinitro benzensülfonik asit </td><td> Sigma-Aldrich </td><td> P2297 </td><td> Sarf </td></tr><tr><td> Hidroklorik asit </td><td> Sigma-Aldrich </td><td> 320331 </td><td> Sarf </td></tr><tr><td> Etil eter </td><td> Sigma-Aldrich </td><td> 472-484 </td><td> Sarf </td></tr><tr><td> 8-um Doku Kültürü Plaka Uçlar </td><td> BD Biosciences </td><td> 353097 </td><td> Sarf </td></tr><tr><td> 1.5 ml Mikrosantrifüj Tüpler </td><td> Balıkçı </td><td> 05-408-129 </td><td> Sarf </td></tr><tr><td> MTT Reaktif </td><td> Invitrogen </td><td> M6494 </td><td> Sarf </td></tr><tr><td> Dimetil Sulfoxide </td><td> Sigma-Aldrich </td><td> D8779 </td><td> Sarf </td></tr><tr><td> Qtracker Hücre Etiketleme Kiti (Q tracker 655) </td><td> Molecular Probes </td><td> Q2502PMP </td><td> Sarf </td></tr><tr><td> 1 Kollajen Tip </td><td> Travigen </td><td> 3447-020-01 </td><td> Sarf </td></tr><tr><td> Sodyum Hidroksit </td><td> Sigma-Aldrich </td><td> S8045 </td><td> Sarf </td></tr><tr><td> 12-Peki Doku Kültürü Tabaklar </td><td> BD Biosciences </td><td> 353043 </td><td> Sarf </td></tr><tr><td> Santrifüj </td><td> Eppendorf </td><td> 5417R </td><td> Ekipman </td></tr><tr><td> Orbital Çalkalayıcı </td><td> New Brunswick Bilimsel </td><td> C24 </td><td> Ekipman </td&gt; </tr><tr><td> Hava-% 5 CO2 ile nemlendirilmiş Kuluçka </td><td> Thermo Scientific </td><td> Model 370 </td><td> Ekipman </td></tr><tr><td> Tepegöz Karıştırıcı </td><td> IKA </td><td> Visc6000 </td><td> Ekipman </td></tr><tr><td> Manyetik Karıştırıcı </td><td> Corning </td><td> PC-210 </td><td> Ekipman </td></tr><tr><td> Vakum Desikatör </td><td> - </td><td> - </td><td> Ekipman </td></tr><tr><td> Parçacık Boyut Analiz Cihazı </td><td> Malvern </td><td> STP2000 Spraytec </td><td> Ekipman </td></tr><tr><td> Su Banyosu </td><td> Fisher Scientific </td><td> Isotemp210 </td><td> Ekipman </td&gt; </tr><tr><td> Spektrofotometre </td><td> Beckman </td><td> Beckman Coulter DU800UV/Visible Spektrofotometre </td><td> Ekipman </td></tr><tr><td> Girdap </td><td> Diagger </td><td> 3030a </td><td> Ekipman </td></tr><tr><td> Mikroplaka Okuyucu </td><td> Molecular Devices </td><td> SpectraMax M2 </td><td> Ekipman </td></tr><tr><td> Floresan / Işık Mikroskobu </td><td> Olympus </td><td> IX71 </td><td> Ekipman </td></tr><tr><td> Konfokal Mikroskop </td><td> Olympus </td><td> Konfokal Mikroskop FV-500 Lazer </td><td> Ekipman </td></tr><tr><td> Taramalı Elektron Mikroskobu </td><td> Carl Zeiss MicroImaging </td><td> Leo 435 VP </td><td> Ekipman </td></tr><tr><td> Transmisyon Elektron Mikroskobu </td><td> JEOL </td><td> JEOL 1230 </td><td> Ekipman </td></tr></tbody></table>

constructing a collagen hydrogel for the delivery of stem cell-loaded chitosan microspheres

Multipotent kök hücreleri rejeneratif tıp 1-3 alanında çok faydalı olduğu kanıtlanmıştır. Bununla birlikte, doku rejenerasyonu için etkili bir şekilde, bu hücrelerin kullanmak için, değişken bir dizi dikkate alınmalıdır. Bu değişkenler şunlardır: toplam hacmi ve implantasyon sitenin yüzey alanı, doku mekanik özellikleri ve damarlanma miktarını ve hücre dışı matris bileşenlerini kapsamaktadır doku mikro,. Ana doku taklit eden bir mekanik mukavemet korurken Bu nedenle, bu hücrelerin sağlamak için kullanılan materyallerin belirlenmiş bir kimyasal bileşimi ile biyo-uyumlu olmalıdır. Bu malzemeler ayrıca oksijen ve besin hücrelere tutunur ve çoğalırlar için elverişli bir mikro sağlamak için geçirgen olması gerekir. Chitosan, mükemmel biyouyumluluk bir katyonik polisakkarit, kolayca kimyasal olarak değiştirilmiş ve in vivo mac in ile bağlamak için yüksek afinite edilebilirromolecules 4-5. Chitosan, hücre yapışması, göçü ve çoğalması için bir substrat olarak işlev sağlayan, ekstraselüler matriksin glikozaminoglikan kısmı taklit eder. Bu çalışmada, üç boyutlu bir yapı iskelesi 6 göre kollajen bir içine adipoz-türevi kök hücreleri (Artan) sunmak üzere mikrosferler şeklinde kitosan kullanmaktadır. İdeal bir hücreden mikrosfer oranı inkübasyon süresi ve yüklenmesi olabilir hücrelerin sayısını elde etmek için hücre yoğunluğu açısından değerlendirildi. ASC kitosan mikroküreler (CSM) üzerine ekildikten sonra, bir kollajen iskele gömülü olan ve uzun süre kültüründe muhafaza edilebilir. Özetle, bu çalışmada hassas bir üç boyutlu yapı iskelesi biyomalzemenin içinde kök hücreleri sunmak için bir yöntem sağlar.

Watch this Scientific Journal Video about Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres at JoVE.com

Constructing a Collagen Hydrogel for the Delivery of Stem Cell-loaded Chitosan Microspheres

Mevcut kök hücre tedavilerinde büyük bir engel konak dokularına bu hücrelerin sunmak için en etkili yöntem belirlemektir. Adipoz kaynaklı kök hücreler kendi multipotency korumasını sağlarken Burada, verimli ve yaklaşım, basit bir kitosan tabanlı dağıtım yöntemi açıklar.

Kök Hücre yüklü Chitosan Mikrokürelerin teslimi için bir Kollajen Hidrojel oluşturma

Multipotent stem cells have been shown to be extremely useful in the field of regenerative medicine1-3. However, in order to use these cells effectively ...

Research

JoVE Journal

Bioengineering

Organotypic Collagen I Assay: A Malleable Platform to Assess Cell Behaviour in a 3-Dimensional Context

Organotipik Kollajen Ben Assay: 3-Boyutlu Bağlamında Hücre Davranışı değerlendirin A Dövülebilir Platformu

Cell migration is fundamental to many aspects of biology, including development, wound healing, the cellular responses of the immune system, and ...

Engineering a Bilayered Hydrogel to Control ASC Differentiation

ASC farklılaşma kontrol için bir iki tabakalı Hidrojel mühendislik

Natural polymers over the years have gained more importance because of their host biocompatibility and ability to interact with cells in vitro and in ...

An Improved Method for the Preparation of Type I Collagen From Skin

Cilt Tip I Kolajen hazırlanması için geliştirilmiş bir yöntem

Soluble type 1 collagen (COL1) is used extensively as an adhesive substrate for cell cultures and as a cellular scaffold for regenerative applications. ...

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Doğallığı Kollajen Tellerinin Görüntüleme<em&gt; In vivo</em&gt; Ve<em&gt; Ex vivo</em&gt; Kafesli Kollajen Mimetik Peptidlerinin Fotoğraf-tetiklenen Hybridization yoluyla

Collagen is a major structural component of the extracellular matrix that supports tissue formation and maintenance. Although collagen remodeling is an ...

Synthesis of an Intein-mediated Artificial Protein Hydrogel

Bir Intein aracılı yapay protein Hidrojel sentezi

We present the synthesis of a highly stable protein hydrogel mediated by a split-intein-catalyzed protein trans-splicing reaction. The building blocks of ...

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Için Amelogenin-kitosan hidrojelin geliştirilmesi<em&gt; In Vitro</emBir Yoğun Arayüzü ile&gt; Emaye büyütme

Biomimetic enamel reconstruction is a significant topic in material science and dentistry as a novel approach for the treatment of dental caries or ...

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

Microscale Vortex destekli Sıralı Moleküler Teslimat için Electroporator

Electroporation has received increasing attention in the past years, because it is a very powerful technique for physically introducing non-permeant ...

An Injectable and Drug-loaded Supramolecular Hydrogel for Local Catheter Injection into the Pig Heart

Domuz Kalbe Yerel Kateter Enjeksiyon için bir Enjekte ve İlaç yüklü Supramoleküler Hidrojel

Regeneration of lost myocardium is an important goal for future therapies because of the increasing occurrence of chronic ischemic heart failure and the ...

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Rekombinant kolajen peptid Microcarriers hücre genişletme ve hücre teslim sistemi bir biyoreaktör olarak potansiyel kullanım için ben Model

Tissue engineering is a promising field, focused on developing solutions for the increasing demand on tissues and organs regarding transplantation ...

Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel

Bir böbrek korteks ekstraselüler matrisi elde edilen hidrojel imalatı

Extracellular matrix (ECM) provides important biophysical and biochemical cues to maintain tissue homeostasis. Current synthetic hydrogels offer robust ...