Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

本研究では、助成金NIHM R01 MH091816とTDGのR21 MH084043によってサポートされています。

1。材料と方法 1.1。水タンクこの材料はガラスよりも優れて戦車や事故の頻繁な動きに耐えることができるようになると我々の研究室でマウスの強制水泳試験（FST）に必要な円筒形のタンクは（30 cm高さ× 20 ​​cmの直径）、透明なプレキシグラスで構成されています。水位は底から15 cmであり、水の量は、マウス全体で整合性を確保するようにタンクに?...

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FST（と呼ばれることもありますPorsoltの水泳試験）は、ラットで最初に開発し、Porsoltと同僚12,13によってマウスのために変更されました。我々の研究室で成功し、上記のプロトコルに加えて、主に微妙なテストの修正の数は、（完全な見直し1 HascoëtとBourinを参照してください）発表されています。それは抗うつ薬と基礎および臨床研究3,14-16で様々な行動と神経生物学...

<ul><li>水タンク</li><li>温度計</li><li>タイマー</li><li>ビデオカメラ</li><li>ホワイトノイズ発生器（オプション） </li><li>乾燥紙</li></ul>

the mouse forced swim test

強制水泳試験は抗うつ薬、新規化合物の抗うつ効果、および状態のようなうつ病をレンダリングまたは予防を目的としている実験操作の評価に使用されるげっ歯類の行動試験です。マウスは、水で満たされており、彼らの脱出に関連するモビリティの挙動が測定され避けられない透明なタンクに配置されます。強制水泳試験では、確実に実施するのは簡単であり、それは最小限の特殊な装置を必要とします。強制水泳試験の実装を成功させるには、一定の手続きの詳細およびマウスへの不当なストレスの最小化に従う必要があります。プロトコルの説明および添付のビデオでは、我々はどのようにそれらを避けるために、結果の解釈とに有害かもしれない潜在的な落とし穴に重点を置いてこの試験のマウスのバージョンを実施する方法について説明します。さらに、我々はテストで明らかに動作が評価される方法について説明します。

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

強制水泳試験は、げっ歯類の潜在的な抗うつ効果を評価するための実験的なアプローチとして検証されます。実験動物は、水のタンクに配置され、エスケープ関連のモビリティの挙動が定量化される。この試験のマウスのバージョンに共通の手順が説明されています。

マウス強制水泳試験

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

Research

JoVE Journal

Neuroscience

One Dimensional Turing-Like Handshake Test for Motor Intelligence

モーター知能一次元チューリングライクハンドシェイクテスト

In the Turing test, a computer model is deemed to "think intelligently" if it can generate answers that are not distinguishable from those of a human.  ...

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

複数のマウスの神経解剖学的磁気共鳴イメージング

The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, motivated by the need for improved tools for characterizing and ...

Detection of Neuritic Plaques in Alzheimer's Disease Mouse Model

アルツハイマー病のマウスモデルにおける神経突起斑の検出

Alzheimer's disease (AD) is the most common neurodegenerative disorder leading to dementia. Neuritic plaque formation is one of the pathological hallmarks ...

T-maze Forced Alternation and Left-right Discrimination Tasks for Assessing Working and Reference Memory in Mice

マウスで作業していて、メモリの参照を評価するためのT-迷路強制交替と左右弁別課題

Forced alternation and left-right discrimination tasks using the T-maze have been widely used to assess working and reference memory, respectively, in ...

Tissue Preparation and Immunostaining of Mouse Sensory Nerve Fibers Innervating Skin and Limb Bones

スキンと四肢骨の神経支配マウス感覚神経線維の組織の調製と免疫染色

Detection and primary processing of physical, chemical and thermal sensory stimuli by peripheral sensory nerve fibers is key to sensory perception in ...

The Tail Suspension Test

尾懸垂テスト

The tail-suspension test is a mouse behavioral test useful in the screening of potential antidepressant drugs, and assessing of other manipulations that ...

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

マウス海馬にオリゴマーアミロイドベータの定位注入

Alzheimer&#8217;s disease is a neurodegenerative disease affecting the aging population. A key neuropathological feature of the disease is the ...

The Fibular Nerve Injury Method: A Reliable Assay to Identify and Test Factors That Repair Neuromuscular Junctions

腓骨神経損傷方法：神経筋接合部を修復信頼性の高い同定するためのアッセイおよびテスト要因

The neuromuscular junction (NMJ) undergoes deleterious structural and functional changes as a result of aging, injury and disease. Thus, it is imperative ...

Automated Analysis of C. elegans Swim Behavior Using CeleST Software

Automated Analysis of C. elegans Swim Behavior Using CeleST Software

の自動解析<em&gt; C。エレガンス</emセレストソフトウェアを使用&gt;スイム挙動

Dissecting the neuronal and neuromuscular circuits that regulate behavior remains a major challenge in biology. The nematode Caenorhabditis elegans has ...

Diffuse Reflectance Spectroscopy: Getting the Capillary Refill Test Under One's Thumb

拡散反射率分光: 取得 1 つの親指の下の毛細血管リフィル テスト

The capillary refill test was introduced in 1947 to help estimate circulatory status in critically ill patients. Guidelines commonly state that refill ...