Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

Questo studio è stato supportato dal NIHM concedere MH091816 R01 e R21 MH084043 di TDG.

1. Materiali e metodi 1.1. Il serbatoi d&#39;acqua I serbatoi cilindrici (30 cm di altezza x 20 diametro cm) necessari per il test del nuoto forzato (FST) nel nostro laboratorio sono realizzati in plexiglas trasparente, in quanto questo materiale è in grado di resistere alle frequenti spostamenti dei carri armati e gli incidenti meglio di vetro. Il livello dell&#39;acqua è di 15 cm dal fondo e deve essere segnato sul serbatoio per assicurare che il volume di acqua ?...

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La FST (a volte chiamato test nuotare Porsolt) è stato sviluppato prima per i ratti e poi modificato per topi da Porsolt e colleghi 12,13. In aggiunta a quanto sopra-descritto il protocollo di successo nel nostro laboratorio, una serie di modifiche largamente prova sottili sono stati pubblicati (vedi Hascoet e Bourin per una revisione completa 1). Si tratta di un test comunemente utilizzato per la valutazione dell&#39;efficacia di farmaci anti-depressivi e gli effetti di varie manipolazioni compor...

<ul><li> Serbatoi d&#39;acqua </li><li> Termometro </li><li> Timer </li><li> Videocamera </li><li> Generatore di rumore bianco (opzionale) </li><li> Asciugatura della carta </li></ul>

the mouse forced swim test

Il test di nuoto forzato è un test comportamentale roditori utilizzati per la valutazione dei farmaci antidepressivi, efficacia antidepressiva di nuovi composti, e le manipolazioni sperimentali che mirano a rendere o prevenire gli stati depressivi simile. I topi sono posti in una vasca trasparente inevitabile che si riempie con l&#39;acqua e il loro comportamento mobilità fuga relativo è misurato. Il test di nuoto forzato è semplice da effettuare in modo affidabile e che richiede minime attrezzature specializzate. Il successo del test di nuoto forzato richiede l&#39;osservanza di determinati dettagli procedurali e ridurre al minimo lo stress ingiustificato per i topi. Nella descrizione del protocollo e il video di accompagnamento, spieghiamo come condurre la versione del mouse di questo test con particolare attenzione ai trabocchetti potenziali che possono essere pregiudizievoli per l&#39;interpretazione dei risultati e come evitarli. Inoltre, abbiamo spiegato come i comportamenti manifestati nel test sono valutati.

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

Il test di nuoto forzato è convalidato come un approccio sperimentale per valutare il potenziale efficacia antidepressiva nei roditori. Animali da laboratorio sono collocati in una vasca di acqua e fuga comportamento relativo alla mobilità è quantificato. Le procedure comuni per la versione del mouse di questa prova sono descritti.

La forzata mouse test Swim

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

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