Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

Este estudio ha sido financiado por la beca R01 y R21 NiHM MH091816 MH084043 a TDG.

1. Material y Método 1.1. Los tanques de agua Los tanques cilíndricos (30 cm de altura x 20 cm diámetro) requerido para la prueba de natación forzada del ratón (FST) en nuestro laboratorio se construyen de plexiglás transparente, ya que este material es capaz de soportar los frecuentes movimientos de los tanques y los accidentes mejor que el vidrio. El nivel del agua es de 15 cm del fondo y debe ser marcada en el tanque para asegurarse de que el volumen de agua ...

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La FST (a veces llamada prueba de natación Porsolt) fue desarrollado por primera vez para las ratas y ratones modificados para Porsolt y sus colegas 12,13. Además de los protocolos descritos anteriormente con éxito en nuestro laboratorio, un gran número de modificaciones sutiles pruebas se han publicado (ver Hascoët y Bourin para una revisión completa 1). Se trata de una prueba común para la evaluación de la eficacia de los fármacos antidepresivos y los efectos de varias manipulaciones de ...

<ul><li> Tanques de agua </li><li> Termómetro </li><li> Temporizador </li><li> Cámara de video </li><li> Generador de ruido blanco (opcional) </li><li> Secado de papel </li></ul>

the mouse forced swim test

La prueba de natación forzada es una prueba de roedores utilizados para la evaluación del comportamiento de los fármacos antidepresivos, la eficacia antidepresiva de nuevos compuestos, y las manipulaciones experimentales que tienen por objeto la prestación o la prevención de estados depresivos-como. Los ratones se colocan en un tanque transparente ineludible que se llena de agua y su comportamiento de huida relacionadas con la movilidad se mide. La prueba de natación forzada es sencillo de llevar a cabo de forma fiable y requiere un mínimo de equipo especializado. La implementación exitosa de la prueba de natación forzada requiere la adhesión a ciertos detalles de procedimiento y la minimización del estrés injustificado a los ratones. En la descripción del protocolo y el vídeo que acompaña, se explica cómo llevar a cabo la versión de ratón de esta prueba, con énfasis en peligros potenciales que pueden ser perjudiciales para la interpretación de los resultados y cómo evitarlos. Además, se explica cómo el comportamiento se manifiesta en la prueba se evalúan.

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

La prueba de natación forzada es validado como un método experimental para evaluar la eficacia de los antidepresivos potencial en los roedores. Animales de experimentación se colocan en un tanque de agua y escapar de comportamiento relacionado con la movilidad se cuantifica. Los procedimientos comunes para la versión de ratón de esta prueba se describen.

El ratón de prueba de natación forzada

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

Research

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Infusión estereotáxica de Oligomeric beta-amiloide en el hipocampo del ratón

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