Name: the mouse forced swim test
Uploaded: 2012-01-29T14:00:00
Description: Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

Denna studie har fått stöd av bidraget NIHM R01 MH091816 och R21 MH084043 till TDG.

1. Material och Metod 1,1. Vattnet tankar Den cylindriska tankar (30 cm höjd x 20 cm diameter) som krävs för den påtvingade mus simma test (FST) i vårt laboratorium är konstruerade av transparent plexiglas, eftersom detta material kan motstå frekventa förflyttningar av stridsvagnar och olyckor bättre än glas. Vattennivån är 15 cm från botten och skall märkas på tanken att se till att den volym vatten är enhetlig i möss. Antalet tankar bör helst vara ...

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FST (som ibland kallas Porsolt simma test) utvecklades först för råttor och sedan modifierats för möss med Porsolt och kollegor 12,13. Förutom de ovan beskrivna protokoll framgångsrika i vårt laboratorium, har ett antal till stor del subtila testa ändringar har publicerats (se Hascoët och Bourin för en komplett recension 1). Det är ett vanligt test som används för utvärdering av effekten av antidepressiva läkemedel och effekterna av olika beteenden och neurobiologiska manipulationer...

<ul><li> Vattentankar </li><li> Termometer </li><li> Timer </li><li> Videokamera </li><li> White Noise Generator (tillval) </li><li> Torkning Paper </li></ul>

the mouse forced swim test

De tvingas simma test är en gnagare beteende test som används för utvärdering av antidepressiva läkemedel, antidepressiva effekten av nya föreningar och experimentella manipulationer som syftar till att göra eller förhindra depressiva-liknande stater. Möss placeras i ett ofrånkomligt genomskinlig tank som är fylld med vatten och deras flykt relaterade rörlighet beteenden mäts. De tvingas simma testet är enkelt att göra tillförlitligt sätt och det kräver minimalt specialutrustning. Ett framgångsrikt genomförande av den påtvingade simma test kräver anslutning till vissa processuella detaljer och minimering av obefogade stress till möss. I protokollet beskrivning och tillhörande video, förklarar vi hur man genomför musen versionen av detta test med betoning på potentiella fallgropar som kan vara skadliga för tolkning av resultat och hur man undviker dem. Dessutom förklarar vi hur de beteenden visar sig i testet bedöms.

Watch this Scientific Journal Video about The Mouse Forced Swim Test at JoVE.com

The Mouse Forced Swim Test

De tvingas simma test valideras som en experimentell metod för att utvärdera potentiella antidepressiva effekten hos gnagare. Försöksdjur placeras i en tank med vatten och fly-relaterade rörlighet beteende är kvantifieras. Den gemensamma förfaranden för musen versionen av detta test beskrivs.

Musen Tvingad Simma Test

The forced swim test is a rodent behavioral test used for evaluation of antidepressant drugs, antidepressant efficacy of new compounds, and experimental ...

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