Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

Denne studien har vært støttet av tilskuddet NihM R01 MH091816 og R21 MH084043 til TDG.

1. Materiale og metode 1.1. Den vanntanker Den sylindriske tanker (30 cm høyde x 20 cm diameter) som kreves for musen tvunget svømme test (FST) i vårt laboratorium er laget av gjennomsiktig pleksiglass, da dette materialet er i stand til å tåle hyppige bevegelser av tankene og ulykker bedre enn glass. Vannstanden er 15 cm fra bunnen og skal være merket på tanken for å sikre at volumet av vann er konsistente på tvers av mus. Antallet tankene bør ideelt sett v...

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Den FST (noen ganger kalt Porsolt svømme test) ble utviklet først for rotter og deretter endret for mus ved Porsolt og kolleger 12,13. I tillegg til de ovenfor beskrevet protokoll vellykket i vårt laboratorium, har en rekke hovedsakelig subtile test modifikasjoner er publisert (se Hascoët og Bourin for en fullstendig gjennomgang 1). Det er en vanlig test som brukes for evaluering av effekten av anti-depressive medikamenter og effekten av ulike atferdsmessige og nevrobiologiske manipulasjoner i ...

<ul><li> Vanntanker </li><li> Termometer </li><li> Timer </li><li> Videokamera </li><li> White Noise Generator (valgfritt) </li><li> Tørking Paper </li></ul>

the mouse forced swim test

Den tvang svømme testen er en gnager atferdsdata test brukt for vurdering av antidepressive legemidler, antidepressive effekten av nye forbindelser, og eksperimentelle manipulasjoner som er rettet mot gjengivelse eller forebygge depressive-lignende tilstander. Mus er plassert i en uunngåelig gjennomsiktig tank som er fylt med vann og deres flukt relatert mobilitet atferd er målt. Den tvang svømme testen er enkelt å foreta en pålitelig og det krever minimalt med spesialisert utstyr. Vellykket gjennomføring av tvang svømme testen krever tilslutning til visse prosessuelle detaljer og minimalisering av uberettiget stress til musene. I protokollen beskrivelse og tilhørende video, forklarer vi hvordan du utfører musen versjon av denne testen med vekt på potensielle fallgruver som kan være skadelig for tolkning av resultater og hvordan du kan unngå dem. I tillegg forklarer vi hvordan atferd manifestert i testen er vurdert.

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

Den tvang svømme testen er validert som en eksperimentell tilnærming for å vurdere potensiell antidepressiv effekt hos gnagere. Forsøksdyr er plassert i en tank med vann og rømme-relaterte mobilitet adferd er kvantifisert. Den vanlige prosedyrer for musen versjon av denne testen er beskrevet.

The Mouse Tvunget Swim Test

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

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