Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

Este estudo foi apoiado pelo NIHM conceder R01 e R21 MH091816 MH084043 para TDG.

1. Materiais e Método 1.1. Os tanques de água Os tanques cilíndricos (30 cm de altura x 20 cm de diâmetro), necessários para o teste de natação forçada do mouse (FST) em nosso laboratório são feitas de acrílico transparente, como este material é capaz de suportar o movimento freqüente dos tanques e acidentes melhor do que o vidro. O nível da água é de 15 cm do fundo e devem ser marcadas no tanque para garantir que o volume de água é consistente em ca...

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O FST (às vezes chamado de teste de natação Porsolt) foi desenvolvido em primeiro lugar para os ratos e depois modificado para camundongos por Porsolt e colegas 12,13. Além do protocolo acima descrito sucesso em nosso laboratório, uma série de modificações em grande parte testar sutis têm sido publicados (ver Hascoët e Bourin para uma revisão completa 1). É um teste comum usado para a avaliação da eficácia de anti-depressivos e os efeitos das várias manipulações comportamentais e ...

<ul><li> Tanques de água </li><li> Termômetro </li><li> Cronômetro </li><li> Filmadora </li><li> Gerador de ruído branco (opcional) </li><li> Secagem de papel </li></ul>

the mouse forced swim test

O teste de natação forçada é um teste comportamental de roedores utilizados para a avaliação de medicamentos antidepressivos, eficácia antidepressiva de novos compostos, e manipulações experimentais que visam a prestação ou impedindo-depressiva como estados. Os ratos são colocados em um tanque transparente inescapável que é preenchido com água e sua fuga comportamento de mobilidade relacionados é medido. O teste de natação forçada é simples de realizar de forma confiável e requer equipamento especializado mínimo. Implementação bem sucedida do teste de natação forçada exige a adesão a certos detalhes processuais e de minimização do estresse injustificado para os ratos. Na descrição do protocolo e que acompanha o vídeo, explicamos como conduzir a versão do mouse deste teste, com ênfase em armadilhas potenciais que podem ser prejudiciais à interpretação dos resultados e como evitá-los. Além disso, explicamos como os comportamentos manifestados no teste são avaliados.

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

O teste de natação forçada é validado como uma abordagem experimental para avaliar a eficácia do antidepressivo potencial em roedores. Animais experimentais são colocados em um tanque de água e fugir de comportamentos relacionados à mobilidade é quantificado. Os procedimentos comuns para a versão do mouse deste teste são descritos.

O Mouse Teste de Natação Forçada

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

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