Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

Cette étude a été soutenue par la bourse R01 et R21 Nihm MH091816 MH084043 au TMD.

1. Matériels et Méthodes 1.1. Les réservoirs d&#39;eau Les réservoirs cylindriques (30 cm de hauteur x 20 cm diamètre) requis pour le test de natation de souris forcée (FST) dans notre laboratoire sont construits en plexiglas transparent, ce matériau est capable de résister aux mouvements fréquents des réservoirs et des accidents de mieux que le verre. Le niveau d&#39;eau est de 15 cm du fond et doit être marqué sur le réservoir pour s&#39;assurer que le ...

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La TSF (parfois appelé test de natation Porsolt) a été développé d&#39;abord pour les rats et les souris modifiées pour en Porsolt et collègues 12,13. En plus du protocole ci-dessus décrites avec succès dans notre laboratoire, un certain nombre de modifications de test largement subtiles ont été publiés (voir Hascoët et Bourin pour un examen complet 1). C&#39;est un test couramment utilisé pour l&#39;évaluation de l&#39;efficacité des médicaments anti-dépresseurs et les effets des...

<ul><li> Les réservoirs d&#39;eau </li><li> Thermomètre </li><li> Minuterie </li><li> Caméra Vidéo </li><li> Générateur de bruit blanc (facultatif) </li><li> Papier de séchage </li></ul>

the mouse forced swim test

Le test de la nage forcée est un test de comportement chez les rongeurs utilisés pour l&#39;évaluation des médicaments antidépresseurs, l&#39;efficacité antidépressive de nouveaux composés, et des manipulations expérimentales qui visent à rendre ou à prévenir la dépression ressemblant à des États. Les souris sont placées dans un réservoir incontournable transparente qui est rempli avec de l&#39;eau et leur comportement de mobilité liés échapper est mesurée. Le test de la nage forcée est simple à effectuer de façon fiable et qu&#39;il requiert un minimum d&#39;équipement spécialisé. Mise en œuvre réussie de l&#39;épreuve de natation forcée exige l&#39;adhésion à certains détails de procédure et la minimisation des contraintes injustifiées à la souris. Dans la description du protocole et de la vidéo qui l&#39;accompagne, nous expliquons comment mener la version de ce test de la souris avec un accent sur les pièges potentiels qui peuvent être préjudiciables à l&#39;interprétation des résultats et comment les éviter. En outre, nous expliquer comment les comportements se manifeste dans l&#39;épreuve sont évalués.

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

Le test de la nage forcée est validée comme une approche expérimentale pour évaluer le potentiel d&#39;efficacité des antidépresseurs chez les rongeurs. Les animaux de laboratoire sont placés dans une cuve d&#39;eau et d&#39;évasion liés à des comportements de mobilité est quantifié. Les procédures communes pour la version la souris de ce test sont décrits.

Le test de natation forcée souris

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

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